Bismark

Bismark Processing Report

1_trim/HWVNFDSXY_TGTGGTTGAT-ACTCAGATTC_L003_R1.fq.gz and 1_trim/HWVNFDSXY_TGTGGTTGAT-ACTCAGATTC_L003_R2.fq.gz

Data processed at 01:14:46 on 2021-07-20


Alignment Stats

Sequence pairs analysed in total 273122788
Paired-end alignments with a unique best hit 243941449
Pairs without alignments under any condition 15427060
Pairs that did not map uniquely 13754279
Genomic sequence context not extractable (edges of chromosomes) 2133

Cytosine Methylation

Total C's analysed 12548936971
Methylated C's in CpG context 455050395
Methylated C's in CHG context 14628409
Methylated C's in CHH context 52711075
Methylated C's in Unknown context 1475858
Unmethylated C's in CpG context 107793070
Unmethylated C's in CHG context 2633691627
Unmethylated C's in CHH context 9285062395
Unmethylated C's in Unknown context 7023289
Percentage methylation (CpG context) 80.8%
Percentage methylation (CHG context) 0.6%
Percentage methylation (CHH context) 0.6%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 122416083 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 121523233 original bottom strand

Deduplication

Alignments analysed 243939316
Unique alignments 196025679
Duplicates removed 47913637
Duplicated alignments were found at 39136638 different positions

Cytosine Methylation after Extraction

Total C's analysed 7823891984
Methylated C's in CpG context 282959569
Methylated C's in CHG context 10685387
Methylated C's in CHH context 38906813
Unmethylated C's in CpG context 66693667
Unmethylated C's in CHG context 1637430291
Unmethylated C's in CHH context 5787216257
Percentage methylation (CpG context) 80.9%
Percentage methylation (CHG context) N/A%
Percentage methylation (CHH context) N/A%

Nucleotide Coverage

Nucleotide Class Counts Sample Counts Genome% in Sample % in Genome fold coverage
A 18120617248 866420001 30.01 29.52 20.914
T 18408743371 868918077 30.49 29.61 21.186
C 11639679572 598683433 19.28 20.40 19.442
G 12212187420 600854940 20.23 20.47 20.325
AC 2946379417 147939993 4.92 5.04 19.916
CA 4216316340 212726856 7.04 7.25 19.820
TC 3454752378 174696228 5.76 5.95 19.776
CT 4035308861 205281198 6.73 6.99 19.657
CC 2761607119 151371046 4.61 5.16 18.244
CG 521109788 29303965 0.87 1.00 17.783
GC 2406788512 124675968 4.02 4.25 19.304
GG 2964699047 152424560 4.95 5.19 19.450
AG 4148705493 205417428 6.92 7.00 20.196
GA 3598606705 175501875 6.00 5.98 20.505
TG 4472242861 213708670 7.46 7.28 20.927
GT 3158347356 148252316 5.27 5.05 21.304
TT 6163921844 289149328 10.28 9.85 21.317
TA 4158168381 191363599 6.94 6.52 21.729
AT 4945095019 226234956 8.25 7.71 21.858
AA 5980199962 286827281 9.98 9.77 20.849

M-Bias Plot