Alignment Stats
| Sequence pairs analysed in total |
259326758 |
| Paired-end alignments with a unique best hit |
231303017 |
| Pairs without alignments under any condition |
15066850 |
| Pairs that did not map uniquely |
12956891 |
| Genomic sequence context not extractable (edges of chromosomes) |
1887 |
|
|
Cytosine Methylation
| Total C's analysed |
12008741788 |
| Methylated C's in CpG context |
436232589 |
| Methylated C's in CHG context |
12991759 |
| Methylated C's in CHH context |
46671517 |
| Methylated C's in Unknown context |
1344895 |
| Unmethylated C's in CpG context |
104908627 |
| Unmethylated C's in CHG context |
2527903435 |
| Unmethylated C's in CHH context |
8880033861 |
| Unmethylated C's in Unknown context |
6535160 |
| Percentage methylation (CpG context) |
80.6% |
| Percentage methylation (CHG context) |
0.5% |
| Percentage methylation (CHH context) |
0.5% |
| Methylated C's in Unknown context |
N/A% |
|
|
Alignment to Individual Bisulfite Strands
| OT |
116076475 |
original top strand |
| CTOT |
0 |
complementary to original top strand |
| CTOB |
0 |
complementary to original bottom strand |
| OB |
115224655 |
original bottom strand |
|
|
Deduplication
| Alignments analysed |
231301130 |
| Unique alignments |
189945904 |
| Duplicates removed |
41355226 |
| Duplicated alignments were found at 34595030 different positions |
|
|
Cytosine Methylation after Extraction
| Total C's analysed |
7680913595 |
| Methylated C's in CpG context |
278383066 |
| Methylated C's in CHG context |
9649564 |
| Methylated C's in CHH context |
35023494 |
| Unmethylated C's in CpG context |
66654155 |
| Unmethylated C's in CHG context |
1612770973 |
| Unmethylated C's in CHH context |
5678432343 |
| Percentage methylation (CpG context) |
80.7% |
| Percentage methylation (CHG context) |
N/A% |
| Percentage methylation (CHH context) |
N/A% |
|
|
Nucleotide Coverage
| Nucleotide Class | Counts Sample | Counts Genome | % in Sample | % in Genome | fold coverage |
| A | 17207164405 | 866420001 | 29.88 | 29.52 | 19.860 |
| T | 17523914235 | 868918077 | 30.43 | 29.61 | 20.168 |
| C | 11161650408 | 598683433 | 19.38 | 20.40 | 18.644 |
| G | 11686873606 | 600854940 | 20.30 | 20.47 | 19.450 |
| AC | 2810077296 | 147939993 | 4.92 | 5.04 | 18.995 |
| CA | 4028256601 | 212726856 | 7.05 | 7.25 | 18.936 |
| TC | 3307232590 | 174696228 | 5.79 | 5.95 | 18.931 |
| CT | 3867025930 | 205281198 | 6.77 | 6.99 | 18.838 |
| CC | 2663123268 | 151371046 | 4.66 | 5.16 | 17.593 |
| CG | 502407936 | 29303965 | 0.88 | 1.00 | 17.145 |
| GC | 2314639314 | 124675968 | 4.05 | 4.25 | 18.565 |
| GG | 2847713768 | 152424560 | 4.98 | 5.19 | 18.683 |
| AG | 3961508416 | 205417428 | 6.93 | 7.00 | 19.285 |
| GA | 3429256503 | 175501875 | 6.00 | 5.98 | 19.540 |
| TG | 4274140780 | 213708670 | 7.48 | 7.28 | 20.000 |
| GT | 3015314231 | 148252316 | 5.28 | 5.05 | 20.339 |
| TT | 5857165824 | 289149328 | 10.25 | 9.85 | 20.257 |
| TA | 3934043544 | 191363599 | 6.88 | 6.52 | 20.558 |
| AT | 4684048768 | 226234956 | 8.20 | 7.71 | 20.704 |
| AA | 5657001819 | 286827281 | 9.90 | 9.77 | 19.723 |
|
|
M-Bias Plot