FastQCFastQC Report
Sun 14 Aug 2016
V9M_input.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameV9M_input.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11493872
Sequences flagged as poor quality0
Sequence length54
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGT49850.010.94213448
CCGAGAC9900.010.92241548
ACGAGCG2552.6376298E-510.361732547
CGCTCGG3051.6506365E-610.22907141
AGCGGTT39600.09.830173548
GCGGTTC29300.09.75934548
ATGCCGA11100.09.6879214
GCGGGTC12150.09.49306248
GGTTCTT38250.09.334551
AAGAGCG88900.09.244122548
AGAGCGG78950.09.22214248
CGCGGTT2654.3986173E-49.06766448
GCGATCG4005.804868E-79.00016642
GACCGGT2500.00268547848.62527334
CGGAAGG16300.08.55030848
GGTTTTA52100.08.5088831
GGTCTTT45700.08.3896221
AATGCCG12650.08.31191913
CGATCGG11300.08.28420644
GGTTATT35700.08.2561091
Produced by FastQC (version 0.11.3)