Bismark

Bismark Processing Report

1_trim/HWVNFDSXY_TTCCATTGAT-ACTATGCCAG_L004_R1.fq.gz and 1_trim/HWVNFDSXY_TTCCATTGAT-ACTATGCCAG_L004_R2.fq.gz

Data processed at 08:46:30 on 2021-08-03


Alignment Stats

Sequence pairs analysed in total 252481025
Paired-end alignments with a unique best hit 226022229
Pairs without alignments under any condition 12971139
Pairs that did not map uniquely 13487657
Genomic sequence context not extractable (edges of chromosomes) 1622

Cytosine Methylation

Total C's analysed 11680016164
Methylated C's in CpG context 410593421
Methylated C's in CHG context 15122596
Methylated C's in CHH context 54468927
Methylated C's in Unknown context 1608815
Unmethylated C's in CpG context 122824716
Unmethylated C's in CHG context 2464635871
Unmethylated C's in CHH context 8612370633
Unmethylated C's in Unknown context 7005080
Percentage methylation (CpG context) 77.0%
Percentage methylation (CHG context) 0.6%
Percentage methylation (CHH context) 0.6%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 113405821 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 112614786 original bottom strand

Deduplication

Alignments analysed 226020607
Unique alignments 186010902
Duplicates removed 40009705
Duplicated alignments were found at 33518362 different positions

Cytosine Methylation after Extraction

Total C's analysed 7065093145
Methylated C's in CpG context 247602488
Methylated C's in CHG context 11263491
Methylated C's in CHH context 41049541
Unmethylated C's in CpG context 73932330
Unmethylated C's in CHG context 1485975424
Unmethylated C's in CHH context 5205269871
Percentage methylation (CpG context) 77.0%
Percentage methylation (CHG context) N/A%
Percentage methylation (CHH context) N/A%

Nucleotide Coverage

Nucleotide Class Counts Sample Counts Genome% in Sample % in Genome fold coverage
A 16455460938 866420001 29.70 29.52 18.992
T 16883067832 868918077 30.48 29.61 19.430
C 10766346887 598683433 19.44 20.40 17.983
G 11291473583 600854940 20.38 20.47 18.792
AC 2694777899 147939993 4.90 5.04 18.215
CA 3870821902 212726856 7.04 7.25 18.196
TC 3189265394 174696228 5.80 5.95 18.256
CT 3732013227 205281198 6.79 6.99 18.180
CC 2575457946 151371046 4.68 5.16 17.014
CG 490544050 29303965 0.89 1.00 16.740
GC 2241499725 124675968 4.08 4.25 17.979
GG 2760870858 152424560 5.02 5.19 18.113
AG 3806708363 205417428 6.92 7.00 18.532
GA 3293525993 175501875 5.99 5.98 18.766
TG 4136265834 213708670 7.52 7.28 19.355
GT 2918039517 148252316 5.31 5.05 19.683
TT 5648378858 289149328 10.27 9.85 19.534
TA 3762568961 191363599 6.84 6.52 19.662
AT 4486684004 226234956 8.16 7.71 19.832
AA 5375592699 286827281 9.78 9.77 18.742

M-Bias Plot