Bismark

Bismark Processing Report

1_trim/HWVNFDSXY_AGCCAACTAT-ACCAGTCACA_L003_R1.fq.gz and 1_trim/HWVNFDSXY_AGCCAACTAT-ACCAGTCACA_L003_R2.fq.gz

Data processed at 03:42:18 on 2021-07-22


Alignment Stats

Sequence pairs analysed in total 259326758
Paired-end alignments with a unique best hit 231303017
Pairs without alignments under any condition 15066850
Pairs that did not map uniquely 12956891
Genomic sequence context not extractable (edges of chromosomes) 1887

Cytosine Methylation

Total C's analysed 12008741788
Methylated C's in CpG context 436232589
Methylated C's in CHG context 12991759
Methylated C's in CHH context 46671517
Methylated C's in Unknown context 1344895
Unmethylated C's in CpG context 104908627
Unmethylated C's in CHG context 2527903435
Unmethylated C's in CHH context 8880033861
Unmethylated C's in Unknown context 6535160
Percentage methylation (CpG context) 80.6%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 116076475 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 115224655 original bottom strand

Deduplication

Alignments analysed 231301130
Unique alignments 189945904
Duplicates removed 41355226
Duplicated alignments were found at 34595030 different positions

Cytosine Methylation after Extraction

Total C's analysed 7680913595
Methylated C's in CpG context 278383066
Methylated C's in CHG context 9649564
Methylated C's in CHH context 35023494
Unmethylated C's in CpG context 66654155
Unmethylated C's in CHG context 1612770973
Unmethylated C's in CHH context 5678432343
Percentage methylation (CpG context) 80.7%
Percentage methylation (CHG context) N/A%
Percentage methylation (CHH context) N/A%

Nucleotide Coverage

Nucleotide Class Counts Sample Counts Genome% in Sample % in Genome fold coverage
A 17207164405 866420001 29.88 29.52 19.860
T 17523914235 868918077 30.43 29.61 20.168
C 11161650408 598683433 19.38 20.40 18.644
G 11686873606 600854940 20.30 20.47 19.450
AC 2810077296 147939993 4.92 5.04 18.995
CA 4028256601 212726856 7.05 7.25 18.936
TC 3307232590 174696228 5.79 5.95 18.931
CT 3867025930 205281198 6.77 6.99 18.838
CC 2663123268 151371046 4.66 5.16 17.593
CG 502407936 29303965 0.88 1.00 17.145
GC 2314639314 124675968 4.05 4.25 18.565
GG 2847713768 152424560 4.98 5.19 18.683
AG 3961508416 205417428 6.93 7.00 19.285
GA 3429256503 175501875 6.00 5.98 19.540
TG 4274140780 213708670 7.48 7.28 20.000
GT 3015314231 148252316 5.28 5.05 20.339
TT 5857165824 289149328 10.25 9.85 20.257
TA 3934043544 191363599 6.88 6.52 20.558
AT 4684048768 226234956 8.20 7.71 20.704
AA 5657001819 286827281 9.90 9.77 19.723

M-Bias Plot