Bismark

Bismark Processing Report

1_trim/HWVNFDSXY_CTCTATCGAT-ACCCACCGAT_L003_R1.fq.gz and 1_trim/HWVNFDSXY_CTCTATCGAT-ACCCACCGAT_L003_R2.fq.gz

Data processed at 03:29:04 on 2021-07-22


Alignment Stats

Sequence pairs analysed in total 240121623
Paired-end alignments with a unique best hit 215661671
Pairs without alignments under any condition 12537661
Pairs that did not map uniquely 11922291
Genomic sequence context not extractable (edges of chromosomes) 2123

Cytosine Methylation

Total C's analysed 10683890105
Methylated C's in CpG context 385387099
Methylated C's in CHG context 13357375
Methylated C's in CHH context 48905155
Methylated C's in Unknown context 1418707
Unmethylated C's in CpG context 83859687
Unmethylated C's in CHG context 2226342843
Unmethylated C's in CHH context 7926037946
Unmethylated C's in Unknown context 6473834
Percentage methylation (CpG context) 82.1%
Percentage methylation (CHG context) 0.6%
Percentage methylation (CHH context) 0.6%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 108266912 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 107392636 original bottom strand

Deduplication

Alignments analysed 215659548
Unique alignments 178828624
Duplicates removed 36830924
Duplicated alignments were found at 30907780 different positions

Cytosine Methylation after Extraction

Total C's analysed 6640076658
Methylated C's in CpG context 239082106
Methylated C's in CHG context 9973937
Methylated C's in CHH context 36864105
Unmethylated C's in CpG context 51689768
Unmethylated C's in CHG context 1380238200
Unmethylated C's in CHH context 4922228542
Percentage methylation (CpG context) 82.2%
Percentage methylation (CHG context) N/A%
Percentage methylation (CHH context) N/A%

Nucleotide Coverage

Nucleotide Class Counts Sample Counts Genome% in Sample % in Genome fold coverage
A 16426270346 866420001 30.98 29.52 18.959
T 16098330965 868918077 30.36 29.61 18.527
C 9878563185 598683433 18.63 20.40 16.500
G 10614776380 600854940 20.02 20.47 17.666
AC 2598965109 147939993 4.94 5.04 17.568
CA 3678880237 212726856 6.99 7.25 17.294
TC 2928537232 174696228 5.57 5.95 16.764
CT 3411382923 205281198 6.48 6.99 16.618
CC 2265313079 151371046 4.30 5.16 14.965
CG 432819892 29303965 0.82 1.00 14.770
GC 2026142635 124675968 3.85 4.25 16.251
GG 2548187076 152424560 4.84 5.19 16.718
AG 3673899732 205417428 6.98 7.00 17.885
GA 3225300190 175501875 6.13 5.98 18.378
TG 3868622749 213708670 7.35 7.28 18.102
GT 2742476153 148252316 5.21 5.05 18.499
TT 5374832976 289149328 10.21 9.85 18.588
TA 3785686682 191363599 7.19 6.52 19.783
AT 4477120020 226234956 8.51 7.71 19.790
AA 5584454943 286827281 10.61 9.77 19.470

M-Bias Plot