FastQCFastQC Report
Sun 14 Aug 2016
V9M_H3K27ac_ChIPSeq.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameV9M_H3K27ac_ChIPSeq.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30537984
Sequences flagged as poor quality0
Sequence length36
%GC58

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTT723540.23693116087820337Illumina Paired End PCR Primer 2 (96% over 25bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN585520.1917349881380513No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGA121300.022.8051114
AATGCCG124650.022.25573313
CGGTTCA122850.022.1882021
AGCGGTT109500.021.06214130
CTCGTAT20150.020.82283228
TCTCGTA21400.019.40401327
TCGTATG21850.019.12350529
ATCGGAA122500.019.03866622
TGCCGAG148550.018.77825215
GAATGCC151050.018.50222812
GTTCAGC150550.018.3816493
AAGAGCG126600.018.3370127
GAGCGGT127300.018.2656929
TCGGAAG130850.017.96105423
TATGCCG25100.017.6572863
ACCGATC23450.017.54724122
CGGAAGA133400.017.52402924
GACCGAT23350.017.48313721
GATCGGA136250.017.19506321
GGTTCAG164650.016.8162172
Produced by FastQC (version 0.11.3)