FastQCFastQC Report
Tue 8 Jun 2021
HWVNFDSXY_AGTATCTGAT-ACACTTGATG_L003_R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWVNFDSXY_AGTATCTGAT-ACACTTGATG_L003_R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences242430534
Sequences flagged as poor quality0
Sequence length151
%GC25

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCATCAAGTGTGTAGATC32636561.3462231618068374Illumina Single End PCR Primer 1 (96% over 33bp)
GAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCATCAAGTGTGTAGA2566140.10585052788771236Illumina Single End PCR Primer 1 (96% over 33bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2436050.10048445465206952No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)