Alignment Stats
| Sequence pairs analysed in total |
324489233 |
| Paired-end alignments with a unique best hit |
292268828 |
| Pairs without alignments under any condition |
16184176 |
| Pairs that did not map uniquely |
16036229 |
| Genomic sequence context not extractable (edges of chromosomes) |
1872 |
|
|
Cytosine Methylation
| Total C's analysed |
15253425957 |
| Methylated C's in CpG context |
549637318 |
| Methylated C's in CHG context |
20207299 |
| Methylated C's in CHH context |
72870218 |
| Methylated C's in Unknown context |
2073639 |
| Unmethylated C's in CpG context |
131989508 |
| Unmethylated C's in CHG context |
3198014950 |
| Unmethylated C's in CHH context |
11280706664 |
| Unmethylated C's in Unknown context |
9148992 |
| Percentage methylation (CpG context) |
80.6% |
| Percentage methylation (CHG context) |
0.6% |
| Percentage methylation (CHH context) |
0.6% |
| Methylated C's in Unknown context |
N/A% |
|
|
Alignment to Individual Bisulfite Strands
| OT |
146688505 |
original top strand |
| CTOT |
0 |
complementary to original top strand |
| CTOB |
0 |
complementary to original bottom strand |
| OB |
145578451 |
original bottom strand |
|
|
Deduplication
| Alignments analysed |
292266956 |
| Unique alignments |
234947775 |
| Duplicates removed |
57319181 |
| Duplicated alignments were found at 46696566 different positions |
|
|
Cytosine Methylation after Extraction
| Total C's analysed |
9167812533 |
| Methylated C's in CpG context |
329601707 |
| Methylated C's in CHG context |
14523340 |
| Methylated C's in CHH context |
53034669 |
| Unmethylated C's in CpG context |
78906620 |
| Unmethylated C's in CHG context |
1916482139 |
| Unmethylated C's in CHH context |
6775264058 |
| Percentage methylation (CpG context) |
80.7% |
| Percentage methylation (CHG context) |
N/A% |
| Percentage methylation (CHH context) |
N/A% |
|
|
Nucleotide Coverage
| Nucleotide Class | Counts Sample | Counts Genome | % in Sample | % in Genome | fold coverage |
|---|
| A | 21917571920 | 866420001 | 30.03 | 29.52 | 25.297 |
|---|
| T | 22284573217 | 868918077 | 30.53 | 29.61 | 25.646 |
|---|
| C | 14052976877 | 598683433 | 19.25 | 20.40 | 23.473 |
|---|
| G | 14739159722 | 600854940 | 20.19 | 20.47 | 24.530 |
|---|
| AC | 3564438528 | 147939993 | 4.92 | 5.04 | 24.094 |
|---|
| CA | 5096924624 | 212726856 | 7.03 | 7.25 | 23.960 |
|---|
| TC | 4173769877 | 174696228 | 5.76 | 5.95 | 23.892 |
|---|
| CT | 4874507207 | 205281198 | 6.73 | 6.99 | 23.746 |
|---|
| CC | 3326059292 | 151371046 | 4.59 | 5.16 | 21.973 |
|---|
| CG | 626379201 | 29303965 | 0.86 | 1.00 | 21.375 |
|---|
| GC | 2905123939 | 124675968 | 4.01 | 4.25 | 23.301 |
|---|
| GG | 3568467150 | 152424560 | 4.92 | 5.19 | 23.411 |
|---|
| AG | 5011337343 | 205417428 | 6.92 | 7.00 | 24.396 |
|---|
| GA | 4344455781 | 175501875 | 6.00 | 5.98 | 24.754 |
|---|
| TG | 5408458305 | 213708670 | 7.46 | 7.28 | 25.308 |
|---|
| GT | 3822464406 | 148252316 | 5.28 | 5.05 | 25.784 |
|---|
| TT | 7473807828 | 289149328 | 10.31 | 9.85 | 25.848 |
|---|
| TA | 5039776718 | 191363599 | 6.96 | 6.52 | 26.336 |
|---|
| AT | 5987096036 | 226234956 | 8.26 | 7.71 | 26.464 |
|---|
| AA | 7237384122 | 286827281 | 9.99 | 9.77 | 25.233 |
|---|
|
|
M-Bias Plot
