FastQCFastQC Report
Sun 14 Aug 2016
V9P_input.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameV9P_input.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38836698
Sequences flagged as poor quality0
Sequence length36
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACCCG14650.013.1022135
CAGATCG26000.011.71489125
CGGTGAC17200.011.6829512
GACCCGG17250.011.6488036
CCGGGTC16150.010.86433114
CGGGTCA16400.010.60727415
ATGCCGA22400.010.1761914
GTCACCG17850.09.82982318
GCGGTGA21000.09.5266141
CGGTTCA25200.09.1326561
TGCCGAG26650.09.11606615
GATCGGA35650.09.04902627
TCGGAAG36750.09.0231929
CGCAGAT18700.08.98538323
ATCGGAA36300.08.96972828
AATGCCG25500.08.82146513
GAGCGGT20400.08.67914529
ACCGATC6900.08.47912722
ACCGCAG20000.08.39897421
CCGCAGA20550.08.17601722