FastQCFastQC Report
Tue 8 Jun 2021
HWVNFDSXY_CCACCGATAT-ACCTCTATCG_L003_R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWVNFDSXY_CCACCGATAT-ACCTCTATCG_L003_R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences303083886
Sequences flagged as poor quality0
Sequence length151
%GC27

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCGATAGAGGTGTAGATC87258082.879007562942492Illumina Single End PCR Primer 1 (97% over 37bp)
GAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCGATAGAGGTGTAGA9255470.3053765121646883Illumina Single End PCR Primer 1 (97% over 37bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5341180.1762277787344986No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)