Alignment Stats
| Sequence pairs analysed in total |
281437455 |
| Paired-end alignments with a unique best hit |
253814591 |
| Pairs without alignments under any condition |
15300489 |
| Pairs that did not map uniquely |
12322375 |
| Genomic sequence context not extractable (edges of chromosomes) |
2002 |
|
|
Cytosine Methylation
| Total C's analysed |
11709539086 |
| Methylated C's in CpG context |
416792452 |
| Methylated C's in CHG context |
13866032 |
| Methylated C's in CHH context |
52447845 |
| Methylated C's in Unknown context |
1572054 |
| Unmethylated C's in CpG context |
89211688 |
| Unmethylated C's in CHG context |
2382382136 |
| Unmethylated C's in CHH context |
8754838933 |
| Unmethylated C's in Unknown context |
7253786 |
| Percentage methylation (CpG context) |
82.4% |
| Percentage methylation (CHG context) |
0.6% |
| Percentage methylation (CHH context) |
0.6% |
| Methylated C's in Unknown context |
N/A% |
|
|
Alignment to Individual Bisulfite Strands
| OT |
127372285 |
original top strand |
| CTOT |
0 |
complementary to original top strand |
| CTOB |
0 |
complementary to original bottom strand |
| OB |
126440304 |
original bottom strand |
|
|
Deduplication
| Alignments analysed |
253812589 |
| Unique alignments |
170258404 |
| Duplicates removed |
83554185 |
| Duplicated alignments were found at 57891200 different positions |
|
|
Cytosine Methylation after Extraction
| Total C's analysed |
6060962422 |
| Methylated C's in CpG context |
215315836 |
| Methylated C's in CHG context |
8641204 |
| Methylated C's in CHH context |
32980494 |
| Unmethylated C's in CpG context |
45578070 |
| Unmethylated C's in CHG context |
1231468111 |
| Unmethylated C's in CHH context |
4526978707 |
| Percentage methylation (CpG context) |
82.5% |
| Percentage methylation (CHG context) |
N/A% |
| Percentage methylation (CHH context) |
N/A% |
|
|
Nucleotide Coverage
| Nucleotide Class | Counts Sample | Counts Genome | % in Sample | % in Genome | fold coverage |
|---|
| A | 21093565620 | 866420001 | 33.71 | 29.52 | 24.346 |
|---|
| T | 18458233338 | 868918077 | 29.50 | 29.61 | 21.243 |
|---|
| C | 10834856681 | 598683433 | 17.31 | 20.40 | 18.098 |
|---|
| G | 12193554065 | 600854940 | 19.48 | 20.47 | 20.294 |
|---|
| AC | 3113477218 | 147939993 | 5.01 | 5.04 | 21.046 |
|---|
| CA | 4300717735 | 212726856 | 6.92 | 7.25 | 20.217 |
|---|
| TC | 3168814608 | 174696228 | 5.10 | 5.95 | 18.139 |
|---|
| CT | 3649291063 | 205281198 | 5.88 | 6.99 | 17.777 |
|---|
| CC | 2319453533 | 151371046 | 3.73 | 5.16 | 15.323 |
|---|
| CG | 465171991 | 29303965 | 0.75 | 1.00 | 15.874 |
|---|
| GC | 2168806384 | 124675968 | 3.49 | 4.25 | 17.396 |
|---|
| GG | 2857194973 | 152424560 | 4.60 | 5.19 | 18.745 |
|---|
| AG | 4437829295 | 205417428 | 7.14 | 7.00 | 21.604 |
|---|
| GA | 4029361285 | 175501875 | 6.49 | 5.98 | 22.959 |
|---|
| TG | 4329186388 | 213708670 | 6.97 | 7.28 | 20.257 |
|---|
| GT | 3053855836 | 148252316 | 4.92 | 5.05 | 20.599 |
|---|
| TT | 6015691273 | 289149328 | 9.68 | 9.85 | 20.805 |
|---|
| TA | 4782510679 | 191363599 | 7.70 | 6.52 | 24.992 |
|---|
| AT | 5635705744 | 226234956 | 9.07 | 7.71 | 24.911 |
|---|
| AA | 7787593813 | 286827281 | 12.54 | 9.77 | 27.151 |
|---|
|
|
M-Bias Plot
