Alignment Stats
| Sequence pairs analysed in total |
282024119 |
| Paired-end alignments with a unique best hit |
252073827 |
| Pairs without alignments under any condition |
15405047 |
| Pairs that did not map uniquely |
14545245 |
| Genomic sequence context not extractable (edges of chromosomes) |
1572 |
|
|
Cytosine Methylation
| Total C's analysed |
12725697391 |
| Methylated C's in CpG context |
463611169 |
| Methylated C's in CHG context |
16647730 |
| Methylated C's in CHH context |
60569613 |
| Methylated C's in Unknown context |
1823716 |
| Unmethylated C's in CpG context |
110080298 |
| Unmethylated C's in CHG context |
2667544542 |
| Unmethylated C's in CHH context |
9407244039 |
| Unmethylated C's in Unknown context |
7863118 |
| Percentage methylation (CpG context) |
80.8% |
| Percentage methylation (CHG context) |
0.6% |
| Percentage methylation (CHH context) |
0.6% |
| Methylated C's in Unknown context |
N/A% |
|
|
Alignment to Individual Bisulfite Strands
| OT |
126452355 |
original top strand |
| CTOT |
0 |
complementary to original top strand |
| CTOB |
0 |
complementary to original bottom strand |
| OB |
125619900 |
original bottom strand |
|
|
Deduplication
| Alignments analysed |
252072255 |
| Unique alignments |
204472035 |
| Duplicates removed |
47600220 |
| Duplicated alignments were found at 39315820 different positions |
|
|
Cytosine Methylation after Extraction
| Total C's analysed |
7618543612 |
| Methylated C's in CpG context |
276680859 |
| Methylated C's in CHG context |
12192896 |
| Methylated C's in CHH context |
44840921 |
| Unmethylated C's in CpG context |
65508684 |
| Unmethylated C's in CHG context |
1591862125 |
| Unmethylated C's in CHH context |
5627458127 |
| Percentage methylation (CpG context) |
80.9% |
| Percentage methylation (CHG context) |
N/A% |
| Percentage methylation (CHH context) |
N/A% |
|
|
Nucleotide Coverage
| Nucleotide Class | Counts Sample | Counts Genome | % in Sample | % in Genome | fold coverage |
| A | 18714237432 | 866420001 | 30.37 | 29.52 | 21.599 |
| T | 18711654046 | 868918077 | 30.36 | 29.61 | 21.534 |
| C | 11735206046 | 598683433 | 19.04 | 20.40 | 19.602 |
| G | 12469321022 | 600854940 | 20.23 | 20.47 | 20.753 |
| AC | 3007518058 | 147939993 | 4.92 | 5.04 | 20.329 |
| CA | 4296799226 | 212726856 | 7.02 | 7.25 | 20.199 |
| TC | 3480691268 | 174696228 | 5.69 | 5.95 | 19.924 |
| CT | 4052789096 | 205281198 | 6.63 | 6.99 | 19.743 |
| CC | 2750934168 | 151371046 | 4.50 | 5.16 | 18.173 |
| CG | 527932862 | 29303965 | 0.86 | 1.00 | 18.016 |
| GC | 2424287916 | 124675968 | 3.96 | 4.25 | 19.445 |
| GG | 3026231538 | 152424560 | 4.95 | 5.19 | 19.854 |
| AG | 4261799772 | 205417428 | 6.97 | 7.00 | 20.747 |
| GA | 3724598628 | 175501875 | 6.09 | 5.98 | 21.223 |
| TG | 4546351988 | 213708670 | 7.43 | 7.28 | 21.274 |
| GT | 3208235303 | 148252316 | 5.24 | 5.05 | 21.640 |
| TT | 6247210184 | 289149328 | 10.21 | 9.85 | 21.605 |
| TA | 4274480829 | 191363599 | 6.99 | 6.52 | 22.337 |
| AT | 5094241151 | 226234956 | 8.33 | 7.71 | 22.517 |
| AA | 6245475448 | 286827281 | 10.21 | 9.77 | 21.774 |
|
|
M-Bias Plot