##FastQC	0.11.3
>>Basic Statistics	pass
#Measure	Value
Filename	V9M_input.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	11493872
Sequences flagged as poor quality	0
Sequence length	54
%GC	42
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.811625012006395	36.0	34.0	37.0	29.0	38.0
2	31.33200004315343	35.0	30.0	37.0	20.0	38.0
3	31.84907984010958	35.0	30.0	37.0	20.0	38.0
4	31.765898819823295	35.0	30.0	37.0	21.0	38.0
5	31.859882118053864	35.0	30.0	37.0	21.0	38.0
6	32.51242174960709	35.0	32.0	37.0	24.0	38.0
7	32.29811111520991	35.0	31.0	37.0	23.0	38.0
8	32.28973499965895	35.0	31.0	37.0	23.0	38.0
9	32.38573911385128	35.0	31.0	37.0	24.0	38.0
10	32.45131649282331	35.0	32.0	37.0	24.0	38.0
11	33.37436957711031	36.0	32.0	38.0	28.0	38.0
12	33.277569821553605	36.0	32.0	37.0	27.0	38.0
13	33.24934756538093	36.0	32.0	37.0	27.0	38.0
14	33.18636791848735	36.0	32.0	37.0	27.0	38.0
15	33.13493416317843	36.0	32.0	37.0	27.0	38.0
16	32.822269466721046	35.0	32.0	37.0	26.0	38.0
17	32.68271014328331	35.0	32.0	37.0	26.0	38.0
18	32.50523548548305	35.0	32.0	37.0	25.0	38.0
19	32.588342553318846	35.0	32.0	37.0	25.0	38.0
20	32.39401065193696	35.0	31.0	37.0	25.0	38.0
21	30.458252623658936	34.0	29.0	36.0	19.0	38.0
22	27.351666348816135	33.0	20.0	36.0	7.0	38.0
23	30.457683450798825	34.0	29.0	36.0	20.0	38.0
24	30.971268254944896	34.0	30.0	36.0	21.0	38.0
25	31.01855197273817	34.0	30.0	36.0	21.0	38.0
26	30.08515850881235	34.0	28.0	36.0	16.0	38.0
27	30.614065999690965	34.0	29.0	36.0	20.0	38.0
28	30.71246060509461	34.0	29.0	36.0	20.0	38.0
29	30.54494734237514	34.0	29.0	36.0	20.0	38.0
30	30.210766745966893	34.0	29.0	36.0	18.0	38.0
31	30.574617239516847	34.0	29.0	36.0	20.0	38.0
32	29.769999178692785	33.0	28.0	36.0	13.0	38.0
33	30.06770146735582	34.0	29.0	36.0	13.0	38.0
34	29.15610666275038	33.0	28.0	36.0	11.0	37.0
35	29.95936539053158	34.0	29.0	36.0	13.0	37.0
36	27.47325331272177	32.0	24.0	36.0	6.0	37.0
37	28.046854619574674	32.0	25.0	35.0	7.0	37.0
38	28.909672301901395	33.0	28.0	35.0	8.0	37.0
39	27.93774160700589	32.0	25.0	35.0	2.0	37.0
40	28.616775704479743	33.0	28.0	35.0	2.0	37.0
41	25.85195267530385	31.0	19.0	35.0	2.0	37.0
42	27.733231847370494	32.0	25.0	35.0	2.0	37.0
43	26.06622850854786	31.0	20.0	35.0	2.0	37.0
44	26.26317171445793	31.0	21.0	35.0	2.0	37.0
45	26.18570861064052	31.0	21.0	35.0	2.0	37.0
46	27.36515196967567	32.0	25.0	35.0	2.0	37.0
47	26.844665574838487	32.0	25.0	35.0	2.0	37.0
48	25.930891870033005	31.0	21.0	35.0	2.0	37.0
49	26.361800096608	32.0	23.0	35.0	2.0	37.0
50	25.82754575655619	31.0	21.0	35.0	2.0	36.0
51	26.42947581111048	32.0	24.0	36.0	2.0	37.0
52	25.409318026162115	32.0	20.0	35.0	2.0	37.0
53	24.842762821788863	31.0	17.0	35.0	2.0	37.0
54	23.69516216989366	30.0	2.0	35.0	2.0	36.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1	1	1.3596886891936393
1	2	1.3640129478225198
1	3	2.221774755725612
1	4	2.182221866973766
1	5	2.147888878565645
1	6	1.8077120972700698
1	7	1.9259602772881301
1	8	1.8381661330293575
1	9	1.6909472607482101
1	10	1.7360006441384073
1	11	1.2026523753177543
1	12	1.1856071820299903
1	13	1.2185730959755645
1	14	1.0895998550206372
1	15	1.0984430955045568
1	16	1.4712953633412766
1	17	1.5794180789086099
1	18	1.5572381074483346
1	19	1.5678326733346921
1	20	1.7048839828993323
1	21	3.9635127010495417
1	22	6.97635638293055
1	23	3.531035483788745
1	24	2.920073805799607
1	25	2.911603790505758
1	26	3.8186564131784415
1	27	3.1957830524828985
1	28	2.9968341648626406
1	29	3.0602836238135502
1	30	3.569672759893866
1	31	3.035603891030103
1	32	3.591525771407909
1	33	3.3418566268433665
1	34	4.15260010211103
1	35	3.3198905776795264
1	36	5.915653296114144
1	37	5.038168644108062
1	38	4.052674052500649
1	39	4.852943493178845
1	40	3.9682719329381193
1	41	6.548506234055154
1	42	4.4915966211695775
1	43	6.076774476408236
1	44	5.972658822595104
1	45	5.521171731365417
1	46	4.386224066818269
1	47	4.519277338248656
1	48	5.272774089702345
1	49	4.720290988597782
1	50	5.069770759363259
1	51	4.172746278320112
1	52	4.604820131921709
1	53	4.832478342133143
1	54	4.661845232682076
2	1	1.1491807405373962
2	2	1.295135750472138
2	3	1.6283960372044746
2	4	1.9480204701802997
2	5	1.582627894614241
2	6	1.4439328094029875
2	7	1.723577038496483
2	8	1.6052370710914836
2	9	1.4437791901092254
2	10	1.4364695613765477
2	11	0.9579437856956332
2	12	0.8850177750348465
2	13	0.918354556410307
2	14	0.8644995106282565
2	15	0.9548345150607744
2	16	1.1372966758915837
2	17	1.2165945515799308
2	18	1.3878875753336715
2	19	1.2479621499117428
2	20	1.3253232560377128
2	21	3.0034160460593604
2	22	5.911167232712021
2	23	1.9022791110480668
2	24	2.272639683852031
2	25	2.4257242758060826
2	26	2.86478405854486
2	27	-2.450392089570528
2	28	2.142731141287328
2	29	2.136742494507171
2	30	2.2194988201784334
2	31	0.9992649807130505
2	32	-3.777480034070617
2	33	1.53855875236302
2	34	-2.800303302642284
2	35	1.1918642259767545
2	36	-1.0401442507405463
2	37	2.343179853241878
2	38	2.1402730086997472
2	39	1.7692550865019285
2	40	1.9529765121571607
2	41	5.159103773875525
2	42	2.262288545486122
2	43	4.3732918096124465
2	44	4.305097584300196
2	45	3.7941175576192663
2	46	3.4502206355417258
2	47	3.4310594164745822
2	48	3.6195429358264484
2	49	3.6729149178219664
2	50	3.1795277044852384
2	51	3.224576085702722
2	52	2.9125740165141245
2	53	2.431867199825323
2	54	2.4118585274182145
3	1	1.193863174414048
3	2	1.4056710732871416
3	3	1.8598980219957468
3	4	1.570199932809171
3	5	1.7331506453906513
3	6	1.5025354401695523
3	7	1.6518086646018624
3	8	1.6063507099259304
3	9	1.4948576734662424
3	10	1.5181818811119498
3	11	0.9904874408605195
3	12	0.9368525876682199
3	13	0.8468870823683119
3	14	0.9544897101833172
3	15	1.1157189063910735
3	16	1.3071173167526453
3	17	1.2879193743093467
3	18	1.4623312664731785
3	19	1.1925782707251429
3	20	1.1178834330426852
3	21	2.9658872893725494
3	22	5.7010993329955575
3	23	2.0314739917847398
3	24	2.2802935463330876
3	25	1.9204581997693921
3	26	-3.3195597330741293
3	27	2.0046181165946813
3	28	1.8064142811589363
3	29	1.9520712893518883
3	30	2.3436075780905803
3	31	0.2519876426766352
3	32	0.3350702325233499
3	33	-4.787941248069789
3	34	-7.8359655537066075
3	35	0.48506794490721106
3	36	3.720277189871961
3	37	2.6088857723752703
3	38	2.0197989658365145
3	39	2.177611083905191
3	40	2.2807332515780807
3	41	5.805947454486496
3	42	3.9891544187235937
3	43	5.332270091507148
3	44	5.142250202545693
3	45	4.343092274754007
3	46	2.6307267077042766
3	47	2.715506983356793
3	48	-2.8643798172134005
3	49	2.485544815235169
3	50	2.672804129258303
3	51	2.0669129145783103
3	52	1.6109930956454797
3	53	1.9285340418606118
3	54	2.190508478217218
4	1	1.1614439352280144
4	2	1.875874919038484
4	3	0.43209723564304525
4	4	1.8667196258144365
4	5	1.9483019620224908
4	6	1.7651197026861567
4	7	1.8246801287861025
4	8	1.8762062708075256
4	9	1.7083096888038085
4	10	1.6692700580297384
4	11	1.278668260694488
4	12	1.253372438187462
4	13	1.4575828299326048
4	14	1.3965460479190455
4	15	1.4423096871221972
4	16	-1.811807521518098
4	17	-1.5948957201590588
4	18	-1.475510379242344
4	19	-1.6980097205767457
4	20	-4.456397053279318
4	21	-27.901028798281114
4	22	1.9981875679071202
4	23	-1.5747055199672957
4	24	-4.384482833349086
4	25	-1.8023677464570085
4	26	-13.674450643312763
4	27	-8.119618820694114
4	28	-2.448975530718073
4	29	-2.1833021840304596
4	30	-1.7093317937079036
4	31	-2.8710736676081083
4	32	-2.8877355471415704
4	33	-13.67320019566883
4	34	-17.038288312005356
4	35	-2.409410641486037
4	36	1.7549881976761839
4	37	0.6750695632568586
4	38	0.4155577708973439
4	39	-0.1759263398034534
4	40	0.3566497352839164
4	41	5.499024202333715
4	42	3.535889947634626
4	43	4.895904423300067
4	44	4.51319655961149
4	45	3.554615340586853
4	46	1.283419737197935
4	47	0.4478587659913309
4	48	-9.28635694212759
4	49	0.708639728500458
4	50	-0.26309699206218085
4	51	-0.3824410931797324
4	52	-0.9608310080254441
4	53	-0.5060322082173982
4	54	0.2032579812427393
5	1	1.9161734631092173
5	2	2.667332743508215
5	3	2.971776805624806
5	4	2.8581318597354475
5	5	2.9588998110086777
5	6	2.575686284108123
5	7	2.6381675705762966
5	8	2.664987673453215
5	9	2.5747725113195727
5	10	2.4850613274270543
5	11	1.7876121477936735
5	12	1.8689067307574376
5	13	1.8113389696014153
5	14	1.796150560117944
5	15	1.9026834565195756
5	16	-1.281490827525083
5	17	-1.173382385679787
5	18	-0.9836969530299235
5	19	-1.0857328608656402
5	20	-1.305693166313226
5	21	-27.901028798281114
5	22	3.550385809507432
5	23	0.169835556307941
5	24	-0.34448371864161587
5	25	-0.33217706094944077
5	26	-2.0181971544169954
5	27	-13.072175207488606
5	28	-2.052388071264886
5	29	-2.1399762146067793
5	30	-7.344393113701756
5	31	-3.1045419450332794
5	32	-3.1073536346661967
5	33	-18.413982534627323
5	34	-19.078929389835906
5	35	-2.499491349562767
5	36	0.4549449892500128
5	37	0.06992982000732084
5	38	-0.7040161099783298
5	39	-2.2032015170302195
5	40	-0.1682969990693124
5	41	4.575898422960563
5	42	4.0288490613620205
5	43	5.519295813119349
5	44	5.3100174872412715
5	45	4.352597912171589
5	46	3.984539584354902
5	47	3.9897455174650887
5	48	3.754898420830628
5	49	3.5960191751386823
5	50	2.9983632672140885
5	51	3.628281886561666
5	52	2.964003799337597
5	53	2.597289723445531
5	54	2.979486984439397
6	1	1.1083536880433158
6	2	1.8614229011647332
6	3	0.22269004378357948
6	4	1.8237350910192127
6	5	1.9574228153562174
6	6	1.903684486548606
6	7	2.0398773623329625
6	8	2.0566688858732753
6	9	1.950520490098775
6	10	1.797267097278116
6	11	1.5394760032133163
6	12	1.4538945593669226
6	13	1.5242696958787008
6	14	1.5615325002548701
6	15	1.542709638961469
6	16	-1.8058835417131718
6	17	-1.5537981929559344
6	18	-1.4100130452656465
6	19	-1.6419118027207737
6	20	-2.09030061763114
6	21	-27.901028798281114
6	22	2.232780893208915
6	23	-3.356357856985589
6	24	-1.8514182176047562
6	25	-1.1891407263348412
6	26	-7.772285381656271
6	27	-2.7476123825724237
6	28	-2.5209121737817064
6	29	-3.16055519669667
6	30	-11.709285892422944
6	31	-3.702546845431417
6	32	-3.4167594588612893
6	33	-19.618343071305965
6	34	-19.586441830192967
6	35	-3.238241560862992
6	36	-0.3125424987657297
6	37	-0.941478633652121
6	38	-1.9577529693689293
6	39	-7.913520364073598
6	40	-1.3079387193249872
6	41	3.613233587982844
6	42	1.876089373620907
6	43	0.963114726737416
6	44	3.450722841320058
6	45	3.1989967807398365
6	46	1.9882112715761124
6	47	1.9300464721209245
6	48	-2.6450841690430167
6	49	2.430221159059297
6	50	1.8193968786914034
6	51	1.5924248151895668
6	52	0.6979293743414203
6	53	1.6669106364728385
6	54	0.5213177553005366
7	1	-0.6819286922890555
7	2	-2.980949107861001
7	3	-5.580885314111647
7	4	-0.40131908208103084
7	5	-0.7748718935628744
7	6	0.18590208833845168
7	7	0.43543748281518546
7	8	0.4006822976653801
7	9	0.12475863399018294
7	10	0.37860455448820574
7	11	0.8784934902842565
7	12	0.7414012130666947
7	13	0.8421570135719421
7	14	0.7698927258208101
7	15	0.9923534023477814
7	16	-0.18073350948696998
7	17	-0.08186779867060778
7	18	0.05628867058054965
7	19	-0.12760239200359536
7	20	-0.17650445835348094
7	21	-8.692742376304082
7	22	3.8340691336557384
7	23	0.6141494829814924
7	24	0.3215804742206423
7	25	0.30547010617750914
7	26	-6.962638096090341
7	27	0.5209099316877754
7	28	0.5713634235320875
7	29	0.49940362931781834
7	30	0.7166090941372438
7	31	-1.9415866959820605
7	32	-2.107421108206921
7	33	-1.8330866914270878
7	34	-18.022871449335312
7	35	-1.4882342130091608
7	36	1.7321076973715002
7	37	0.1209463887276101
7	38	0.4328326626222889
7	39	0.1356217744190964
7	40	0.38708235438823735
7	41	4.736689764544295
7	42	3.2446351394248403
7	43	4.811796942614727
7	44	4.379516098580378
7	45	3.605747349005231
7	46	1.500002173314659
7	47	1.2068488168373328
7	48	-6.111355877588572
7	49	1.3111582292333708
7	50	0.2738163193710612
7	51	0.007914505081391354
7	52	-0.23507025978849327
7	53	-0.2521676802209285
7	54	-1.0416565512305773
8	1	1.2681132860299158
8	2	1.2715784600910816
8	3	1.9561784959425523
8	4	1.882711269712022
8	5	1.9851207442784258
8	6	1.6465354180627543
8	7	1.6402582799514
8	8	1.6712890108443865
8	9	1.6326969196119308
8	10	1.6142413652904963
8	11	1.124687824695009
8	12	0.9448423962259582
8	13	1.091677894449532
8	14	1.0169267364415546
8	15	1.0325289413756025
8	16	0.3878664618999821
8	17	0.448713375400402
8	18	0.6635738249158081
8	19	0.43156716941089
8	20	0.34208371833507556
8	21	-5.429562599861448
8	22	5.091754836563638
8	23	1.4396919122535543
8	24	1.206335254873629
8	25	1.2159041056087219
8	26	1.3499968759954655
8	27	-0.9019892923838384
8	28	0.9036791332641982
8	29	1.1254053192579008
8	30	-2.7917199135214794
8	31	1.7627908801337249
8	32	1.815680511527301
8	33	2.2019360284415654
8	34	1.622383023671432
8	35	1.8138647183158234
8	36	4.875880655654733
8	37	3.5072805321048826
8	38	3.130673862686667
8	39	3.088603355337458
8	40	3.28614806139106
8	41	4.882964200542116
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118	51	2.7288839647823195
118	52	2.435943302313241
118	53	2.920389416830073
118	54	2.312668301183926
119	1	1.1329637144844469
119	2	1.4493750387881725
119	3	1.5827182900862624
119	4	1.735743478049681
119	5	1.7541389726729975
119	6	1.5040840573398384
119	7	1.5633598406650115
119	8	1.5187477979049184
119	9	1.2628368550483273
119	10	1.4452980088522978
119	11	0.7726344353916872
119	12	0.8027546015975275
119	13	0.9046582247021746
119	14	0.9568164106668888
119	15	0.9829672633241486
119	16	1.2676381807702057
119	17	1.128093423761733
119	18	1.4378352847178846
119	19	1.181781033695657
119	20	1.2555907116365859
119	21	3.4861017274839945
119	22	6.189474987788561
119	23	2.9608378303272183
119	24	2.3031975248452845
119	25	2.609141434323682
119	26	3.020080471956053
119	27	2.7422641420692493
119	28	2.7539104690330234
119	29	2.6440547314369987
119	30	3.010525520487203
119	31	2.5075029748534057
119	32	2.8489202459842176
119	33	3.100978102719843
119	34	3.468504950031793
119	35	2.781747246660796
119	36	5.045402609874543
119	37	3.5383520877964934
119	38	3.2533645334934143
119	39	3.1204506786037776
119	40	3.428783960996295
119	41	5.264071389436467
119	42	3.798675205801942
119	43	5.291607345245168
119	44	5.111132741813702
119	45	3.9325586908346466
119	46	3.297939964325817
119	47	3.6473256068308757
119	48	3.8836154409824317
119	49	3.0425012920139025
119	50	3.3420037317095286
119	51	2.5441027916521897
119	52	2.83427262232626
119	53	2.7593778487829894
119	54	2.716766745396111
120	1	1.3197741730033385
120	2	1.5865132528546262
120	3	2.074635827412756
120	4	1.9596273904721677
120	5	1.9065350424703311
120	6	1.6440944423569235
120	7	1.7973466683669166
120	8	1.751412972493835
120	9	1.5734638418332239
120	10	1.5325573352191526
120	11	1.109280305784246
120	12	1.0709648062787736
120	13	1.0472179994645572
120	14	1.1469576467493212
120	15	1.1665203486271096
120	16	1.3690171044185107
120	17	1.3871427868547528
120	18	1.5649233482683087
120	19	1.474474905778365
120	20	1.362324823936163
120	21	3.538266128632042
120	22	6.230570041807969
120	23	3.1978972271220663
120	24	2.156849153104563
120	25	2.599225414865046
120	26	2.863318639038024
120	27	2.3460605885371173
120	28	2.671635433566003
120	29	2.7183100401697224
120	30	2.921386162659413
120	31	2.4153236154422295
120	32	2.7113054921826674
120	33	2.9348267734084175
120	34	3.1885480929853927
120	35	2.7356277021591033
120	36	5.047788284908528
120	37	3.9816827693968335
120	38	3.389975234874168
120	39	2.593903075273346
120	40	3.479742981937786
120	41	5.186300259109224
120	42	3.742664794694864
120	43	4.775021433856832
120	44	4.921191776422454
120	45	4.236946855363847
120	46	3.3361793335717245
120	47	3.5784395963380504
120	48	3.5939113472449833
120	49	3.6070453577510904
120	50	3.1257604389183946
120	51	2.653376135093147
120	52	2.960514635358212
120	53	3.1583660143467895
120	54	2.9751094819980572
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	374129.0
3	5320.0
4	1662.0
5	2437.0
6	14935.0
7	19595.0
8	20444.0
9	30688.0
10	33095.0
11	34227.0
12	37025.0
13	41867.0
14	47591.0
15	55448.0
16	65514.0
17	75366.0
18	83936.0
19	95960.0
20	111128.0
21	128031.0
22	150022.0
23	181258.0
24	221338.0
25	277333.0
26	348909.0
27	437728.0
28	542027.0
29	653815.0
30	770702.0
31	886097.0
32	999268.0
33	1104575.0
34	1166799.0
35	1109363.0
36	926467.0
37	439765.0
38	8.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	17.908238604338354	57.7410524420135	5.33872966646529	19.011979287182854
2	29.150655236111906	40.91465434798647	16.388750457635165	13.545939958266457
3	21.580073277308117	33.039832007873414	26.244471836818782	19.135622877999687
4	22.38638119512728	29.95939053436475	27.145873905677735	20.508354364830232
5	22.248533827416907	31.442867990873747	26.76921232461959	19.539385857089762
6	22.139997730964815	32.29214663257082	25.488068772646848	20.07978686381752
7	22.31599586283891	32.400978538824866	26.338669858164423	18.944355740171805
8	20.589449752006985	31.00623532261365	29.03748188599977	19.366833039379593
9	20.01213001897806	30.240097290721717	29.999776401170752	19.747996289129468
10	20.30331643708199	30.910882852261	28.18710076004855	20.59869995060846
11	20.5284433304982	31.05747132036967	27.93953160431924	20.474553744812887
12	20.59467530037101	30.66121599263051	27.97859450112055	20.765514205877935
13	20.395423020344413	30.61498531216819	28.42391264629105	20.565679021196345
14	20.888962628684908	30.42169699341449	27.86722208161586	20.822118296284742
15	20.968747520417836	30.592510513428373	27.68396933600792	20.754772630145872
16	20.894473159262606	30.458560874873147	27.871103836896737	20.775862128967507
17	20.858445265442317	30.33097984734822	28.084260900069186	20.726313987140276
18	20.815283135221968	29.874701928123095	28.30626615643536	21.00374878021958
19	20.99382175127755	29.59138574015789	28.47227635734938	20.942516151215187
20	21.183634531033377	29.60490558634259	28.21789246148044	20.993567421143595
21	21.222436600285018	29.194369955150602	28.176776551950795	21.40641689261359
22	21.733945025966193	27.78304632327544	29.0244471144853	21.458561536273066
23	20.971167523413538	28.93421083655518	28.679209627628982	21.415412012402303
24	21.197773107266023	29.198660189354964	28.412257915376234	21.19130878800278
25	21.435051250649177	29.34270582225722	28.00568657696806	21.216556350125547
26	21.351007296871487	29.227082316591353	27.929104568533575	21.49280581800359
27	21.322148531633438	29.708303160205098	28.02500176532935	20.944546542832114
28	21.067575340256152	29.66928777795236	28.292673070163122	20.97046381162837
29	21.184850874329463	29.359335004829536	28.589737591824044	20.86607652901696
30	21.23398391048622	28.577794847945814	28.388097719881618	21.800123521686345
31	21.406399460324224	29.74556326918766	28.013024412114024	20.835012858374093
32	21.495001833315303	29.48145940775343	28.050150594689537	20.973388164241726
33	21.11685626006687	29.505918835341095	28.419295534758106	20.957929369833927
34	21.50209409536377	28.74554337399754	28.648803121097966	21.10355940954073
35	21.53906166001665	29.155395732847545	28.366071682180667	20.939470924955142
36	21.75376730985271	27.81281542260415	28.464580335190647	21.9688369323525
37	21.364211180886304	27.953461889172697	28.25729827780442	22.42502865213657
38	21.32950294158368	29.63478810324771	28.241590711006666	20.794118244161943
39	21.626003359016583	28.983571754300584	28.275929521268722	21.11449536541411
40	21.40782509020047	29.34474241558103	28.318987375336	20.9284451188825
41	21.901645875967972	27.121062163118655	28.537951845079917	22.439340115833456
42	21.2318518822225	29.624820405275614	28.356600318756104	20.786727393745785
43	21.447910771696467	27.034742429223297	28.76813180201446	22.749214997065774
44	21.650796099548067	27.698205101378992	28.586819574648615	22.064179224424326
45	21.837589720095117	28.227737417370246	28.30087483910378	21.633798023430852
46	21.81917025475257	29.751065593154348	27.97463874751887	20.45512540457421
47	21.575031619995976	29.528802028918815	28.20707091654548	20.68909543453973
48	21.8379240829776	28.26350997816112	28.466945502673862	21.431620436187412
49	22.212206811240254	29.277173398525015	28.007030835976387	20.503588954258344
50	22.377743923901797	29.51748793759148	27.636763632342713	20.468004506164007
51	22.29560465183986	29.678049015907522	27.679071415588158	20.34727491666446
52	22.47168959423667	29.217322674880158	27.908524837212926	20.402462893670247
53	22.38437969328675	29.075063756621383	27.916016868966707	20.624539681125157
54	23.280566642788713	28.107646461861858	27.763247870197667	20.848539025151762
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	421.0
1	359.0
2	297.0
3	339.5
4	382.0
5	481.5
6	813.0
7	1045.0
8	1456.5
9	1868.0
10	2824.5
11	3781.0
12	5752.5
13	7724.0
14	11757.0
15	15790.0
16	23064.0
17	30338.0
18	42106.0
19	71261.5
20	88649.0
21	113151.0
22	137653.0
23	169350.0
24	201047.0
25	239044.0
26	277041.0
27	320149.5
28	363258.0
29	408160.5
30	453063.0
31	499374.5
32	589046.0
33	632406.0
34	671246.0
35	710086.0
36	739860.5
37	769635.0
38	783741.5
39	797848.0
40	793120.0
41	788392.0
42	768526.5
43	748661.0
44	719298.0
45	657576.5
46	625218.0
47	592554.0
48	559890.0
49	530981.5
50	502073.0
51	478728.5
52	455384.0
53	433847.5
54	412311.0
55	389430.0
56	340681.0
57	314813.0
58	286106.5
59	257400.0
60	228381.0
61	199362.0
62	173262.0
63	147162.0
64	125649.5
65	104137.0
66	88227.5
67	72318.0
68	60919.0
69	41361.0
70	33202.0
71	27970.5
72	22739.0
73	19045.0
74	15351.0
75	12978.5
76	10606.0
77	9011.5
78	7417.0
79	6411.0
80	5405.0
81	4624.5
82	3277.0
83	2710.0
84	2389.0
85	2068.0
86	1764.5
87	1461.0
88	1270.5
89	1080.0
90	952.5
91	825.0
92	699.5
93	574.0
94	493.0
95	373.5
96	335.0
97	261.0
98	187.0
99	122.5
100	58.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.28498664331741297
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	6.351210453709594E-4
10	8.700288292752868E-6
11	0.0
12	2.6100864878258608E-5
13	3.480115317101147E-5
14	2.6100864878258608E-5
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	3.2191066683185613E-4
21	1.7329582233036873
22	7.134236400057352E-4
23	0.0017487579468433267
24	7.830259463477582E-5
25	0.03792455666810975
26	0.025404841814838375
27	0.07624062630939339
28	0.10638712524378208
29	0.13514157805133029
30	0.08046896641967129
31	0.02199432880407925
32	0.03270438369245803
33	0.13058262698592782
34	0.01979315586601278
35	0.14538181737190045
36	0.07211668965862854
37	0.15911087229786444
38	0.14695656955288872
39	0.10388144221546926
40	0.2510381183990913
41	0.1648617628593741
42	0.26589821080311316
43	0.2241368269978994
44	0.29197297481649354
45	0.30122138127168985
46	0.40857423851596747
47	0.36077485463558323
48	0.33195079952169293
49	0.3805506099250105
50	0.3886070768840996
51	0.48786866601611717
52	0.4344488958986145
53	0.4176312386287232
54	0.49194040093712543
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
54	1.1493872E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	98.08538563651145
#Duplication Level	Percentage of deduplicated	Percentage of total
1	99.1252348152684	97.22736883165352
2	0.5106730621158533	1.0017912846362327
3	0.15041045690724375	0.44259203008532677
4	0.0831036766227788	0.32605024677428796
5	0.046243004208536866	0.22678814503925804
6	0.02603366106578644	0.15321130111007378
7	0.0168832841160867	0.11592024033360065
8	0.008665806625637084	0.06799911877816395
9	0.006446900640705456	0.05691120619534806
>10	0.02626383019557099	0.37157992205415546
>50	2.260191984893623E-5	0.0012636432739698065
>100	1.0006920705498355E-5	0.0024440163643176653
>500	8.893392830447476E-6	0.0060800137017782445
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.7400576585505736E-5	0.0	0.0	0.0
2	1.4790490097679875E-4	8.700288292752868E-6	1.7400576585505736E-5	0.0
3	0.0026100864878258606	2.6100864878258604E-5	2.6100864878258604E-5	0.0
4	0.0026796887941678837	2.6100864878258604E-5	3.480115317101147E-5	0.0
5	0.0027318905239244007	2.6100864878258604E-5	4.3501441463764344E-5	0.0
6	0.0027666916770954122	2.6100864878258604E-5	7.830259463477582E-5	0.0
7	0.0028536945600229407	3.480115317101147E-5	7.830259463477582E-5	0.0
8	0.002905896289779458	3.480115317101147E-5	9.570317122028155E-5	0.0
9	0.002984198884414234	3.480115317101147E-5	1.0440345951303442E-4	0.0
10	0.0030625014790490097	3.480115317101147E-5	1.131037478057873E-4	0.0
11	0.00312340349709828	4.3501441463764344E-5	1.131037478057873E-4	0.0
12	0.0032191066683185613	6.090201804927008E-5	1.3050432439129303E-4	0.0
13	0.003227806956611314	7.830259463477582E-5	1.3050432439129303E-4	0.0
14	0.003323510127831596	7.830259463477582E-5	1.5660518926955165E-4	0.0
15	0.003375711857588113	7.830259463477582E-5	1.653054775623045E-4	0.0
16	0.0034279135873446303	7.830259463477582E-5	1.653054775623045E-4	0.0
17	0.0035149164702721588	7.830259463477582E-5	1.653054775623045E-4	0.0
18	0.0039499308849098025	7.830259463477582E-5	1.7400576585505738E-4	0.0
19	0.00418483866881413	7.830259463477582E-5	1.7400576585505738E-4	0.0
20	0.01632174083720438	7.830259463477582E-5	1.8270605414781024E-4	0.0
21	0.017009063612331857	7.830259463477582E-5	1.914063424405631E-4	0.0
22	0.017696386387459333	7.830259463477582E-5	2.0880691902606883E-4	0.0
23	0.018609916658198385	9.570317122028155E-5	2.262074956115746E-4	0.0
24	0.019819256730891036	9.570317122028155E-5	2.3490778390432746E-4	0.0
25	0.02135050747041554	9.570317122028155E-5	2.4360807219708032E-4	0.0
26	0.023499478678725497	1.131037478057873E-4	2.523083604898332E-4	0.0
27	0.026483677563139733	1.131037478057873E-4	2.784092253680918E-4	0.0
28	0.031495043619765385	1.3050432439129303E-4	3.045100902463504E-4	0.0
29	0.03567118200028676	1.392046126840459E-4	3.045100902463504E-4	0.0
30	0.040795651804718204	1.392046126840459E-4	3.132103785391033E-4	0.0
31	0.04873031472770882	1.392046126840459E-4	3.132103785391033E-4	0.0
32	0.05927506413852529	1.4790490097679875E-4	3.132103785391033E-4	0.0
33	0.07298671848790382	1.7400576585505738E-4	3.2191066683185613E-4	0.0
34	0.09134432678561237	1.7400576585505738E-4	3.30610955124609E-4	0.0
35	0.11906344528632301	2.0010663073331597E-4	3.3931124341736186E-4	0.0
36	0.15649208552174584	2.0010663073331597E-4	3.567118200028676E-4	0.0
37	0.2109732908109643	2.0880691902606883E-4	3.654121082956205E-4	0.0
38	0.2852215511013173	2.1750720731882173E-4	3.828126848811262E-4	0.0
39	0.37379918620983427	2.1750720731882173E-4	4.0021326146663194E-4	0.0
40	0.480725729327767	2.1750720731882173E-4	4.2631412634489056E-4	0.0
41	0.59454290077356	2.1750720731882173E-4	4.3501441463764345E-4	0.0
42	0.7489991188348017	2.262074956115746E-4	4.61115279515902E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAGCGGT	4985	0.0	10.942134	48
CCGAGAC	990	0.0	10.922415	48
ACGAGCG	255	2.6376298E-5	10.3617325	47
CGCTCGG	305	1.6506365E-6	10.229071	41
AGCGGTT	3960	0.0	9.8301735	48
GCGGTTC	2930	0.0	9.759345	48
ATGCCGA	1110	0.0	9.68792	14
GCGGGTC	1215	0.0	9.493062	48
GGTTCTT	3825	0.0	9.33455	1
AAGAGCG	8890	0.0	9.2441225	48
AGAGCGG	7895	0.0	9.222142	48
CGCGGTT	265	4.3986173E-4	9.067664	48
GCGATCG	400	5.804868E-7	9.000166	42
GACCGGT	250	0.0026854784	8.625273	34
CGGAAGG	1630	0.0	8.550308	48
GGTTTTA	5210	0.0	8.508883	1
GGTCTTT	4570	0.0	8.389622	1
AATGCCG	1265	0.0	8.311919	13
CGATCGG	1130	0.0	8.284206	44
GGTTATT	3570	0.0	8.256109	1
>>END_MODULE
