FastQCFastQC Report
Sun 14 Aug 2016
V9M_H3K4me1_ChIPSeq.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameV9M_H3K4me1_ChIPSeq.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14877116
Sequences flagged as poor quality0
Sequence length142
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGAC17150.028.17318772-73
CGGTTCG9700.028.06628470-71
ATCGGAG57650.027.68472570-71
ATCGGAT35400.025.37857670-71
GATCGAA24800.024.97074372-73
AGCGGTA34650.024.55293570-71
AGCGGTC39750.024.48474370-71
GATCGAT17800.023.51239672-73
TCGGAAC27000.022.18275870-71
GCGGTTA22750.020.79218170-71
GATCGTC22150.019.81653872-73
ATCGGTG26350.019.24054772-73
ATCGGTC17200.018.79346872-73
AGATCGA87900.018.66056670-71
GATCGTT24700.018.45948672-73
CGGCTCG21350.018.17078270-71
TCGGACC22350.017.96454672-73
TCGGACA27550.017.90845772-73
AGAGCGC92400.017.788670-71
CGGGTCG14200.017.73413570-71
Produced by FastQC (version 0.11.3)