Alignment Stats
| Sequence pairs analysed in total |
240121623 |
| Paired-end alignments with a unique best hit |
215661671 |
| Pairs without alignments under any condition |
12537661 |
| Pairs that did not map uniquely |
11922291 |
| Genomic sequence context not extractable (edges of chromosomes) |
2123 |
|
|
Cytosine Methylation
| Total C's analysed |
10683890105 |
| Methylated C's in CpG context |
385387099 |
| Methylated C's in CHG context |
13357375 |
| Methylated C's in CHH context |
48905155 |
| Methylated C's in Unknown context |
1418707 |
| Unmethylated C's in CpG context |
83859687 |
| Unmethylated C's in CHG context |
2226342843 |
| Unmethylated C's in CHH context |
7926037946 |
| Unmethylated C's in Unknown context |
6473834 |
| Percentage methylation (CpG context) |
82.1% |
| Percentage methylation (CHG context) |
0.6% |
| Percentage methylation (CHH context) |
0.6% |
| Methylated C's in Unknown context |
N/A% |
|
|
Alignment to Individual Bisulfite Strands
| OT |
108266912 |
original top strand |
| CTOT |
0 |
complementary to original top strand |
| CTOB |
0 |
complementary to original bottom strand |
| OB |
107392636 |
original bottom strand |
|
|
Deduplication
| Alignments analysed |
215659548 |
| Unique alignments |
178828624 |
| Duplicates removed |
36830924 |
| Duplicated alignments were found at 30907780 different positions |
|
|
Cytosine Methylation after Extraction
| Total C's analysed |
6640076658 |
| Methylated C's in CpG context |
239082106 |
| Methylated C's in CHG context |
9973937 |
| Methylated C's in CHH context |
36864105 |
| Unmethylated C's in CpG context |
51689768 |
| Unmethylated C's in CHG context |
1380238200 |
| Unmethylated C's in CHH context |
4922228542 |
| Percentage methylation (CpG context) |
82.2% |
| Percentage methylation (CHG context) |
N/A% |
| Percentage methylation (CHH context) |
N/A% |
|
|
Nucleotide Coverage
| Nucleotide Class | Counts Sample | Counts Genome | % in Sample | % in Genome | fold coverage |
| A | 16426270346 | 866420001 | 30.98 | 29.52 | 18.959 |
| T | 16098330965 | 868918077 | 30.36 | 29.61 | 18.527 |
| C | 9878563185 | 598683433 | 18.63 | 20.40 | 16.500 |
| G | 10614776380 | 600854940 | 20.02 | 20.47 | 17.666 |
| AC | 2598965109 | 147939993 | 4.94 | 5.04 | 17.568 |
| CA | 3678880237 | 212726856 | 6.99 | 7.25 | 17.294 |
| TC | 2928537232 | 174696228 | 5.57 | 5.95 | 16.764 |
| CT | 3411382923 | 205281198 | 6.48 | 6.99 | 16.618 |
| CC | 2265313079 | 151371046 | 4.30 | 5.16 | 14.965 |
| CG | 432819892 | 29303965 | 0.82 | 1.00 | 14.770 |
| GC | 2026142635 | 124675968 | 3.85 | 4.25 | 16.251 |
| GG | 2548187076 | 152424560 | 4.84 | 5.19 | 16.718 |
| AG | 3673899732 | 205417428 | 6.98 | 7.00 | 17.885 |
| GA | 3225300190 | 175501875 | 6.13 | 5.98 | 18.378 |
| TG | 3868622749 | 213708670 | 7.35 | 7.28 | 18.102 |
| GT | 2742476153 | 148252316 | 5.21 | 5.05 | 18.499 |
| TT | 5374832976 | 289149328 | 10.21 | 9.85 | 18.588 |
| TA | 3785686682 | 191363599 | 7.19 | 6.52 | 19.783 |
| AT | 4477120020 | 226234956 | 8.51 | 7.71 | 19.790 |
| AA | 5584454943 | 286827281 | 10.61 | 9.77 | 19.470 |
|
|
M-Bias Plot