Alignment Stats
| Sequence pairs analysed in total |
269899596 |
| Paired-end alignments with a unique best hit |
243550511 |
| Pairs without alignments under any condition |
13595179 |
| Pairs that did not map uniquely |
12753906 |
| Genomic sequence context not extractable (edges of chromosomes) |
2273 |
|
|
Cytosine Methylation
| Total C's analysed |
11639917670 |
| Methylated C's in CpG context |
407494029 |
| Methylated C's in CHG context |
14790044 |
| Methylated C's in CHH context |
54296961 |
| Methylated C's in Unknown context |
1555111 |
| Unmethylated C's in CpG context |
91941207 |
| Unmethylated C's in CHG context |
2405475415 |
| Unmethylated C's in CHH context |
8665920014 |
| Unmethylated C's in Unknown context |
7016709 |
| Percentage methylation (CpG context) |
81.6% |
| Percentage methylation (CHG context) |
0.6% |
| Percentage methylation (CHH context) |
0.6% |
| Methylated C's in Unknown context |
N/A% |
|
|
Alignment to Individual Bisulfite Strands
| OT |
122178436 |
original top strand |
| CTOT |
0 |
complementary to original top strand |
| CTOB |
0 |
complementary to original bottom strand |
| OB |
121369802 |
original bottom strand |
|
|
Deduplication
| Alignments analysed |
243548238 |
| Unique alignments |
193915929 |
| Duplicates removed |
49632309 |
| Duplicated alignments were found at 40093609 different positions |
|
|
Cytosine Methylation after Extraction
| Total C's analysed |
7001316664 |
| Methylated C's in CpG context |
245039006 |
| Methylated C's in CHG context |
10660515 |
| Methylated C's in CHH context |
39550043 |
| Unmethylated C's in CpG context |
54882536 |
| Unmethylated C's in CHG context |
1444279324 |
| Unmethylated C's in CHH context |
5206905240 |
| Percentage methylation (CpG context) |
81.7% |
| Percentage methylation (CHG context) |
N/A% |
| Percentage methylation (CHH context) |
N/A% |
|
|
Nucleotide Coverage
| Nucleotide Class | Counts Sample | Counts Genome | % in Sample | % in Genome | fold coverage |
|---|
| A | 19345576087 | 866420001 | 32.25 | 29.52 | 22.328 |
|---|
| T | 18002959047 | 868918077 | 30.01 | 29.61 | 20.719 |
|---|
| C | 10775076912 | 598683433 | 17.96 | 20.40 | 17.998 |
|---|
| G | 11871389232 | 600854940 | 19.79 | 20.47 | 19.757 |
|---|
| AC | 2967134178 | 147939993 | 4.98 | 5.04 | 20.056 |
|---|
| CA | 4139678714 | 212726856 | 6.95 | 7.25 | 19.460 |
|---|
| TC | 3167511477 | 174696228 | 5.32 | 5.95 | 18.132 |
|---|
| CT | 3690727888 | 205281198 | 6.20 | 6.99 | 17.979 |
|---|
| CC | 2385520804 | 151371046 | 4.01 | 5.16 | 15.759 |
|---|
| CG | 460226095 | 29303965 | 0.77 | 1.00 | 15.705 |
|---|
| GC | 2190394783 | 124675968 | 3.68 | 4.25 | 17.569 |
|---|
| GG | 2819254803 | 152424560 | 4.73 | 5.19 | 18.496 |
|---|
| AG | 4218969608 | 205417428 | 7.08 | 7.00 | 20.539 |
|---|
| GA | 3746363978 | 175501875 | 6.29 | 5.98 | 21.347 |
|---|
| TG | 4271548345 | 213708670 | 7.17 | 7.28 | 19.988 |
|---|
| GT | 3033434687 | 148252316 | 5.09 | 5.05 | 20.461 |
|---|
| TT | 5962489760 | 289149328 | 10.01 | 9.85 | 20.621 |
|---|
| TA | 4444153455 | 191363599 | 7.46 | 6.52 | 23.224 |
|---|
| AT | 5213425297 | 226234956 | 8.75 | 7.71 | 23.044 |
|---|
| AA | 6837223310 | 286827281 | 11.48 | 9.77 | 23.837 |
|---|
|
|
M-Bias Plot
