Alignment Stats
| Sequence pairs analysed in total |
305152768 |
| Paired-end alignments with a unique best hit |
274206992 |
| Pairs without alignments under any condition |
16027550 |
| Pairs that did not map uniquely |
14918226 |
| Genomic sequence context not extractable (edges of chromosomes) |
2908 |
|
|
Cytosine Methylation
| Total C's analysed |
13419249214 |
| Methylated C's in CpG context |
482204025 |
| Methylated C's in CHG context |
16826386 |
| Methylated C's in CHH context |
61586618 |
| Methylated C's in Unknown context |
1724412 |
| Unmethylated C's in CpG context |
102457947 |
| Unmethylated C's in CHG context |
2786735890 |
| Unmethylated C's in CHH context |
9969438348 |
| Unmethylated C's in Unknown context |
7961439 |
| Percentage methylation (CpG context) |
82.5% |
| Percentage methylation (CHG context) |
0.6% |
| Percentage methylation (CHH context) |
0.6% |
| Methylated C's in Unknown context |
N/A% |
|
|
Alignment to Individual Bisulfite Strands
| OT |
137670293 |
original top strand |
| CTOT |
0 |
complementary to original top strand |
| CTOB |
0 |
complementary to original bottom strand |
| OB |
136533791 |
original bottom strand |
|
|
Deduplication
| Alignments analysed |
274204084 |
| Unique alignments |
222121564 |
| Duplicates removed |
52082520 |
| Duplicated alignments were found at 42714616 different positions |
|
|
Cytosine Methylation after Extraction
| Total C's analysed |
8216943501 |
| Methylated C's in CpG context |
294645098 |
| Methylated C's in CHG context |
12238913 |
| Methylated C's in CHH context |
45225416 |
| Unmethylated C's in CpG context |
62149950 |
| Unmethylated C's in CHG context |
1702537779 |
| Unmethylated C's in CHH context |
6100146345 |
| Percentage methylation (CpG context) |
82.6% |
| Percentage methylation (CHG context) |
N/A% |
| Percentage methylation (CHH context) |
N/A% |
|
|
Nucleotide Coverage
| Nucleotide Class | Counts Sample | Counts Genome | % in Sample | % in Genome | fold coverage |
|---|
| A | 21266855740 | 866420001 | 31.43 | 29.52 | 24.546 |
|---|
| T | 20486171767 | 868918077 | 30.27 | 29.61 | 23.577 |
|---|
| C | 12429948612 | 598683433 | 18.37 | 20.40 | 20.762 |
|---|
| G | 13489197384 | 600854940 | 19.93 | 20.47 | 22.450 |
|---|
| AC | 3323513148 | 147939993 | 4.95 | 5.04 | 22.465 |
|---|
| CA | 4682726722 | 212726856 | 6.97 | 7.25 | 22.013 |
|---|
| TC | 3681552889 | 174696228 | 5.48 | 5.95 | 21.074 |
|---|
| CT | 4282393191 | 205281198 | 6.38 | 6.99 | 20.861 |
|---|
| CC | 2811314013 | 151371046 | 4.19 | 5.16 | 18.572 |
|---|
| CG | 540035722 | 29303965 | 0.80 | 1.00 | 18.429 |
|---|
| GC | 2539069672 | 124675968 | 3.78 | 4.25 | 20.365 |
|---|
| GG | 3223583767 | 152424560 | 4.80 | 5.19 | 21.149 |
|---|
| AG | 4709733420 | 205417428 | 7.01 | 7.00 | 22.928 |
|---|
| GA | 4157742292 | 175501875 | 6.19 | 5.98 | 23.691 |
|---|
| TG | 4900082575 | 213708670 | 7.30 | 7.28 | 22.929 |
|---|
| GT | 3476198685 | 148252316 | 5.18 | 5.05 | 23.448 |
|---|
| TT | 6825128420 | 289149328 | 10.16 | 9.85 | 23.604 |
|---|
| TA | 4900803155 | 191363599 | 7.30 | 6.52 | 25.610 |
|---|
| AT | 5785175381 | 226234956 | 8.61 | 7.71 | 25.572 |
|---|
| AA | 7329598411 | 286827281 | 10.91 | 9.77 | 25.554 |
|---|
|
|
M-Bias Plot
