Bismark

Bismark Processing Report

1_trim/HWVNFDSXY_CAGATCTGAT-ACATGTAATG_L003_R1.fq.gz and 1_trim/HWVNFDSXY_CAGATCTGAT-ACATGTAATG_L003_R2.fq.gz

Data processed at 22:43:31 on 2021-07-19


Alignment Stats

Sequence pairs analysed in total 282024119
Paired-end alignments with a unique best hit 252073827
Pairs without alignments under any condition 15405047
Pairs that did not map uniquely 14545245
Genomic sequence context not extractable (edges of chromosomes) 1572

Cytosine Methylation

Total C's analysed 12725697391
Methylated C's in CpG context 463611169
Methylated C's in CHG context 16647730
Methylated C's in CHH context 60569613
Methylated C's in Unknown context 1823716
Unmethylated C's in CpG context 110080298
Unmethylated C's in CHG context 2667544542
Unmethylated C's in CHH context 9407244039
Unmethylated C's in Unknown context 7863118
Percentage methylation (CpG context) 80.8%
Percentage methylation (CHG context) 0.6%
Percentage methylation (CHH context) 0.6%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 126452355 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 125619900 original bottom strand

Deduplication

Alignments analysed 252072255
Unique alignments 204472035
Duplicates removed 47600220
Duplicated alignments were found at 39315820 different positions

Cytosine Methylation after Extraction

Total C's analysed 7618543612
Methylated C's in CpG context 276680859
Methylated C's in CHG context 12192896
Methylated C's in CHH context 44840921
Unmethylated C's in CpG context 65508684
Unmethylated C's in CHG context 1591862125
Unmethylated C's in CHH context 5627458127
Percentage methylation (CpG context) 80.9%
Percentage methylation (CHG context) N/A%
Percentage methylation (CHH context) N/A%

Nucleotide Coverage

Nucleotide Class Counts Sample Counts Genome% in Sample % in Genome fold coverage
A 18714237432 866420001 30.37 29.52 21.599
T 18711654046 868918077 30.36 29.61 21.534
C 11735206046 598683433 19.04 20.40 19.602
G 12469321022 600854940 20.23 20.47 20.753
AC 3007518058 147939993 4.92 5.04 20.329
CA 4296799226 212726856 7.02 7.25 20.199
TC 3480691268 174696228 5.69 5.95 19.924
CT 4052789096 205281198 6.63 6.99 19.743
CC 2750934168 151371046 4.50 5.16 18.173
CG 527932862 29303965 0.86 1.00 18.016
GC 2424287916 124675968 3.96 4.25 19.445
GG 3026231538 152424560 4.95 5.19 19.854
AG 4261799772 205417428 6.97 7.00 20.747
GA 3724598628 175501875 6.09 5.98 21.223
TG 4546351988 213708670 7.43 7.28 21.274
GT 3208235303 148252316 5.24 5.05 21.640
TT 6247210184 289149328 10.21 9.85 21.605
TA 4274480829 191363599 6.99 6.52 22.337
AT 5094241151 226234956 8.33 7.71 22.517
AA 6245475448 286827281 10.21 9.77 21.774

M-Bias Plot