Alignment Stats
| Sequence pairs analysed in total |
282625939 |
| Paired-end alignments with a unique best hit |
250529700 |
| Pairs without alignments under any condition |
20993009 |
| Pairs that did not map uniquely |
11103230 |
| Genomic sequence context not extractable (edges of chromosomes) |
1905 |
|
|
Cytosine Methylation
| Total C's analysed |
11313240380 |
| Methylated C's in CpG context |
396749874 |
| Methylated C's in CHG context |
14315564 |
| Methylated C's in CHH context |
54205522 |
| Methylated C's in Unknown context |
1504769 |
| Unmethylated C's in CpG context |
90300056 |
| Unmethylated C's in CHG context |
2277716643 |
| Unmethylated C's in CHH context |
8479952721 |
| Unmethylated C's in Unknown context |
7159494 |
| Percentage methylation (CpG context) |
81.5% |
| Percentage methylation (CHG context) |
0.6% |
| Percentage methylation (CHH context) |
0.6% |
| Methylated C's in Unknown context |
N/A% |
|
|
Alignment to Individual Bisulfite Strands
| OT |
125408652 |
original top strand |
| CTOT |
0 |
complementary to original top strand |
| CTOB |
0 |
complementary to original bottom strand |
| OB |
125119143 |
original bottom strand |
|
|
Deduplication
| Alignments analysed |
250527795 |
| Unique alignments |
168586121 |
| Duplicates removed |
81941674 |
| Duplicated alignments were found at 56968499 different positions |
|
|
Cytosine Methylation after Extraction
| Total C's analysed |
6084286670 |
| Methylated C's in CpG context |
213435311 |
| Methylated C's in CHG context |
8799456 |
| Methylated C's in CHH context |
33607645 |
| Unmethylated C's in CpG context |
47701462 |
| Unmethylated C's in CHG context |
1222788445 |
| Unmethylated C's in CHH context |
4557954351 |
| Percentage methylation (CpG context) |
81.7% |
| Percentage methylation (CHG context) |
N/A% |
| Percentage methylation (CHH context) |
N/A% |
|
|
Nucleotide Coverage
| Nucleotide Class | Counts Sample | Counts Genome | % in Sample | % in Genome | fold coverage |
| A | 21450540187 | 866420001 | 34.78 | 29.52 | 24.758 |
| T | 17897173894 | 868918077 | 29.02 | 29.61 | 20.597 |
| C | 10465584085 | 598683433 | 16.97 | 20.40 | 17.481 |
| G | 11868300448 | 600854940 | 19.24 | 20.47 | 19.752 |
| AC | 3085785784 | 147939993 | 5.04 | 5.04 | 20.858 |
| CA | 4224741746 | 212726856 | 6.90 | 7.25 | 19.860 |
| TC | 3026909834 | 174696228 | 4.94 | 5.95 | 17.327 |
| CT | 3476590148 | 205281198 | 5.68 | 6.99 | 16.936 |
| CC | 2219572806 | 151371046 | 3.63 | 5.16 | 14.663 |
| CG | 447438772 | 29303965 | 0.73 | 1.00 | 15.269 |
| GC | 2071464379 | 124675968 | 3.38 | 4.25 | 16.615 |
| GG | 2757495172 | 152424560 | 4.50 | 5.19 | 18.091 |
| AG | 4401877513 | 205417428 | 7.19 | 7.00 | 21.429 |
| GA | 4038350061 | 175501875 | 6.60 | 5.98 | 23.010 |
| TG | 4160186254 | 213708670 | 6.80 | 7.28 | 19.467 |
| GT | 2918814188 | 148252316 | 4.77 | 5.05 | 19.688 |
| TT | 5746239766 | 289149328 | 9.39 | 9.85 | 19.873 |
| TA | 4805770386 | 191363599 | 7.85 | 6.52 | 25.113 |
| AT | 5655196166 | 226234956 | 9.24 | 7.71 | 24.997 |
| AA | 8185969975 | 286827281 | 13.37 | 9.77 | 28.540 |
|
|
M-Bias Plot