Alignment Stats
| Sequence pairs analysed in total |
302657045 |
| Paired-end alignments with a unique best hit |
270780400 |
| Pairs without alignments under any condition |
16770165 |
| Pairs that did not map uniquely |
15106480 |
| Genomic sequence context not extractable (edges of chromosomes) |
1562 |
|
|
Cytosine Methylation
| Total C's analysed |
14057168878 |
| Methylated C's in CpG context |
506729965 |
| Methylated C's in CHG context |
16929242 |
| Methylated C's in CHH context |
61236227 |
| Methylated C's in Unknown context |
1829572 |
| Unmethylated C's in CpG context |
126834942 |
| Unmethylated C's in CHG context |
2956082254 |
| Unmethylated C's in CHH context |
10389356248 |
| Unmethylated C's in Unknown context |
8250843 |
| Percentage methylation (CpG context) |
80.0% |
| Percentage methylation (CHG context) |
0.6% |
| Percentage methylation (CHH context) |
0.6% |
| Methylated C's in Unknown context |
N/A% |
|
|
Alignment to Individual Bisulfite Strands
| OT |
135892787 |
original top strand |
| CTOT |
0 |
complementary to original top strand |
| CTOB |
0 |
complementary to original bottom strand |
| OB |
134886051 |
original bottom strand |
|
|
Deduplication
| Alignments analysed |
270778838 |
| Unique alignments |
221282080 |
| Duplicates removed |
49496758 |
| Duplicated alignments were found at 41131030 different positions |
|
|
Cytosine Methylation after Extraction
| Total C's analysed |
8574909803 |
| Methylated C's in CpG context |
308211137 |
| Methylated C's in CHG context |
12527796 |
| Methylated C's in CHH context |
45831165 |
| Unmethylated C's in CpG context |
76814672 |
| Unmethylated C's in CHG context |
1797185595 |
| Unmethylated C's in CHH context |
6334339438 |
| Percentage methylation (CpG context) |
80.0% |
| Percentage methylation (CHG context) |
N/A% |
| Percentage methylation (CHH context) |
N/A% |
|
|
Nucleotide Coverage
| Nucleotide Class | Counts Sample | Counts Genome | % in Sample | % in Genome | fold coverage |
|---|
| A | 19745682583 | 866420001 | 29.66 | 29.52 | 22.790 |
|---|
| T | 20345842816 | 868918077 | 30.56 | 29.61 | 23.415 |
|---|
| C | 12988260976 | 598683433 | 19.51 | 20.40 | 21.695 |
|---|
| G | 13502813239 | 600854940 | 20.28 | 20.47 | 22.473 |
|---|
| AC | 3243348708 | 147939993 | 4.91 | 5.04 | 21.923 |
|---|
| CA | 4657995791 | 212726856 | 7.05 | 7.25 | 21.897 |
|---|
| TC | 3856316518 | 174696228 | 5.84 | 5.95 | 22.074 |
|---|
| CT | 4511265114 | 205281198 | 6.83 | 6.99 | 21.976 |
|---|
| CC | 3116386049 | 151371046 | 4.72 | 5.16 | 20.588 |
|---|
| CG | 584946356 | 29303965 | 0.89 | 1.00 | 19.961 |
|---|
| GC | 2693576731 | 124675968 | 4.08 | 4.25 | 21.605 |
|---|
| GG | 3286201158 | 152424560 | 4.97 | 5.19 | 21.560 |
|---|
| AG | 4554413779 | 205417428 | 6.89 | 7.00 | 22.172 |
|---|
| GA | 3929668616 | 175501875 | 5.95 | 5.98 | 22.391 |
|---|
| TG | 4960547968 | 213708670 | 7.51 | 7.28 | 23.212 |
|---|
| GT | 3501173132 | 148252316 | 5.30 | 5.05 | 23.616 |
|---|
| TT | 6821789432 | 289149328 | 10.32 | 9.85 | 23.593 |
|---|
| TA | 4530278545 | 191363599 | 6.86 | 6.52 | 23.674 |
|---|
| AT | 5393614811 | 226234956 | 8.16 | 7.71 | 23.841 |
|---|
| AA | 6444697736 | 286827281 | 9.75 | 9.77 | 22.469 |
|---|
|
|
M-Bias Plot
