Alignment Stats
| Sequence pairs analysed in total |
343087716 |
| Paired-end alignments with a unique best hit |
308261504 |
| Pairs without alignments under any condition |
17322916 |
| Pairs that did not map uniquely |
17503296 |
| Genomic sequence context not extractable (edges of chromosomes) |
1895 |
|
|
Cytosine Methylation
| Total C's analysed |
15481791808 |
| Methylated C's in CpG context |
542717050 |
| Methylated C's in CHG context |
22932729 |
| Methylated C's in CHH context |
84871483 |
| Methylated C's in Unknown context |
2629668 |
| Unmethylated C's in CpG context |
137696054 |
| Unmethylated C's in CHG context |
3228078008 |
| Unmethylated C's in CHH context |
11465496484 |
| Unmethylated C's in Unknown context |
10390062 |
| Percentage methylation (CpG context) |
79.8% |
| Percentage methylation (CHG context) |
0.7% |
| Percentage methylation (CHH context) |
0.7% |
| Methylated C's in Unknown context |
N/A% |
|
|
Alignment to Individual Bisulfite Strands
| OT |
154737813 |
original top strand |
| CTOT |
0 |
complementary to original top strand |
| CTOB |
0 |
complementary to original bottom strand |
| OB |
153521796 |
original bottom strand |
|
|
Deduplication
| Alignments analysed |
308259609 |
| Unique alignments |
223974455 |
| Duplicates removed |
84285154 |
| Duplicated alignments were found at 62515444 different positions |
|
|
Cytosine Methylation after Extraction
| Total C's analysed |
8356232124 |
| Methylated C's in CpG context |
292507319 |
| Methylated C's in CHG context |
15139389 |
| Methylated C's in CHH context |
56797129 |
| Unmethylated C's in CpG context |
73857330 |
| Unmethylated C's in CHG context |
1735826387 |
| Unmethylated C's in CHH context |
6182104570 |
| Percentage methylation (CpG context) |
79.8% |
| Percentage methylation (CHG context) |
N/A% |
| Percentage methylation (CHH context) |
N/A% |
|
|
Nucleotide Coverage
| Nucleotide Class | Counts Sample | Counts Genome | % in Sample | % in Genome | fold coverage |
|---|
| A | 23508345038 | 866420001 | 30.98 | 29.52 | 27.133 |
|---|
| T | 23022507566 | 868918077 | 30.34 | 29.61 | 26.496 |
|---|
| C | 14163211054 | 598683433 | 18.66 | 20.40 | 23.657 |
|---|
| G | 15194360786 | 600854940 | 20.02 | 20.47 | 25.288 |
|---|
| AC | 3724488220 | 147939993 | 4.94 | 5.04 | 25.176 |
|---|
| CA | 5266345327 | 212726856 | 6.99 | 7.25 | 24.756 |
|---|
| TC | 4192615848 | 174696228 | 5.57 | 5.95 | 23.999 |
|---|
| CT | 4891141554 | 205281198 | 6.49 | 6.99 | 23.827 |
|---|
| CC | 3253281647 | 151371046 | 4.32 | 5.16 | 21.492 |
|---|
| CG | 619317731 | 29303965 | 0.82 | 1.00 | 21.134 |
|---|
| GC | 2908184954 | 124675968 | 3.86 | 4.25 | 23.326 |
|---|
| GG | 3639706712 | 152424560 | 4.83 | 5.19 | 23.879 |
|---|
| AG | 5264145738 | 205417428 | 6.99 | 7.00 | 25.627 |
|---|
| GA | 4615193625 | 175501875 | 6.13 | 5.98 | 26.297 |
|---|
| TG | 5542677869 | 213708670 | 7.36 | 7.28 | 25.936 |
|---|
| GT | 3929558810 | 148252316 | 5.22 | 5.05 | 26.506 |
|---|
| TT | 7683808660 | 289149328 | 10.20 | 9.85 | 26.574 |
|---|
| TA | 5407160160 | 191363599 | 7.18 | 6.52 | 28.256 |
|---|
| AT | 6387680285 | 226234956 | 8.48 | 7.71 | 28.235 |
|---|
| AA | 8004775154 | 286827281 | 10.63 | 9.77 | 27.908 |
|---|
|
|
M-Bias Plot
