FastQCFastQC Report
Tue 8 Jun 2021
HWVNFDSXY_CCACCGATAT-ACCTCTATCG_L003_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWVNFDSXY_CCACCGATAT-ACCTCTATCG_L003_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences303083886
Sequences flagged as poor quality0
Sequence length151
%GC25

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCACCGATATCTCGTA75696942.497557392411156TruSeq Adapter, Index 23 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCACCGATATCGCGTA13367840.44106072996569673TruSeq Adapter, Index 23 (97% over 37bp)
TCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCACCGATATCTCG7016850.23151511261802946TruSeq Adapter, Index 23 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)