Alignment Stats
| Sequence pairs analysed in total |
273122788 |
| Paired-end alignments with a unique best hit |
243941449 |
| Pairs without alignments under any condition |
15427060 |
| Pairs that did not map uniquely |
13754279 |
| Genomic sequence context not extractable (edges of chromosomes) |
2133 |
|
|
Cytosine Methylation
| Total C's analysed |
12548936971 |
| Methylated C's in CpG context |
455050395 |
| Methylated C's in CHG context |
14628409 |
| Methylated C's in CHH context |
52711075 |
| Methylated C's in Unknown context |
1475858 |
| Unmethylated C's in CpG context |
107793070 |
| Unmethylated C's in CHG context |
2633691627 |
| Unmethylated C's in CHH context |
9285062395 |
| Unmethylated C's in Unknown context |
7023289 |
| Percentage methylation (CpG context) |
80.8% |
| Percentage methylation (CHG context) |
0.6% |
| Percentage methylation (CHH context) |
0.6% |
| Methylated C's in Unknown context |
N/A% |
|
|
Alignment to Individual Bisulfite Strands
| OT |
122416083 |
original top strand |
| CTOT |
0 |
complementary to original top strand |
| CTOB |
0 |
complementary to original bottom strand |
| OB |
121523233 |
original bottom strand |
|
|
Deduplication
| Alignments analysed |
243939316 |
| Unique alignments |
196025679 |
| Duplicates removed |
47913637 |
| Duplicated alignments were found at 39136638 different positions |
|
|
Cytosine Methylation after Extraction
| Total C's analysed |
7823891984 |
| Methylated C's in CpG context |
282959569 |
| Methylated C's in CHG context |
10685387 |
| Methylated C's in CHH context |
38906813 |
| Unmethylated C's in CpG context |
66693667 |
| Unmethylated C's in CHG context |
1637430291 |
| Unmethylated C's in CHH context |
5787216257 |
| Percentage methylation (CpG context) |
80.9% |
| Percentage methylation (CHG context) |
N/A% |
| Percentage methylation (CHH context) |
N/A% |
|
|
Nucleotide Coverage
| Nucleotide Class | Counts Sample | Counts Genome | % in Sample | % in Genome | fold coverage |
| A | 18120617248 | 866420001 | 30.01 | 29.52 | 20.914 |
| T | 18408743371 | 868918077 | 30.49 | 29.61 | 21.186 |
| C | 11639679572 | 598683433 | 19.28 | 20.40 | 19.442 |
| G | 12212187420 | 600854940 | 20.23 | 20.47 | 20.325 |
| AC | 2946379417 | 147939993 | 4.92 | 5.04 | 19.916 |
| CA | 4216316340 | 212726856 | 7.04 | 7.25 | 19.820 |
| TC | 3454752378 | 174696228 | 5.76 | 5.95 | 19.776 |
| CT | 4035308861 | 205281198 | 6.73 | 6.99 | 19.657 |
| CC | 2761607119 | 151371046 | 4.61 | 5.16 | 18.244 |
| CG | 521109788 | 29303965 | 0.87 | 1.00 | 17.783 |
| GC | 2406788512 | 124675968 | 4.02 | 4.25 | 19.304 |
| GG | 2964699047 | 152424560 | 4.95 | 5.19 | 19.450 |
| AG | 4148705493 | 205417428 | 6.92 | 7.00 | 20.196 |
| GA | 3598606705 | 175501875 | 6.00 | 5.98 | 20.505 |
| TG | 4472242861 | 213708670 | 7.46 | 7.28 | 20.927 |
| GT | 3158347356 | 148252316 | 5.27 | 5.05 | 21.304 |
| TT | 6163921844 | 289149328 | 10.28 | 9.85 | 21.317 |
| TA | 4158168381 | 191363599 | 6.94 | 6.52 | 21.729 |
| AT | 4945095019 | 226234956 | 8.25 | 7.71 | 21.858 |
| AA | 5980199962 | 286827281 | 9.98 | 9.77 | 20.849 |
|
|
M-Bias Plot