Bismark

Bismark Processing Report

1_trim/HWVNFDSXY_CCACCGATAT-ACCTCTATCG_L003_R1.fq.gz and 1_trim/HWVNFDSXY_CCACCGATAT-ACCTCTATCG_L003_R2.fq.gz

Data processed at 21:13:58 on 2021-07-17


Alignment Stats

Sequence pairs analysed in total 282625939
Paired-end alignments with a unique best hit 250529700
Pairs without alignments under any condition 20993009
Pairs that did not map uniquely 11103230
Genomic sequence context not extractable (edges of chromosomes) 1905

Cytosine Methylation

Total C's analysed 11313240380
Methylated C's in CpG context 396749874
Methylated C's in CHG context 14315564
Methylated C's in CHH context 54205522
Methylated C's in Unknown context 1504769
Unmethylated C's in CpG context 90300056
Unmethylated C's in CHG context 2277716643
Unmethylated C's in CHH context 8479952721
Unmethylated C's in Unknown context 7159494
Percentage methylation (CpG context) 81.5%
Percentage methylation (CHG context) 0.6%
Percentage methylation (CHH context) 0.6%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 125408652 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 125119143 original bottom strand

Deduplication

Alignments analysed 250527795
Unique alignments 168586121
Duplicates removed 81941674
Duplicated alignments were found at 56968499 different positions

Cytosine Methylation after Extraction

Total C's analysed 6084286670
Methylated C's in CpG context 213435311
Methylated C's in CHG context 8799456
Methylated C's in CHH context 33607645
Unmethylated C's in CpG context 47701462
Unmethylated C's in CHG context 1222788445
Unmethylated C's in CHH context 4557954351
Percentage methylation (CpG context) 81.7%
Percentage methylation (CHG context) N/A%
Percentage methylation (CHH context) N/A%

Nucleotide Coverage

Nucleotide Class Counts Sample Counts Genome% in Sample % in Genome fold coverage
A 21450540187 866420001 34.78 29.52 24.758
T 17897173894 868918077 29.02 29.61 20.597
C 10465584085 598683433 16.97 20.40 17.481
G 11868300448 600854940 19.24 20.47 19.752
AC 3085785784 147939993 5.04 5.04 20.858
CA 4224741746 212726856 6.90 7.25 19.860
TC 3026909834 174696228 4.94 5.95 17.327
CT 3476590148 205281198 5.68 6.99 16.936
CC 2219572806 151371046 3.63 5.16 14.663
CG 447438772 29303965 0.73 1.00 15.269
GC 2071464379 124675968 3.38 4.25 16.615
GG 2757495172 152424560 4.50 5.19 18.091
AG 4401877513 205417428 7.19 7.00 21.429
GA 4038350061 175501875 6.60 5.98 23.010
TG 4160186254 213708670 6.80 7.28 19.467
GT 2918814188 148252316 4.77 5.05 19.688
TT 5746239766 289149328 9.39 9.85 19.873
TA 4805770386 191363599 7.85 6.52 25.113
AT 5655196166 226234956 9.24 7.71 24.997
AA 8185969975 286827281 13.37 9.77 28.540

M-Bias Plot