Bismark

Bismark Processing Report

1_trim/HWVNFDSXY_CGAAGTCAAT-ACCTATCATG_L004_R1.fq.gz and 1_trim/HWVNFDSXY_CGAAGTCAAT-ACCTATCATG_L004_R2.fq.gz

Data processed at 06:21:05 on 2021-08-01


Alignment Stats

Sequence pairs analysed in total 260992707
Paired-end alignments with a unique best hit 234536038
Pairs without alignments under any condition 13252001
Pairs that did not map uniquely 13204668
Genomic sequence context not extractable (edges of chromosomes) 1672

Cytosine Methylation

Total C's analysed 11744819477
Methylated C's in CpG context 422816222
Methylated C's in CHG context 14093602
Methylated C's in CHH context 51105862
Methylated C's in Unknown context 1482370
Unmethylated C's in CpG context 97598348
Unmethylated C's in CHG context 2460046771
Unmethylated C's in CHH context 8699158672
Unmethylated C's in Unknown context 6899435
Percentage methylation (CpG context) 81.2%
Percentage methylation (CHG context) 0.6%
Percentage methylation (CHH context) 0.6%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 117733568 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 116800798 original bottom strand

Deduplication

Alignments analysed 234534366
Unique alignments 187277032
Duplicates removed 47257334
Duplicated alignments were found at 38201417 different positions

Cytosine Methylation after Extraction

Total C's analysed 7102248262
Methylated C's in CpG context 255333919
Methylated C's in CHG context 10251398
Methylated C's in CHH context 37581023
Unmethylated C's in CpG context 58645385
Unmethylated C's in CHG context 1484170058
Unmethylated C's in CHH context 5256266479
Percentage methylation (CpG context) 81.3%
Percentage methylation (CHG context) N/A%
Percentage methylation (CHH context) N/A%

Nucleotide Coverage

Nucleotide Class Counts Sample Counts Genome% in Sample % in Genome fold coverage
A 17783466483 866420001 30.75 29.52 20.525
T 17528096437 868918077 30.30 29.61 20.172
C 10876568999 598683433 18.80 20.40 18.167
G 11653167412 600854940 20.15 20.47 19.394
AC 2832350089 147939993 4.93 5.04 19.145
CA 4020898149 212726856 7.00 7.25 18.902
TC 3216947997 174696228 5.60 5.95 18.415
CT 3756917957 205281198 6.54 6.99 18.301
CC 2519179360 151371046 4.39 5.16 16.642
CG 480643339 29303965 0.84 1.00 16.402
GC 2242698515 124675968 3.91 4.25 17.988
GG 2816797391 152424560 4.91 5.19 18.480
AG 4018432270 205417428 7.00 7.00 19.562
GA 3515949850 175501875 6.12 5.98 20.034
TG 4237126669 213708670 7.38 7.28 19.827
GT 2997253743 148252316 5.22 5.05 20.217
TT 5846445704 289149328 10.18 9.85 20.219
TA 4075592634 191363599 7.10 6.52 21.298
AT 4826509503 226234956 8.41 7.71 21.334
AA 6006976203 286827281 10.46 9.77 20.943

M-Bias Plot