Alignment Stats
| Sequence pairs analysed in total |
328817181 |
| Paired-end alignments with a unique best hit |
294621016 |
| Pairs without alignments under any condition |
17576023 |
| Pairs that did not map uniquely |
16620142 |
| Genomic sequence context not extractable (edges of chromosomes) |
1808 |
|
|
Cytosine Methylation
| Total C's analysed |
15657284481 |
| Methylated C's in CpG context |
580191879 |
| Methylated C's in CHG context |
20591558 |
| Methylated C's in CHH context |
73183568 |
| Methylated C's in Unknown context |
2044292 |
| Unmethylated C's in CpG context |
146933109 |
| Unmethylated C's in CHG context |
3309276001 |
| Unmethylated C's in CHH context |
11527108366 |
| Unmethylated C's in Unknown context |
9292147 |
| Percentage methylation (CpG context) |
79.8% |
| Percentage methylation (CHG context) |
0.6% |
| Percentage methylation (CHH context) |
0.6% |
| Methylated C's in Unknown context |
N/A% |
|
|
Alignment to Individual Bisulfite Strands
| OT |
147857492 |
original top strand |
| CTOT |
0 |
complementary to original top strand |
| CTOB |
0 |
complementary to original bottom strand |
| OB |
146761716 |
original bottom strand |
|
|
Deduplication
| Alignments analysed |
294619208 |
| Unique alignments |
236462637 |
| Duplicates removed |
58156571 |
| Duplicated alignments were found at 47563737 different positions |
|
|
Cytosine Methylation after Extraction
| Total C's analysed |
9425406083 |
| Methylated C's in CpG context |
347772656 |
| Methylated C's in CHG context |
14801082 |
| Methylated C's in CHH context |
53195624 |
| Unmethylated C's in CpG context |
87801197 |
| Unmethylated C's in CHG context |
1985514897 |
| Unmethylated C's in CHH context |
6936320627 |
| Percentage methylation (CpG context) |
79.8% |
| Percentage methylation (CHG context) |
N/A% |
| Percentage methylation (CHH context) |
N/A% |
|
|
Nucleotide Coverage
| Nucleotide Class | Counts Sample | Counts Genome | % in Sample | % in Genome | fold coverage |
|---|
| A | 21740077436 | 866420001 | 29.49 | 29.52 | 25.092 |
|---|
| T | 22468029420 | 868918077 | 30.48 | 29.61 | 25.857 |
|---|
| C | 14426314702 | 598683433 | 19.57 | 20.40 | 24.097 |
|---|
| G | 15078523218 | 600854940 | 20.46 | 20.47 | 25.095 |
|---|
| AC | 3574389396 | 147939993 | 4.88 | 5.04 | 24.161 |
|---|
| CA | 5155133797 | 212726856 | 7.04 | 7.25 | 24.234 |
|---|
| TC | 4277295941 | 174696228 | 5.85 | 5.95 | 24.484 |
|---|
| CT | 4994960830 | 205281198 | 6.83 | 6.99 | 24.332 |
|---|
| CC | 3476386955 | 151371046 | 4.75 | 5.16 | 22.966 |
|---|
| CG | 668218284 | 29303965 | 0.91 | 1.00 | 22.803 |
|---|
| GC | 3012359152 | 124675968 | 4.12 | 4.25 | 24.162 |
|---|
| GG | 3698931678 | 152424560 | 5.05 | 5.19 | 24.267 |
|---|
| AG | 5055994337 | 205417428 | 6.91 | 7.00 | 24.613 |
|---|
| GA | 4375626968 | 175501875 | 5.98 | 5.98 | 24.932 |
|---|
| TG | 5528235038 | 213708670 | 7.55 | 7.28 | 25.868 |
|---|
| GT | 3890650365 | 148252316 | 5.32 | 5.05 | 26.243 |
|---|
| TT | 7517757511 | 289149328 | 10.27 | 9.85 | 26.000 |
|---|
| TA | 4954804696 | 191363599 | 6.77 | 6.52 | 25.892 |
|---|
| AT | 5936807939 | 226234956 | 8.11 | 7.71 | 26.242 |
|---|
| AA | 7056860191 | 286827281 | 9.64 | 9.77 | 24.603 |
|---|
|
|
M-Bias Plot
