##FastQC	0.11.3
>>Basic Statistics	pass
#Measure	Value
Filename	V9M_H3K4me3_ChIPSeq.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	34673675
Sequences flagged as poor quality	0
Sequence length	36
%GC	63
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	34.745525070532615	39.0	36.0	40.0	23.0	40.0
2	34.29551266198348	39.0	36.0	40.0	14.0	40.0
3	34.28027643449966	39.0	36.0	40.0	14.0	40.0
4	34.26140750872239	39.0	36.0	40.0	12.0	40.0
5	34.01499422833028	39.0	35.0	40.0	2.0	40.0
6	34.00042579853448	39.0	36.0	40.0	2.0	40.0
7	33.93858314701283	39.0	35.0	40.0	2.0	40.0
8	33.790209142815115	39.0	35.0	40.0	2.0	40.0
9	33.854946209191844	39.0	35.0	40.0	2.0	40.0
10	33.78701478859683	39.0	35.0	40.0	2.0	40.0
11	33.95083910199885	39.0	36.0	40.0	2.0	40.0
12	33.89512937408567	39.0	36.0	40.0	2.0	40.0
13	33.89385829451306	39.0	36.0	40.0	2.0	40.0
14	33.85759764432238	39.0	35.0	40.0	2.0	40.0
15	33.57923320790196	39.0	35.0	40.0	2.0	40.0
16	33.54911240299737	39.0	35.0	40.0	2.0	40.0
17	33.65464851937385	39.0	35.0	40.0	2.0	40.0
18	33.58485176434284	39.0	35.0	40.0	2.0	40.0
19	33.534138016809585	39.0	35.0	40.0	2.0	40.0
20	33.43194160988127	39.0	35.0	40.0	2.0	40.0
21	33.375632435846505	39.0	35.0	40.0	2.0	40.0
22	33.2223620080652	39.0	35.0	40.0	2.0	40.0
23	33.16791842802933	39.0	35.0	40.0	2.0	40.0
24	33.07760809893961	39.0	35.0	40.0	2.0	40.0
25	32.9378766167705	39.0	34.0	40.0	2.0	40.0
26	32.58762565548648	39.0	33.0	40.0	2.0	40.0
27	32.412710968768096	38.0	33.0	40.0	2.0	40.0
28	32.18089824629204	38.0	33.0	40.0	2.0	40.0
29	32.02581335840519	38.0	33.0	40.0	2.0	40.0
30	31.811286170271828	38.0	32.0	40.0	2.0	40.0
31	31.653731397090155	38.0	32.0	40.0	2.0	40.0
32	31.668691536158196	38.0	32.0	40.0	2.0	40.0
33	31.663828971114253	38.0	32.0	40.0	2.0	40.0
34	31.576669043589987	38.0	32.0	40.0	2.0	40.0
35	31.437342508401546	38.0	32.0	40.0	2.0	40.0
36	31.24295800776814	38.0	32.0	40.0	2.0	40.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1	1	-0.26866184491115774
1	2	-0.2750204748711269
1	3	-0.24011383708035794
1	4	-0.22554897377311534
1	5	0.07178095412724872
1	6	0.02014137492104595
1	7	-8.766082141775655E-4
1	8	-0.06971028181523309
1	9	-0.0021443214934180332
1	10	0.035274082891866954
1	11	-0.22254335046243057
1	12	-0.05086474729767332
1	13	-0.05020852508358331
1	14	-0.07518517360626475
1	15	0.12518357339942554
1	16	0.0726880602994413
1	17	0.010206788705211522
1	18	0.009805730399399692
1	19	-4.326165354910927E-4
1	20	0.015024498477842485
1	21	0.03913684988842192
1	22	0.12844748156961572
1	23	0.07538341458320019
1	24	0.09802520618774224
1	25	0.13004277150664478
1	26	0.2259024827831695
1	27	0.19316306010208706
1	28	0.2981997865818258
1	29	0.2922404520910291
1	30	0.3622823707573595
1	31	0.16764397826939614
1	32	0.4673819286153922
1	33	0.23504206353255697
1	34	0.3592786467647926
1	35	0.3934072560149495
1	36	0.49667165907061417
2	1	-0.47463763472754295
2	2	-0.46789468251727584
2	3	-0.3357408787555869
2	4	-0.4783701415955264
2	5	-0.1685682882224171
2	6	-0.10596028715020367
2	7	-0.09963794864755471
2	8	-0.1942170341114675
2	9	-0.11288372667324609
2	10	-0.07000803445699688
2	11	-0.2350322598345116
2	12	-0.17158506697082743
2	13	-0.19108744567435565
2	14	-0.2276140220487335
2	15	0.01863941507163247
2	16	0.027358458918364192
2	17	-0.028864827532196102
2	18	-0.10877388819222489
2	19	-0.05156639737312929
2	20	-0.06111313135979657
2	21	-0.03164012205503042
2	22	0.04907575778919693
2	23	-0.005253904241477869
2	24	-0.007931994049869218
2	25	0.06399536174443199
2	26	0.16802378152062403
2	27	0.02905956718114311
2	28	0.17586403773062642
2	29	0.43348854608916554
2	30	0.3161305014856559
2	31	0.39959810311312793
2	32	0.2388742829142778
2	33	0.3379401718870483
2	34	0.3233305283657195
2	35	0.3254336688671913
2	36	0.3999717283063049
3	1	-0.40664138058006216
3	2	-0.30937073173540597
3	3	-0.3068173995113668
3	4	-0.4018700602010483
3	5	0.014381361670920967
3	6	-0.03471690240519365
3	7	0.0013144021660096428
3	8	0.03063778309788745
3	9	0.0015354100855446973
3	10	0.01951579822205929
3	11	-0.09904731913256626
3	12	-0.11628520154833666
3	13	-0.17037687982436722
3	14	-0.14716245016810348
3	15	0.08668989091904677
3	16	0.11130394598551163
3	17	-0.007190565325494447
3	18	-0.07006706294291831
3	19	0.006019287414758878
3	20	-0.0017389707673274302
3	21	-0.014866924216960342
3	22	0.10650747669151883
3	23	0.042379055023523904
3	24	0.008706299081019608
3	25	0.047318213972999956
3	26	0.05341118014309387
3	27	-0.08542958145628177
3	28	-0.016733295413125404
3	29	0.22971418722160308
3	30	0.228705313861024
3	31	0.3357498223877151
3	32	0.24080021205104885
3	33	0.2233542706265652
3	34	0.2357315586023958
3	35	0.180281382448225
3	36	0.43464409303789964
4	1	-0.6191171261713038
4	2	-0.5962380244609804
4	3	-0.5099018778514619
4	4	-0.5264432537859562
4	5	-0.18782726275665595
4	6	-0.18129526512077376
4	7	-0.14188819981352907
4	8	-0.10110467009715762
4	9	-0.10777878171836619
4	10	-0.10667834621110472
4	11	-0.16944981885822585
4	12	-0.2236266428798075
4	13	-0.1999475644453952
4	14	-0.19594909810722783
4	15	-0.024995311172048673
4	16	0.020029812507871725
4	17	-0.12361151809736981
4	18	-0.08782603332961259
4	19	-0.0741269408533185
4	20	-0.0723354166771486
4	21	-0.059291014669987874
4	22	0.015283946795676684
4	23	-0.01133981089159164
4	24	-0.00875039905776731
4	25	0.025500514860162582
4	26	0.14647887089291345
4	27	0.12414637720949884
4	28	0.00788416828413574
4	29	0.18108937169344586
4	30	0.3144446668786749
4	31	0.3659288531008684
4	32	0.4250424912545476
4	33	0.2797909500693123
4	34	0.40810519628590214
4	35	0.3302364744848134
4	36	0.35796034275800537
5	1	-0.5996210534897699
5	2	-0.5194329902190802
5	3	-0.4549496732279721
5	4	-0.4708230738154384
5	5	-0.13037498895053545
5	6	-0.16765297789273603
5	7	-0.10143279548074702
5	8	-0.04484456010909099
5	9	-0.09825761562654378
5	10	-0.07117568946677721
5	11	-0.18263760999308687
5	12	-0.17247389655207712
5	13	-0.15749083600860558
5	14	-0.1807781532796966
5	15	0.038320822691595424
5	16	0.10723621413765017
5	17	-0.05292115840509126
5	18	-0.057827480961911704
5	19	-0.032222736213114445
5	20	-0.03225535857534112
5	21	-0.004286158199093393
5	22	0.07237162329096947
5	23	0.005793865483077809
5	24	0.033997539621552164
5	25	-0.010831284806030794
5	26	0.17261951520289642
5	27	-0.08883376529841769
5	28	0.31363199419010357
5	29	0.28062120946010083
5	30	0.2711060874796054
5	31	0.3384405930299188
5	32	0.15922023782656325
5	33	0.33573674144271237
5	34	0.3716600181899743
5	35	0.3128569747615124
5	36	0.35295782221048455
6	1	-0.3023415524521482
6	2	-0.29731854629587673
6	3	-0.2525419446930215
6	4	-0.24917117679538592
6	5	0.05782145873546796
6	6	0.05897619177700619
6	7	0.029116186632656138
6	8	0.11588202531936531
6	9	0.08685156134242789
6	10	0.1035397411485377
6	11	-0.013777434228437357
6	12	-0.010798343295576274
6	13	0.013032022956927847
6	14	-0.031166099202231123
6	15	0.18312295296787084
6	16	0.17274562627670775
6	17	0.035947579102824534
6	18	0.06842640831050772
6	19	0.06277110392501584
6	20	0.06386583421963365
6	21	0.07274097648890177
6	22	0.1558516708102644
6	23	0.11662903222897114
6	24	0.11046447916661606
6	25	0.07850127754058889
6	26	0.09287567367199046
6	27	-0.2072942824513575
6	28	0.05186985761233842
6	29	-0.10035473112541027
6	30	0.08556499864698353
6	31	0.26955383600331473
6	32	0.14032886859595806
6	33	0.20650835648368826
6	34	0.2994752454156284
6	35	0.17961345085374703
6	36	0.4411112841106899
7	1	-0.3536758529221231
7	2	-0.30569649345834193
7	3	-0.4447853948169893
7	4	-0.3393673158555828
7	5	0.018635581535178858
7	6	-0.016253395765012613
7	7	-0.02848366808345304
7	8	-0.11023977185219991
7	9	-0.00508825078325259
7	10	-0.016136618943384917
7	11	-0.17100595741428748
7	12	-0.12439202173024455
7	13	-0.15187345176615707
7	14	-0.11546830007164743
7	15	0.03155451321688929
7	16	0.05379586702967032
7	17	-0.04572845419695426
7	18	-0.030349504233448954
7	19	-0.04232452759603689
7	20	-0.010169892376694634
7	21	0.00821893824876696
7	22	0.06420022567524342
7	23	0.005930542279223516
7	24	0.07606530650198096
7	25	0.10524968577813354
7	26	0.14438798881146653
7	27	0.2917481071986998
7	28	0.10673487390754843
7	29	0.21327654334546509
7	30	0.21911959466681807
7	31	0.30813738928870293
7	32	0.3115425253745556
7	33	0.30406573021841155
7	34	0.23800744436863752
7	35	0.17280501622005673
7	36	0.3099003840051644
8	1	-0.40839839259698607
8	2	-0.293427548472458
8	3	-0.28617491025036657
8	4	-0.32584048191364445
8	5	-0.052854131804295434
8	6	-0.03849588256045422
8	7	-0.03748552106591063
8	8	-0.12200289752512106
8	9	-0.022178357121333647
8	10	-0.0405511363479647
8	11	-0.1671646912488356
8	12	-0.11865861581686232
8	13	-0.12413806883663625
8	14	-0.11643110705940529
8	15	0.05639734633918181
8	16	0.08670854103991132
8	17	-0.03605286873735736
8	18	-0.05797264255906498
8	19	-0.046854666017466684
8	20	-0.00433317708011316
8	21	0.02726705079056302
8	22	0.08843243131665446
8	23	0.006012299959337497
8	24	0.07825335768262676
8	25	0.1026890592995926
8	26	0.11026435012581004
8	27	0.11310523763089009
8	28	-0.01606315777173961
8	29	0.14602102003837558
8	30	-0.07335150090021969
8	31	0.2717839059130327
8	32	0.2391314289933284
8	33	0.337941979086942
8	34	0.27656309213891817
8	35	0.17862559596986216
8	36	0.45153064779152174
9	1	-0.28718535730432393
9	2	-0.3580283295524964
9	3	-0.4500203015366182
9	4	-0.3466186922975609
9	5	-0.05441986607578997
9	6	-0.01775357859091997
9	7	-0.07290715280677063
9	8	-0.11388796820029512
9	9	-0.07444328005831125
9	10	0.020129936180616426
9	11	-0.11027237300552173
9	12	-0.1787867144568125
9	13	-0.082254179624357
9	14	-0.14005042528606992
9	15	0.0723397094758127
9	16	0.09418605985392503
9	17	-0.029108096769853375
9	18	-0.0481601979415629
9	19	-0.01582197647262973
9	20	0.03464491459129704
9	21	-0.01966954174520197
9	22	0.05344022481212107
9	23	-0.006921549347481459
9	24	0.011945242688945257
9	25	-0.03855167392029557
9	26	0.12374720406948114
9	27	0.1047737512689011
9	28	0.017546160796349852
9	29	0.03740050530373651
9	30	0.24403651236258028
9	31	0.2332843656943524
9	32	0.11675139746538576
9	33	0.1673637020667833
9	34	0.18533776118868772
9	35	-0.010597469311594665
9	36	0.11775421068635694
10	1	-0.3807061792015247
10	2	-0.49485470788957286
10	3	-0.5037811511196963
10	4	-0.4279682658257329
10	5	-0.19067519742687722
10	6	-0.13013807698616375
10	7	-0.1091966768342374
10	8	-0.19419350887005038
10	9	-0.047965451051034336
10	10	-0.06183528479233047
10	11	-0.234463413818915
10	12	-0.08571158410327939
10	13	-0.20719082194498384
10	14	-0.1582453098592893
10	15	-0.010304067272336681
10	16	0.06102047542491107
10	17	-0.06001756602044139
10	18	-0.08613912686791991
10	19	-0.053067614878905545
10	20	-0.08974223592741026
10	21	-0.06121536789382276
10	22	-0.08797025779976764
10	23	-0.08455481380338625
10	24	-0.03492545479491582
10	25	-0.12044075939947163
10	26	-0.26164268799796275
10	27	-0.04220382902001063
10	28	-0.5549403174630534
10	29	-0.0749529631012571
10	30	0.11465989029963097
10	31	0.2604022189027013
10	32	0.04254166117938496
10	33	0.09819231188140876
10	34	0.020021714404101942
10	35	0.06636373712440857
10	36	0.03840723761891596
11	1	-0.5293882336299234
11	2	-0.46353376520053047
11	3	-0.3738969193068371
11	4	-0.4380499238739475
11	5	-0.1282340126466721
11	6	-0.14855529966732917
11	7	-0.14916883018973692
11	8	-0.41468285723568954
11	9	-0.1572219702277664
11	10	-0.137345153869731
11	11	-0.2833310952069823
11	12	-0.21626772387233473
11	13	-0.22961463213986377
11	14	-0.23515937177896973
11	15	-0.03913047776841694
11	16	-0.006190515186162315
11	17	-0.1302351642466988
11	18	-0.09203495582161736
11	19	-0.10365848591421667
11	20	-0.09358121131735686
11	21	-0.05387318248636319
11	22	-0.062469468535475414
11	23	-0.046974622679101685
11	24	0.007200774931511944
11	25	-0.0121949764233662
11	26	0.060268652436491266
11	27	-0.07691835822804194
11	28	-0.07052692263216898
11	29	-0.12244018759160014
11	30	-0.08375971587384967
11	31	0.1742480763401133
11	32	0.01836554671629287
11	33	0.15683421777390194
11	34	0.21736483192283984
11	35	0.09962677917406637
11	36	0.2905131457307739
12	1	-0.4512733483612408
12	2	-0.36801652547215724
12	3	-0.3954590033917711
12	4	-0.33507697798545877
12	5	-0.06088011412500549
12	6	-0.10136281093635091
12	7	-0.0714918372628972
12	8	-0.012111757060772277
12	9	-0.060981114742915565
12	10	-0.06312925457046248
12	11	-0.33210519253015036
12	12	-0.11872345633638304
12	13	-0.11375814333779743
12	14	-0.11524372496934632
12	15	0.07450432674493612
12	16	0.08653726604858036
12	17	-0.026283939156058977
12	18	-0.06843280600714508
12	19	-0.03485556602961992
12	20	0.0032868667348253666
12	21	0.03499857868214917
12	22	0.08363678378938744
12	23	0.022665836121348093
12	24	0.08898539753771217
12	25	-0.08084831553549776
12	26	0.1472117905878747
12	27	0.025458362760495845
12	28	0.053102355605851415
12	29	-0.05402282356822852
12	30	0.24994311007709413
12	31	0.4727147814483246
12	32	0.224771127934428
12	33	0.3450409274997348
12	34	0.3463190374040934
12	35	0.2741259476287716
12	36	0.34239108156916487
13	1	-0.4391468419501905
13	2	-0.4749434109543813
13	3	-0.45239426777730785
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115	5	0.2558406819127512
115	6	0.27501785289551606
115	7	0.2587895840665837
115	8	0.31660475064101234
115	9	0.2761385773493501
115	10	0.29957894665687235
115	11	0.18297497770076632
115	12	0.20500541520176085
115	13	0.1529050976143651
115	14	0.1528790664472055
115	15	0.34357764948097724
115	16	0.36035035086194256
115	17	0.19730785974464737
115	18	0.1285211234093211
115	19	0.16401524115267563
115	20	0.22355284332913783
115	21	0.20183328365713749
115	22	0.2233461809782895
115	23	0.1766823342440773
115	24	0.20700754912772368
115	25	0.23627606098658305
115	26	0.14910134247862317
115	27	0.03477785855696425
115	28	0.42234602562563595
115	29	0.547874135949229
115	30	0.5562158057588711
115	31	0.47701068369621424
115	32	0.3191435651162351
115	33	0.32225197195116806
115	34	0.25322377513643346
115	35	0.199147534728791
115	36	0.19926620544544704
116	1	-0.13521865438708858
116	2	-0.048675507299385856
116	3	-0.05449342198846807
116	4	-0.026196835284203246
116	5	0.21428535498140633
116	6	0.16726125090246313
116	7	0.16046035666470715
116	8	0.20461983631421532
116	9	0.18199681864646067
116	10	0.17119402080088264
116	11	0.006900974707384933
116	12	0.05169872616424698
116	13	-0.015875295159844427
116	14	-0.005914802596173274
116	15	0.1557444892060147
116	16	0.22705848504942594
116	17	0.04780721778558217
116	18	0.09157684261328569
116	19	0.06673187403663405
116	20	0.062310865547125616
116	21	0.08353460829017934
116	22	0.11989416284131948
116	23	0.12283374334632668
116	24	0.037581001210597265
116	25	0.16018530058136804
116	26	0.13244183014896294
116	27	0.09425155648885664
116	28	0.21452862334463418
116	29	0.24184099092256872
116	30	0.44949260633299915
116	31	0.48961312331618245
116	32	0.36254550580457234
116	33	0.28759064210876417
116	34	0.2574482359388206
116	35	0.22031723027881966
116	36	0.26570326103519903
117	1	-0.14967123599299725
117	2	-0.09178020739913251
117	3	-0.09108660897029353
117	4	-0.043242997227523006
117	5	0.1896330324272668
117	6	0.16247430247229033
117	7	0.1400978954181582
117	8	0.22212322220477176
117	9	0.14835968510227815
117	10	0.2100778910892842
117	11	0.0843722660288293
117	12	0.06458312424435064
117	13	0.048959304709732976
117	14	0.03705164579569953
117	15	0.19573593595663397
117	16	0.2464581302564497
117	17	0.08653620975482568
117	18	0.08744507073499364
117	19	0.08340921732506246
117	20	0.1302870142781103
117	21	0.11051033366278062
117	22	0.18918443996653878
117	23	0.1716015993510851
117	24	0.16236348793739808
117	25	0.2106836520478481
117	26	0.22897009788027844
117	27	0.24689654314148157
117	28	0.3111626817726858
117	29	0.38311941336437627
117	30	0.44667582976021336
117	31	0.46573928708841805
117	32	0.4255367790559639
117	33	0.3924449803096657
117	34	0.2970823432372711
117	35	0.34462577208940104
117	36	0.4301404505920736
118	1	0.11593237146180968
118	2	0.1971408909517791
118	3	0.13796279943013445
118	4	0.21610137148826425
118	5	0.41465608723329694
118	6	0.4653545005882975
118	7	0.41994332849134963
118	8	0.466242516634793
118	9	0.4442925941532465
118	10	0.43254560386331775
118	11	0.2512347864703699
118	12	0.3317910297916171
118	13	0.31152672015958416
118	14	0.3178447605162731
118	15	0.48328641706307707
118	16	0.5257673749885257
118	17	0.3550421535545709
118	18	0.3707961323119946
118	19	0.36009486765580334
118	20	0.40320300097342
118	21	0.3254471593734607
118	22	0.3838777897425203
118	23	0.38118312215133443
118	24	0.31183739326522897
118	25	0.4284031928672931
118	26	0.32128116806013907
118	27	0.3967349417272814
118	28	0.5860134890381374
118	29	0.5686772002798435
118	30	0.534181521677251
118	31	0.664322721399607
118	32	0.6801401635270956
118	33	0.6126355382737181
118	34	0.5404217289434676
118	35	0.6117651494502816
118	36	0.6805923335999999
119	1	-0.04356259956332309
119	2	0.007923691171278335
119	3	0.07271743329322078
119	4	0.06048548620394456
119	5	0.2822384687760149
119	6	0.25990398797975445
119	7	0.28571667902174624
119	8	0.18824335709656737
119	9	0.2807584846026714
119	10	0.26259968433306824
119	11	0.1546359421655623
119	12	0.22000197335518124
119	13	0.16905809557074036
119	14	0.11205694956390744
119	15	0.3010384803830135
119	16	0.3340079466371577
119	17	0.15840798043540616
119	18	0.18635928636980736
119	19	0.13985635307329147
119	20	0.193856371908268
119	21	0.14231677419132183
119	22	0.1585011370857643
119	23	0.20736793598027958
119	24	0.19777311152761712
119	25	0.2907661410024147
119	26	0.3646809366983703
119	27	0.48376413603501334
119	28	0.5845748807690327
119	29	0.6090218575467148
119	30	0.6963736982937299
119	31	0.7116904099618608
119	32	0.5988864605878241
119	33	0.46956719958150117
119	34	0.41606957242726494
119	35	0.41029365052040845
119	36	0.4768324950737082
120	1	-2.2225529112729916
120	2	-2.217284724362692
120	3	-2.2313645028715
120	4	-2.2348968495140866
120	5	-2.0307631706226985
120	6	-2.2053735629383127
120	7	-2.272740917077538
120	8	-2.3666924996576917
120	9	-2.3560114622057817
120	10	-2.413306245845039
120	11	-5.929271930000986
120	12	-6.794156378668305
120	13	-5.506176703689576
120	14	-5.186974998724462
120	15	-23.392879971716535
120	16	-26.53462820476297
120	17	-8.452445540665117
120	18	-8.268144447888123
120	19	-8.449229344906076
120	20	-8.161942286380011
120	21	-4.69464176391941
120	22	-4.941665039034586
120	23	-4.6852654608792506
120	24	-4.789649073374804
120	25	-4.654438244449203
120	26	-2.557047783969608
120	27	-2.469999961996052
120	28	-2.244336631683197
120	29	-2.226625044608575
120	30	-2.058286754675091
120	31	-2.0328085515528045
120	32	-2.077572913672668
120	33	-2.1105158876568773
120	34	-2.0259738663090516
120	35	-1.9725613316928303
120	36	-2.0459375528928696
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	3202640.0
3	66190.0
4	100957.0
5	212076.0
6	64617.0
7	55639.0
8	62470.0
9	64113.0
10	65341.0
11	54484.0
12	58431.0
13	64979.0
14	68993.0
15	68344.0
16	72337.0
17	76432.0
18	81858.0
19	87099.0
20	95702.0
21	111577.0
22	131978.0
23	149765.0
24	164415.0
25	183920.0
26	206812.0
27	232415.0
28	261537.0
29	293846.0
30	338339.0
31	409010.0
32	498541.0
33	625358.0
34	849181.0
35	1219921.0
36	1841605.0
37	3068804.0
38	4781306.0
39	1.4491532E7
40	191111.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.84331620986746	19.806852500695484	12.189253712634256	34.160577576802794
2	34.066249420698185	20.579689208273575	18.151831978323102	27.202229392705142
3	31.22062530127756	19.394392310617555	18.496554233688954	30.88842815441593
4	33.256846828674355	19.230787043948304	17.43702971176652	30.07533641561082
5	33.569839489420275	20.144628346343566	18.085222164235574	28.200310000000584
6	33.18420527141997	18.71218536694861	16.36676349739169	31.736845864239726
7	32.18788602721012	19.386564424489983	18.142194101802925	30.283355446496973
8	32.08962004174585	18.276072194970304	18.281803242821315	31.352504520462528
9	32.20871951912403	17.695755039961305	18.03230120597773	32.06322423493693
10	32.29729552073601	19.445029464458088	16.99234313822342	31.265331876582486
11	33.56430645488422	18.193989974316462	17.22498967934036	31.01671389145896
12	32.97739702212852	17.957498246884555	17.536727210862512	31.528377520124412
13	32.53847657021985	18.570593419646897	17.741143955732223	31.14978605440103
14	32.308479497515926	18.62937995172553	17.728851700623878	31.33328885013468
15	31.910763443882008	18.07760178411706	18.613528013387707	31.398106758613224
16	32.58779154202121	18.009975721485	17.986907923104617	31.415324813389184
17	31.934047591640596	18.151190893406355	17.624072777203267	32.29068873774978
18	32.05388887111679	18.51668838637683	17.559575718105698	31.869847024400677
19	33.13770614838683	18.008740816415326	17.51458017823646	31.338972856961384
20	32.223734696045156	18.55517052863682	17.489782490153154	31.731312285164865
21	32.603319231831016	18.45787476328427	17.46749061171714	31.471315393167576
22	32.508904710862446	18.50663512088515	17.528980629764682	31.455479538487722
23	32.31741812804829	18.0932716183946	18.066916993290675	31.52239326026644
24	32.0652607161212	18.469010910239835	17.604257961225724	31.861470412413233
25	32.620417574523245	18.518781542121705	17.517086274019007	31.343714609336047
26	32.726876699902064	18.197486329661697	18.02117430762751	31.054462662808728
27	32.860092492352734	18.526602043887934	17.573555762969807	31.039749700789525
28	32.5817388959153	18.532610733478748	17.443417226016123	31.442233144589828
29	33.055450823078786	18.11106631756251	17.522197061501128	31.311285797857575
30	33.04216708634765	18.53252666015804	17.385330656464966	31.03997559702934
31	33.166854161629175	18.470814375150542	17.34969637820102	31.012635085019262
32	32.91924503111518	18.087634012990552	17.507698674396885	31.485422281497378
33	32.77101927834011	18.60496988713532	17.435836954436677	31.188173880087888
34	32.80293447056061	18.08829107673196	17.7246986256432	31.38407582706423
35	32.33312989685916	18.247106856586026	17.879100470914167	31.540662775640644
36	32.75331344356335	18.343881833185325	17.824770139848706	31.07803458340262
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	94647.0
1	84386.5
2	74126.0
3	74126.0
4	50523.5
5	26921.0
6	26921.0
7	25586.5
8	24252.0
9	24252.0
10	25295.0
11	26338.0
12	26338.0
13	27597.5
14	28857.0
15	30974.5
16	33092.0
17	33092.0
18	36266.0
19	39440.0
20	39440.0
21	46211.5
22	52983.0
23	52983.0
24	65364.0
25	77745.0
26	97293.5
27	116842.0
28	116842.0
29	146814.0
30	176786.0
31	176786.0
32	215766.5
33	254747.0
34	254747.0
35	306988.0
36	359229.0
37	359229.0
38	423754.5
39	488280.0
40	569331.0
41	650382.0
42	650382.0
43	752673.0
44	854964.0
45	854964.0
46	985785.5
47	1116607.0
48	1116607.0
49	1279938.0
50	1443269.0
51	1624369.5
52	1805470.0
53	1805470.0
54	2011211.0
55	2216952.0
56	2216952.0
57	2506575.5
58	2796199.0
59	2796199.0
60	2827044.0
61	2857889.0
62	2857889.0
63	2864919.5
64	2871950.0
65	2866400.5
66	2860851.0
67	2860851.0
68	2805753.5
69	2750656.0
70	2750656.0
71	2651451.5
72	2552247.0
73	2552247.0
74	2413495.5
75	2274744.0
76	2096696.5
77	1918649.0
78	1918649.0
79	1711533.0
80	1504417.0
81	1504417.0
82	1284682.0
83	1064947.0
84	1064947.0
85	865293.5
86	665640.0
87	665640.0
88	511614.0
89	357588.0
90	258712.0
91	159836.0
92	159836.0
93	108695.5
94	57555.0
95	57555.0
96	36770.0
97	15985.0
98	15985.0
99	9289.0
100	2593.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.9746500767513107
2	1.0592820057291303
3	0.9766544792266756
4	0.9892230921585323
5	1.157699609285719
6	1.0665872596429422
7	1.0318058296387678
8	1.216049351561379
9	1.0179913147366122
10	1.0616296080527952
11	1.2310001752049646
12	1.1013138930326827
13	1.0579293945623012
14	1.0630197116400266
15	1.182464794977746
16	1.1433890408213148
17	1.1091584609938232
18	1.10018335235593
19	1.1034278887369164
20	1.1074943743344194
21	1.1093632272898677
22	1.1551241684073004
23	1.1722005238844744
24	1.0891173202725122
25	1.0720698051187247
26	1.1578813033230542
27	1.0479967871879747
28	1.009474767240565
29	1.0116493276239107
30	1.009368058044035
31	1.014787154808367
32	1.0119579190841468
33	1.0117271964970542
34	1.007262714436817
35	1.0002545158538863
36	1.0011947103962877
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
36	3.4673675E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	71.48830942786844
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.4203333551785	56.77625365759461
2	12.665385731574789	18.108540284042565
3	4.469272103420599	9.585021211400168
4	1.7833014712766018	5.0994082952717905
5	0.7449548227201597	2.6627780438200825
6	0.3520910539955175	1.5102236528889532
7	0.1786827982348595	0.8941611818755715
8	0.09992364338534598	0.5714697869993275
9	0.06760260453868862	0.4349516320252437
>10	0.20883659418451506	2.2473904475877147
>50	0.0055625414439416316	0.2830053749241552
>100	0.003894057511057797	0.49080904037631967
>500	1.0668238559981344E-4	0.04706096626017656
>1k	1.22480030926785E-5	0.023907566873170517
>5k	4.029214712794022E-6	0.01821576015234445
>10k+	3.626293209516469E-5	1.2468030979076916
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTT	134956	0.389217468295472	Illumina Paired End PCR Primer 2 (96% over 25bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCG	68080	0.19634492161560607	Illumina Paired End PCR Primer 2 (97% over 36bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCG	64148	0.18500490646001616	Illumina Paired End PCR Primer 2 (100% over 36bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	60560	0.17465699842892338	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	9.113542190148579E-4	2.8840323386546134E-6	0.0	1.730419403192768E-5
2	0.0010786280946568254	2.8840323386546134E-6	0.0	2.3072258709236907E-5
3	0.001542957301180218	5.768064677309227E-6	0.0	2.8840323386546134E-5
4	0.0019121134405280086	5.768064677309227E-6	0.0	4.32604850798192E-5
5	0.0024254711968085298	5.768064677309227E-6	0.0	4.6144517418473814E-5
6	0.002780207174463047	5.768064677309227E-6	0.0	6.056467911174688E-5
7	0.003178203637197384	8.65209701596384E-6	0.0	7.210080846636534E-5
8	0.0034694909034015	8.65209701596384E-6	0.0	8.363693782098379E-5
9	0.003962660433311439	8.65209701596384E-6	0.0	1.0094113185291146E-4
10	0.004450061898544068	8.65209701596384E-6	0.0	1.095932288688753E-4
11	0.0051105053040959745	8.65209701596384E-6	0.0	1.2401339056214837E-4
12	0.0060016712967402505	8.65209701596384E-6	0.0	1.2401339056214837E-4
13	0.006797664222208923	1.1536129354618453E-5	0.0	1.2689742290080297E-4
14	0.007484063918808722	1.1536129354618453E-5	0.0	1.3554951991676684E-4
15	0.028003954008336295	1.1536129354618453E-5	0.0	1.3843355225542144E-4
16	0.029373869369197236	1.1536129354618453E-5	0.0	1.4420161693273067E-4
17	0.03178780443665115	1.1536129354618453E-5	0.0	1.4996968161003988E-4
18	0.03391622030257825	1.1536129354618453E-5	0.0	1.6150581096465835E-4
19	0.037255929750740296	1.1536129354618453E-5	0.0	1.6438984330331295E-4
20	0.45415434043261926	1.4420161693273067E-5	0.0	1.730419403192768E-4
21	0.46310926084414183	1.4420161693273067E-5	2.8840323386546134E-6	1.8457806967389525E-4
22	0.46612307463803593	1.4420161693273067E-5	5.768064677309227E-6	2.0188226370582293E-4
23	0.4700568947479608	1.4420161693273067E-5	8.65209701596384E-6	2.0765032838313216E-4
24	0.47542407893019706	1.4420161693273067E-5	8.65209701596384E-6	2.134183930604414E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCGTAT	3110	0.0	21.698883	28
TCGTATG	3250	0.0	20.85817	29
GCGGTTC	22975	0.0	19.289783	11
TCTCGTA	3535	0.0	19.19443	27
GACCGAT	3585	0.0	19.138456	21
ACCGATC	3920	0.0	17.579834	22
AGATCGG	25645	0.0	17.428288	20
ATCTCGT	4125	0.0	16.333406	26
CGTATGC	4275	0.0	15.828695	30
ATGCCGA	35685	0.0	14.335271	14
AATGCCG	36220	0.0	14.239394	13
CGGTTCA	35985	0.0	14.194742	1
CGAGATC	31915	0.0	14.162873	18
CCGAGAT	32180	0.0	14.06905	17
CCGATCT	4965	0.0	13.973683	23
GAATGCC	38140	0.0	13.550938	12
GTTCAGC	38330	0.0	13.309016	3
GAGATCG	34235	0.0	13.23417	19
TTCAGCA	38675	0.0	13.201687	4
GGTTCAG	39680	0.0	12.94518	2
>>END_MODULE
