Alignment Stats
| Sequence pairs analysed in total |
252481025 |
| Paired-end alignments with a unique best hit |
226022229 |
| Pairs without alignments under any condition |
12971139 |
| Pairs that did not map uniquely |
13487657 |
| Genomic sequence context not extractable (edges of chromosomes) |
1622 |
|
|
Cytosine Methylation
| Total C's analysed |
11680016164 |
| Methylated C's in CpG context |
410593421 |
| Methylated C's in CHG context |
15122596 |
| Methylated C's in CHH context |
54468927 |
| Methylated C's in Unknown context |
1608815 |
| Unmethylated C's in CpG context |
122824716 |
| Unmethylated C's in CHG context |
2464635871 |
| Unmethylated C's in CHH context |
8612370633 |
| Unmethylated C's in Unknown context |
7005080 |
| Percentage methylation (CpG context) |
77.0% |
| Percentage methylation (CHG context) |
0.6% |
| Percentage methylation (CHH context) |
0.6% |
| Methylated C's in Unknown context |
N/A% |
|
|
Alignment to Individual Bisulfite Strands
| OT |
113405821 |
original top strand |
| CTOT |
0 |
complementary to original top strand |
| CTOB |
0 |
complementary to original bottom strand |
| OB |
112614786 |
original bottom strand |
|
|
Deduplication
| Alignments analysed |
226020607 |
| Unique alignments |
186010902 |
| Duplicates removed |
40009705 |
| Duplicated alignments were found at 33518362 different positions |
|
|
Cytosine Methylation after Extraction
| Total C's analysed |
7065093145 |
| Methylated C's in CpG context |
247602488 |
| Methylated C's in CHG context |
11263491 |
| Methylated C's in CHH context |
41049541 |
| Unmethylated C's in CpG context |
73932330 |
| Unmethylated C's in CHG context |
1485975424 |
| Unmethylated C's in CHH context |
5205269871 |
| Percentage methylation (CpG context) |
77.0% |
| Percentage methylation (CHG context) |
N/A% |
| Percentage methylation (CHH context) |
N/A% |
|
|
Nucleotide Coverage
| Nucleotide Class | Counts Sample | Counts Genome | % in Sample | % in Genome | fold coverage |
| A | 16455460938 | 866420001 | 29.70 | 29.52 | 18.992 |
| T | 16883067832 | 868918077 | 30.48 | 29.61 | 19.430 |
| C | 10766346887 | 598683433 | 19.44 | 20.40 | 17.983 |
| G | 11291473583 | 600854940 | 20.38 | 20.47 | 18.792 |
| AC | 2694777899 | 147939993 | 4.90 | 5.04 | 18.215 |
| CA | 3870821902 | 212726856 | 7.04 | 7.25 | 18.196 |
| TC | 3189265394 | 174696228 | 5.80 | 5.95 | 18.256 |
| CT | 3732013227 | 205281198 | 6.79 | 6.99 | 18.180 |
| CC | 2575457946 | 151371046 | 4.68 | 5.16 | 17.014 |
| CG | 490544050 | 29303965 | 0.89 | 1.00 | 16.740 |
| GC | 2241499725 | 124675968 | 4.08 | 4.25 | 17.979 |
| GG | 2760870858 | 152424560 | 5.02 | 5.19 | 18.113 |
| AG | 3806708363 | 205417428 | 6.92 | 7.00 | 18.532 |
| GA | 3293525993 | 175501875 | 5.99 | 5.98 | 18.766 |
| TG | 4136265834 | 213708670 | 7.52 | 7.28 | 19.355 |
| GT | 2918039517 | 148252316 | 5.31 | 5.05 | 19.683 |
| TT | 5648378858 | 289149328 | 10.27 | 9.85 | 19.534 |
| TA | 3762568961 | 191363599 | 6.84 | 6.52 | 19.662 |
| AT | 4486684004 | 226234956 | 8.16 | 7.71 | 19.832 |
| AA | 5375592699 | 286827281 | 9.78 | 9.77 | 18.742 |
|
|
M-Bias Plot