Bismark

Bismark Processing Report

1_trim/HWVNFDSXY_CTATCATGAT-ACCGAAGTCA_L004_R1.fq.gz and 1_trim/HWVNFDSXY_CTATCATGAT-ACCGAAGTCA_L004_R2.fq.gz

Data processed at 23:01:12 on 2021-07-24


Alignment Stats

Sequence pairs analysed in total 273899149
Paired-end alignments with a unique best hit 246594754
Pairs without alignments under any condition 13522777
Pairs that did not map uniquely 13781618
Genomic sequence context not extractable (edges of chromosomes) 1747

Cytosine Methylation

Total C's analysed 12585064357
Methylated C's in CpG context 461564975
Methylated C's in CHG context 14991646
Methylated C's in CHH context 54101515
Methylated C's in Unknown context 1526179
Unmethylated C's in CpG context 102749352
Unmethylated C's in CHG context 2640727970
Unmethylated C's in CHH context 9310928899
Unmethylated C's in Unknown context 7350659
Percentage methylation (CpG context) 81.8%
Percentage methylation (CHG context) 0.6%
Percentage methylation (CHH context) 0.6%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 123761310 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 122831697 original bottom strand

Deduplication

Alignments analysed 246593007
Unique alignments 202049948
Duplicates removed 44543059
Duplicated alignments were found at 36947743 different positions

Cytosine Methylation after Extraction

Total C's analysed 7796338428
Methylated C's in CpG context 285259653
Methylated C's in CHG context 11140016
Methylated C's in CHH context 40614960
Unmethylated C's in CpG context 63269241
Unmethylated C's in CHG context 1631696076
Unmethylated C's in CHH context 5764358482
Percentage methylation (CpG context) 81.8%
Percentage methylation (CHG context) N/A%
Percentage methylation (CHH context) N/A%

Nucleotide Coverage

Nucleotide Class Counts Sample Counts Genome% in Sample % in Genome fold coverage
A 18297274627 866420001 30.09 29.52 21.118
T 18544334503 868918077 30.50 29.61 21.342
C 11658594200 598683433 19.17 20.40 19.474
G 12306384614 600854940 20.24 20.47 20.481
AC 2962442237 147939993 4.91 5.04 20.025
CA 4238206926 212726856 7.02 7.25 19.923
TC 3463302127 174696228 5.74 5.95 19.825
CT 4041043030 205281198 6.70 6.99 19.685
CC 2751766132 151371046 4.56 5.16 18.179
CG 521933507 29303965 0.86 1.00 17.811
GC 2410575652 124675968 3.99 4.25 19.335
GG 2988620819 152424560 4.95 5.19 19.607
AG 4182620244 205417428 6.93 7.00 20.362
GA 3637986939 175501875 6.03 5.98 20.729
TG 4507433483 213708670 7.47 7.28 21.091
GT 3184569790 148252316 5.28 5.05 21.481
TT 6210311894 289149328 10.29 9.85 21.478
TA 4202178364 191363599 6.96 6.52 21.959
AT 5001296040 226234956 8.29 7.71 22.107
AA 6049243731 286827281 10.02 9.77 21.090

M-Bias Plot