FastQCFastQC Report
Sun 6 Jun 2021
HWVNFDSXY_TCAGATTCAT-ACTGTGGTTG_L004_R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWVNFDSXY_TCAGATTCAT-ACTGTGGTTG_L004_R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences346658058
Sequences flagged as poor quality0
Sequence length151
%GC24

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCAACCACAGTGTAGATC13102170.3779565972183459Illumina Single End PCR Primer 1 (96% over 33bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)