FastQCFastQC Report
Sun 14 Aug 2016
V9M_H3K4me3_ChIPSeq.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameV9M_H3K4me3_ChIPSeq.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34673675
Sequences flagged as poor quality0
Sequence length36
%GC63

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTT1349560.389217468295472Illumina Paired End PCR Primer 2 (96% over 25bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCG680800.19634492161560607Illumina Paired End PCR Primer 2 (97% over 36bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCG641480.18500490646001616Illumina Paired End PCR Primer 2 (100% over 36bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN605600.17465699842892338No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT31100.021.69888328
TCGTATG32500.020.8581729
GCGGTTC229750.019.28978311
TCTCGTA35350.019.1944327
GACCGAT35850.019.13845621
ACCGATC39200.017.57983422
AGATCGG256450.017.42828820
ATCTCGT41250.016.33340626
CGTATGC42750.015.82869530
ATGCCGA356850.014.33527114
AATGCCG362200.014.23939413
CGGTTCA359850.014.1947421
CGAGATC319150.014.16287318
CCGAGAT321800.014.0690517
CCGATCT49650.013.97368323
GAATGCC381400.013.55093812
GTTCAGC383300.013.3090163
GAGATCG342350.013.2341719
TTCAGCA386750.013.2016874
GGTTCAG396800.012.945182
Produced by FastQC (version 0.11.3)