Alignment Stats
| Sequence pairs analysed in total |
234273012 |
| Paired-end alignments with a unique best hit |
207664170 |
| Pairs without alignments under any condition |
15489461 |
| Pairs that did not map uniquely |
11119381 |
| Genomic sequence context not extractable (edges of chromosomes) |
1756 |
|
|
Cytosine Methylation
| Total C's analysed |
9489730420 |
| Methylated C's in CpG context |
324328036 |
| Methylated C's in CHG context |
12004853 |
| Methylated C's in CHH context |
45450614 |
| Methylated C's in Unknown context |
1382345 |
| Unmethylated C's in CpG context |
87301907 |
| Unmethylated C's in CHG context |
1929145394 |
| Unmethylated C's in CHH context |
7091499616 |
| Unmethylated C's in Unknown context |
6159462 |
| Percentage methylation (CpG context) |
78.8% |
| Percentage methylation (CHG context) |
0.6% |
| Percentage methylation (CHH context) |
0.6% |
| Methylated C's in Unknown context |
N/A% |
|
|
Alignment to Individual Bisulfite Strands
| OT |
104286064 |
original top strand |
| CTOT |
0 |
complementary to original top strand |
| CTOB |
0 |
complementary to original bottom strand |
| OB |
103376350 |
original bottom strand |
|
|
Deduplication
| Alignments analysed |
207662414 |
| Unique alignments |
142782854 |
| Duplicates removed |
64879560 |
| Duplicated alignments were found at 46107311 different positions |
|
|
Cytosine Methylation after Extraction
| Total C's analysed |
4990486081 |
| Methylated C's in CpG context |
170261790 |
| Methylated C's in CHG context |
7563750 |
| Methylated C's in CHH context |
28905059 |
| Unmethylated C's in CpG context |
45384733 |
| Unmethylated C's in CHG context |
1012670848 |
| Unmethylated C's in CHH context |
3725699901 |
| Percentage methylation (CpG context) |
79.0% |
| Percentage methylation (CHG context) |
N/A% |
| Percentage methylation (CHH context) |
N/A% |
|
|
Nucleotide Coverage
| Nucleotide Class | Counts Sample | Counts Genome | % in Sample | % in Genome | fold coverage |
|---|
| A | 17276294967 | 866420001 | 33.93 | 29.52 | 19.940 |
|---|
| T | 14970486038 | 868918077 | 29.40 | 29.61 | 17.229 |
|---|
| C | 8756003621 | 598683433 | 17.20 | 20.40 | 14.625 |
|---|
| G | 9912231327 | 600854940 | 19.47 | 20.47 | 16.497 |
|---|
| AC | 2532176514 | 147939993 | 5.01 | 5.04 | 17.116 |
|---|
| CA | 3491822370 | 212726856 | 6.91 | 7.25 | 16.415 |
|---|
| TC | 2554951945 | 174696228 | 5.06 | 5.95 | 14.625 |
|---|
| CT | 2939753288 | 205281198 | 5.82 | 6.99 | 14.321 |
|---|
| CC | 1865953792 | 151371046 | 3.69 | 5.16 | 12.327 |
|---|
| CG | 377266053 | 29303965 | 0.75 | 1.00 | 12.874 |
|---|
| GC | 1750829629 | 124675968 | 3.46 | 4.25 | 14.043 |
|---|
| GG | 2321785190 | 152424560 | 4.59 | 5.19 | 15.232 |
|---|
| AG | 3618298998 | 205417428 | 7.16 | 7.00 | 17.614 |
|---|
| GA | 3298159240 | 175501875 | 6.53 | 5.98 | 18.793 |
|---|
| TG | 3510201634 | 213708670 | 6.95 | 7.28 | 16.425 |
|---|
| GT | 2473053231 | 148252316 | 4.89 | 5.05 | 16.681 |
|---|
| TT | 4863540866 | 289149328 | 9.62 | 9.85 | 16.820 |
|---|
| TA | 3909742590 | 191363599 | 7.74 | 6.52 | 20.431 |
|---|
| AT | 4609846502 | 226234956 | 9.12 | 7.71 | 20.376 |
|---|
| AA | 6418015566 | 286827281 | 12.70 | 9.77 | 22.376 |
|---|
|
|
M-Bias Plot
