FastQCFastQC Report
Sun 14 Aug 2016
V9P_H3K4me1_ChIPSeq.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameV9P_H3K4me1_ChIPSeq.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21067328
Sequences flagged as poor quality0
Sequence length152
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCG379230.1800085896037694Illumina Paired End PCR Primer 2 (100% over 31bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA56100.037.385765145-146
CGGTTCG52350.024.8971470-74
GCGGTTA98500.023.98972770-74
AGCGGTA98800.023.82824970-74
TCGGAAC128450.023.52073570-74
CGCCGTA37100.022.806013140-144
AGCGGTC132750.022.65990870-74
ATCGGAC138000.022.30551170-74
TGCCGAC90150.021.72708570-74
CGGTTCC150800.021.32199570-74
TAGATCT428750.021.095175145-146
ATCGGAT100300.020.76527870-74
ATGCCGC100450.020.5017970-74
TCGGAAA149900.020.30107970-74
GATCGGC142900.020.18224170-74
TCGGAAT100300.020.08134770-74
TGCCGAA81700.020.00832270-74
CGGTTCT117300.019.90841370-74
GATCGGT97100.019.70598470-74
GCGGTTG88050.019.62623870-74
Produced by FastQC (version 0.11.3)