##FastQC	0.11.3
>>Basic Statistics	pass
#Measure	Value
Filename	V8_input.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	30993375
Sequences flagged as poor quality	0
Sequence length	36
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.54416810044082	38.0	38.0	39.0	35.0	40.0
2	36.47348999584589	38.0	35.0	39.0	31.0	40.0
3	36.56160366529944	38.0	35.0	39.0	31.0	40.0
4	37.07530822312833	38.0	36.0	39.0	33.0	40.0
5	36.986918914122775	38.0	36.0	39.0	33.0	40.0
6	36.566239914175206	38.0	35.0	39.0	31.0	40.0
7	36.52555454189807	38.0	35.0	39.0	31.0	40.0
8	37.33890913783994	38.0	37.0	39.0	33.0	40.0
9	36.78390685106091	38.0	36.0	39.0	33.0	40.0
10	37.44289571561664	38.0	38.0	39.0	33.0	40.0
11	37.613620426946085	39.0	38.0	39.0	33.0	40.0
12	37.30170883293607	39.0	37.0	39.0	33.0	40.0
13	37.07375218090963	39.0	36.0	39.0	33.0	40.0
14	36.85263547451673	38.0	36.0	39.0	32.0	40.0
15	37.40987062557724	39.0	37.0	39.0	33.0	40.0
16	36.36939300737658	38.0	35.0	39.0	31.0	40.0
17	37.005223309820245	38.0	36.0	39.0	33.0	40.0
18	37.02020812512352	38.0	36.0	39.0	33.0	40.0
19	37.175367316402294	39.0	36.0	39.0	33.0	40.0
20	37.13665959257422	38.0	36.0	39.0	33.0	40.0
21	36.10262160865024	38.0	35.0	39.0	31.0	40.0
22	36.905009022089395	38.0	36.0	39.0	33.0	40.0
23	37.32678193323573	38.0	37.0	39.0	33.0	40.0
24	37.14886829846701	38.0	37.0	39.0	33.0	40.0
25	36.88071576586932	38.0	36.0	39.0	33.0	40.0
26	35.97237516082066	38.0	35.0	39.0	30.0	40.0
27	36.042449717076636	38.0	35.0	39.0	30.0	40.0
28	35.55276513125789	38.0	35.0	39.0	29.0	40.0
29	36.1726381525084	38.0	36.0	39.0	31.0	40.0
30	36.163624129350225	38.0	36.0	39.0	31.0	40.0
31	35.938820312405475	38.0	35.0	39.0	30.0	40.0
32	35.27494921092008	38.0	35.0	39.0	29.0	40.0
33	35.50986986735068	38.0	35.0	39.0	29.0	40.0
34	35.173598648098185	38.0	35.0	39.0	29.0	40.0
35	35.25615716261943	38.0	35.0	39.0	29.0	40.0
36	34.732608307420534	38.0	34.0	39.0	27.0	40.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1	1	-0.6673634335848391
1	2	-0.3963478846653956
1	3	-0.282530775769132
1	4	-0.2139810312020387
1	5	-0.08340957891454082
1	6	-0.33664202966696877
1	7	-0.1947887080490247
1	8	-0.1308866708331422
1	9	-0.22647631342246655
1	10	-0.2314879521452653
1	11	-0.2312827141059799
1	12	-0.4763881591498915
1	13	-0.26376635604838583
1	14	-0.19374998877687233
1	15	-0.39744342011873357
1	16	-0.2719885991425528
1	17	-0.15841728520227605
1	18	-0.19835259837513064
1	19	-0.3937275613526978
1	20	-0.3446270579660293
1	21	-0.6874347501500608
1	22	-0.46599218465065206
1	23	-0.3581266728444845
1	24	-0.1880672647207149
1	25	-0.28636018351046033
1	26	-1.049219185956055
1	27	-0.5921956133346455
1	28	-0.5812266973513687
1	29	-0.68534396437731
1	30	-1.0057163837055612
1	31	-0.5587060533494679
1	32	-0.5670729318757424
1	33	-0.8132625878964177
1	34	-1.0334319178147524
1	35	-0.834444260725796
1	36	-0.8066445527215862
2	1	-1.0367258485437318
2	2	-0.93851674484808
2	3	-0.46151012444577333
2	4	-0.4143367425490325
2	5	-0.5606700550770469
2	6	-1.0891840254394793
2	7	-0.27803058241742207
2	8	-0.5556605756668631
2	9	-0.9457088153900699
2	10	-0.857996556296321
2	11	-0.3163664802698918
2	12	-0.6215621106155922
2	13	-0.29340062016205337
2	14	-0.21357859684405867
2	15	-0.46225154296509885
2	16	-0.5389525906404131
2	17	-0.2878503994657322
2	18	-0.3272126013524286
2	19	-0.6228471183329063
2	20	-0.4923852896882721
2	21	0.2326862230644835
2	22	-0.34653726179499245
2	23	-0.6017755229214146
2	24	-0.4065209348083201
2	25	-0.2754142178967314
2	26	-1.0369980116445774
2	27	-0.5129058495289414
2	28	-0.507852217485798
2	29	-0.5689783546041411
2	30	-0.8518709638974684
2	31	-0.5082691505020591
2	32	-0.36986486535980134
2	33	-0.6738971753131509
2	34	-0.9084487111668551
2	35	-0.5317705130823995
2	36	-0.5366996733463765
3	1	-0.834922276716604
3	2	-1.0597691326348126
3	3	-0.5391362107447151
3	4	-0.7086172438065077
3	5	-0.7965834760076689
3	6	-0.9449375886375861
3	7	-0.5333659171454173
3	8	-0.6345883598874167
3	9	-0.9568882614501888
3	10	-0.7444606319914797
3	11	-0.34122894871991605
3	12	-0.8554986658410471
3	13	-0.34115448132190807
3	14	-0.44541258334751177
3	15	-0.5744116894781754
3	16	-0.761758221070032
3	17	-0.4143003735088442
3	18	-0.3237083697188581
3	19	-0.7325777845503936
3	20	-0.4242301878995889
3	21	0.0662266438218495
3	22	-0.644655231254859
3	23	-0.7274559989810641
3	24	-0.5424497377782274
3	25	-0.36643934189638117
3	26	-1.2127952574936884
3	27	-0.535346438022664
3	28	-0.5152867410316162
3	29	-0.5186345089185878
3	30	-0.8596371027572687
3	31	-0.4945576955370754
3	32	-0.43240321135062487
3	33	-0.6853463671452431
3	34	-0.7313430835588335
3	35	-0.4164255480036161
3	36	-0.43332313969748526
4	1	-0.9365702376025524
4	2	-0.9165071099245026
4	3	-0.8567379923434402
4	4	-0.516122087584769
4	5	-0.7516044628996639
4	6	-0.5900752630500108
4	7	-0.737592502771875
4	8	-0.5702986917123454
4	9	-0.8252301898827739
4	10	-0.9574560601316762
4	11	-0.43093863963188994
4	12	-1.0324107949234929
4	13	-0.4098043281211332
4	14	-0.3646154760871312
4	15	-0.5867288462135747
4	16	-0.897910649881112
4	17	-0.2243131558919913
4	18	-0.45951960787532187
4	19	-0.7149204163622613
4	20	-0.3756158086787593
4	21	0.10462238404043944
4	22	-0.6008909613395446
4	23	-0.7251362639269203
4	24	-0.3163273329006202
4	25	-0.2902779720647004
4	26	-0.9877429445829762
4	27	-0.3110305445420991
4	28	-0.4403475006137114
4	29	-0.5710286153050674
4	30	-0.8715776017872443
4	31	-0.46794237119650006
4	32	-0.5583447435018982
4	33	-0.8191117012039726
4	34	-0.7319661640646302
4	35	-0.492525401165544
4	36	-0.5902449362881299
5	1	-0.8094606376026974
5	2	-0.5029233056051865
5	3	-0.26145403278917456
5	4	-0.3099914998137834
5	5	-0.7463133134606466
5	6	-0.5568166011534714
5	7	-0.5342708360374999
5	8	-0.5194123220043281
5	9	-0.8419012642279782
5	10	-0.41376824971104753
5	11	-0.20083107484480678
5	12	-0.6570346567783787
5	13	-0.31887112917380733
5	14	-0.07205270237712114
5	15	-0.3754931253597249
5	16	-0.26758787411960583
5	17	-0.13475364120656508
5	18	-0.28521371066546664
5	19	-0.5407175993256672
5	20	-0.22046950599355597
5	21	0.31472093220225617
5	22	-0.5321025410892304
5	23	-0.564073224339424
5	24	-0.1836275501749327
5	25	-0.321767482210511
5	26	-1.0492726665899852
5	27	-0.2238546538125803
5	28	-0.16817539422386574
5	29	-0.48623868943315074
5	30	-0.7052326572294589
5	31	-0.24167944925815021
5	32	-0.21186285728973786
5	33	-0.5109251860908728
5	34	-0.7743272692546199
5	35	-0.25385237725815557
5	36	-0.28380724861833073
6	1	-0.4277392490934844
6	2	-0.3469638976872531
6	3	-0.4803179249790759
6	4	-0.17387804835191645
6	5	-0.949138585079389
6	6	-0.45011189080861413
6	7	-0.031028643157711144
6	8	-0.23348655031958998
6	9	-0.48561779585202913
6	10	-0.5024134752620739
6	11	-0.14650855557984244
6	12	-0.7191707552844591
6	13	-0.5717055092906094
6	14	-0.31546073793792573
6	15	-0.48069970704599996
6	16	-0.4122090748228189
6	17	-0.20960454950396468
6	18	-0.19053985352059755
6	19	-0.29757512650784435
6	20	-0.14498176327116852
6	21	0.33059741451494773
6	22	-0.3583010321783249
6	23	-0.40805062781348056
6	24	-0.25264458721031957
6	25	-0.20041036363210907
6	26	-0.8347327105913038
6	27	-0.18078391819781103
6	28	-0.18985955731922388
6	29	-0.32185568706302803
6	30	-0.479175394035515
6	31	-0.10350980057241799
6	32	-0.13244948721246175
6	33	-0.5873406305876898
6	34	-0.38249696406845857
6	35	-0.1391971740564273
6	36	-0.29642838323072596
7	1	-0.423319826422933
7	2	-0.13398609965848607
7	3	-0.2628595024992677
7	4	-0.303150699690498
7	5	-0.33812058270354584
7	6	-0.01051728951412656
7	7	0.048496387293511134
7	8	-0.16462433480938898
7	9	-0.47071937323555346
7	10	-0.19385514882304733
7	11	-0.09610792571817939
7	12	-0.5071742434551609
7	13	-0.17691015939285393
7	14	-0.031777430945240326
7	15	-0.23702617923095914
7	16	-0.07581207903526632
7	17	0.06634696879024204
7	18	-0.15926730289341862
7	19	-0.2612486325810295
7	20	-0.053196790413352346
7	21	0.031176177369772518
7	22	-0.3631125894673275
7	23	-0.3687542937665853
7	24	-0.024009671966489066
7	25	-0.13977526451427025
7	26	-0.39360992681957185
7	27	0.002620250500847021
7	28	-0.028416932163665365
7	29	-0.20511841176757173
7	30	-0.33965980964559606
7	31	-0.03284760294489786
7	32	-0.21906559551518967
7	33	-0.6745366346514032
7	34	-0.24462331090383316
7	35	-0.009613292663239292
7	36	0.01743793857899334
8	1	-0.6587934818761241
8	2	-0.16450099970457188
8	3	-0.03563502413510378
8	4	-0.13993075641892005
8	5	-0.3683249188504547
8	6	-0.18261850523037992
8	7	-0.20757806697108805
8	8	-0.4694863753287777
8	9	-0.7488502857929191
8	10	-0.42745251373356297
8	11	-0.25800603818168355
8	12	-0.6941639656149619
8	13	-0.29601007016864145
8	14	-0.08010646317073622
8	15	-0.5098333324033746
8	16	-0.4538617230638451
8	17	-0.22043288602500155
8	18	-0.2314117570953016
8	19	-0.45382594582930125
8	20	-0.2729049157212913
8	21	0.2624369982723209
8	22	-0.36920796010269186
8	23	-0.3952419052452285
8	24	-0.13421490142629722
8	25	-0.3198096570607376
8	26	-0.431093563820653
8	27	-0.17697321870884508
8	28	-0.17753982127217682
8	29	-0.38286292825661405
8	30	-0.48355842138535365
8	31	-0.12902266198538115
8	32	-0.10156949955258199
8	33	-0.5615891518128748
8	34	-0.4016198919934766
8	35	-0.20443963584865799
8	36	-0.03476929073916324
9	1	-0.5522237425525987
9	2	-0.17084214434384393
9	3	-0.14049919284705936
9	4	-0.10905171256597157
9	5	-0.2946550841415032
9	6	-0.10957209438151949
9	7	0.07559638889156162
9	8	-0.44338792088955614
9	9	-0.5137137971930699
9	10	-0.4661708266459712
9	11	-0.18578474398725575
9	12	-0.7247261873300701
9	13	-0.571795557000442
9	14	-0.1626504538626392
9	15	-0.4537881699557147
9	16	-0.4429246994594749
9	17	-0.09483987089463852
9	18	-0.178007549728747
9	19	-0.4692288059676244
9	20	-0.18292309049997613
9	21	0.3061643970390122
9	22	-0.34649221300888655
9	23	-0.338116330661542
9	24	-0.1288675041584355
9	25	-0.26942931729654873
9	26	-0.33653146810309664
9	27	-0.15370325430591691
9	28	-0.1453464101766997
9	29	-0.2672649561172378
9	30	-0.368123252883116
9	31	-0.0828586949585457
9	32	-0.0776521214044763
9	33	-0.4990024168193443
9	34	-0.24710713641439241
9	35	-0.13720726912799108
9	36	-0.03448352896314333
10	1	-0.32783931129214494
10	2	-0.059435537357337864
10	3	-0.05061292083566826
10	4	-0.029550587864356714
10	5	-0.29671368118394525
10	6	-0.19956131445889014
10	7	0.14133247663194481
10	8	-0.1722222146120771
10	9	-0.27198796388268676
10	10	-0.16951487250557307
10	11	-0.0482598402736798
10	12	-0.7834548271580033
10	13	0.04409806105593361
10	14	0.08945741968843635
10	15	-0.24263936016483
10	16	-0.07653940800005188
10	17	-0.023004957036334872
10	18	-0.06954157700631924
10	19	-0.2965444523280354
10	20	-0.012761582064158006
10	21	0.3455614438035113
10	22	-0.055553539963561605
10	23	-0.1655891855343441
10	24	0.11743565567955017
10	25	-0.2101318067912672
10	26	-0.23177799142097655
10	27	0.08138223207004813
10	28	0.046079205644204535
10	29	-0.23502243948739476
10	30	-0.2827461248344747
10	31	-6.734360253943805E-4
10	32	0.027684391434995348
10	33	-0.34430278464964204
10	34	-0.13167446583241116
10	35	-0.012757489163966795
10	36	-0.06533854180089094
11	1	-0.24955165085300024
11	2	-0.11287690494636848
11	3	-0.24001684670748347
11	4	-0.0018054610040039165
11	5	-0.5509029520264974
11	6	-0.3001946198955139
11	7	0.03372384226504721
11	8	-0.18913914628470252
11	9	-0.36426931101598825
11	10	-0.16259286534844364
11	11	0.03142090638809947
11	12	-0.6729687029910707
11	13	-0.06873558430179116
11	14	-0.05995685559503272
11	15	-0.3095954394289393
11	16	0.03906947706899189
11	17	-0.05206466384979791
11	18	-0.05207522454283975
11	19	-0.24422271432010234
11	20	-0.01098170896626982
11	21	0.2378132917033966
11	22	-0.12564615585782235
11	23	-0.21163963745114955
11	24	0.10726314794294467
11	25	-0.09694126253140212
11	26	-0.20584257396175332
11	27	-0.06901268087808887
11	28	0.06558545216447698
11	29	-0.1950174125573625
11	30	-0.24783405745644416
11	31	0.0032694528009855617
11	32	0.019566581169328856
11	33	-0.4408791157344325
11	34	-0.06691744658144216
11	35	0.038669079202257706
11	36	0.07408490152372593
12	1	-0.2841042476112605
12	2	0.03961730938605257
12	3	0.006108535666015769
12	4	0.006074290966175511
12	5	-0.4562532821219989
12	6	-0.292026701034672
12	7	0.1260378761163068
12	8	-0.2383333029422161
12	9	-0.3943071699236711
12	10	-0.4535193768534569
12	11	-0.07072086077744189
12	12	-0.9384285511100785
12	13	-0.04544493739496147
12	14	-0.0581250346540827
12	15	-0.269151911433525
12	16	0.08232185407272397
12	17	0.032859690698330724
12	18	-0.018231151018447633
12	19	-0.18599726089345836
12	20	0.04707855214447676
12	21	0.2562877478317489
12	22	-0.07793262786820776
12	23	-0.20719134347896784
12	24	0.015341433290700479
12	25	-0.19038665425523504
12	26	-0.29655233821464577
12	27	0.11473245639691498
12	28	0.014927430705348854
12	29	-0.13707148621772092
12	30	-0.21462433629987743
12	31	0.050825141207781144
12	32	0.09325274646714377
12	33	-0.3798146264735536
12	34	-0.05592902661572907
12	35	0.16889252684347866
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115	24	-0.136322987513239
115	25	-0.10885017844056932
115	26	-0.6710590025948875
115	27	-0.36273249435931376
115	28	-0.3941615121521025
115	29	-0.46519813526439435
115	30	-0.772404681404403
115	31	-0.4225401724005948
115	32	-0.35958886797214973
115	33	-0.6671450602602533
115	34	-0.7953062116233127
115	35	-0.4560594266624065
115	36	-0.49674699394692823
116	1	-0.34433620453451397
116	2	-0.27798663037552274
116	3	-0.3172098148833271
116	4	-0.05619851338190074
116	5	-0.07385848719668786
116	6	-0.29487494085538657
116	7	-0.08480650408344559
116	8	-0.1298289109995352
116	9	-0.3340859676580976
116	10	-0.22778059602865852
116	11	-0.07839746116244584
116	12	-0.31450597202537267
116	13	-0.40154178327739487
116	14	-0.18848103571557573
116	15	-0.20659543427778715
116	16	-0.4705682908427278
116	17	-0.1818391346344015
116	18	-0.13761485795070172
116	19	-0.3848023242928065
116	20	-0.2696095461858192
116	21	0.1376023077759072
116	22	-0.1388071539981155
116	23	-0.2404827677594099
116	24	-0.23956797330590263
116	25	-0.03797041494264164
116	26	-0.6696502756386948
116	27	-0.4881910936626781
116	28	-0.29649472836550217
116	29	-0.42979195766817213
116	30	-0.6861860383783451
116	31	-0.35114322137873444
116	32	-0.37627153090812726
116	33	-0.42848373736934775
116	34	-0.8006576204292841
116	35	-0.4467200079881337
116	36	-0.4182413128553293
117	1	-0.5702067164287428
117	2	-0.4003597623267581
117	3	-0.6873921762321089
117	4	-0.37947402870180014
117	5	-0.5583412085254267
117	6	-0.35288570361114324
117	7	-0.20693593475531458
117	8	-0.3824305486471431
117	9	-0.4891948255555931
117	10	-0.3163110042585089
117	11	-0.2909644775567699
117	12	-0.6710692623115975
117	13	-0.3537868015500436
117	14	-0.14651657905960036
117	15	-0.4607005074756074
117	16	-0.8011063860715311
117	17	-0.29284350785951574
117	18	-0.33103161224077127
117	19	-0.5705473945122179
117	20	-0.39932251537005214
117	21	0.20669935842450116
117	22	-0.28897190128221695
117	23	-0.7016971535620442
117	24	-0.2911078462679768
117	25	-0.10435942266112619
117	26	-0.9903202076447855
117	27	-0.63155980049617
117	28	-0.5524079799658779
117	29	-0.608546276270971
117	30	-1.093144796020944
117	31	-0.5980030284036317
117	32	-0.5440777911103112
117	33	-0.7542703496169025
117	34	-1.0896518482798427
117	35	-0.6891223559977959
117	36	-0.6857221875345516
118	1	-0.5400881471022103
118	2	-0.3019523906244501
118	3	-0.21879959384096992
118	4	-0.11807972576796288
118	5	-0.2155923274521001
118	6	-0.3962727161382418
118	7	-0.25448191311654966
118	8	-0.3209743602124604
118	9	-0.3822475043133906
118	10	-0.14297378526732274
118	11	-0.20455324623645055
118	12	-0.4288796046615104
118	13	-0.36254264551109827
118	14	-0.25063124954270677
118	15	-0.3581624598385673
118	16	-0.5262770091994824
118	17	-0.21320197741035685
118	18	-0.38060119220221367
118	19	-0.5027677247410125
118	20	-0.34547838047440393
118	21	-0.19095916553283843
118	22	-0.2654284186384004
118	23	-0.3980456423193175
118	24	-0.35995167123641636
118	25	-0.09300655102624233
118	26	-0.9789968599861112
118	27	-0.5917289279892941
118	28	-0.5505422490905403
118	29	-0.6270355573227775
118	30	-0.8883515334817673
118	31	-0.6050899394691882
118	32	-0.5336977833577734
118	33	-0.7023262264281414
118	34	-0.9281497047022356
118	35	-0.7262849363462749
118	36	-0.5901693597212656
119	1	-0.5551940338543844
119	2	-0.46653598377145045
119	3	-0.4543040106240568
119	4	-0.3381634092463841
119	5	-0.45084628878552735
119	6	-0.4886765427231836
119	7	-0.4026905467255304
119	8	-0.31893564792681417
119	9	-0.5826534178136455
119	10	-0.29473277879334603
119	11	-0.2513822257658589
119	12	-0.7114971460062804
119	13	-0.496624721608967
119	14	-0.2380596994035784
119	15	-0.4517664531770933
119	16	-0.39376890317724644
119	17	-0.26605695938708607
119	18	-0.3465153793845559
119	19	-0.46640105165821666
119	20	-0.4093143768280427
119	21	-0.14711243645309935
119	22	-0.3349610902768063
119	23	-0.43165219948105005
119	24	-0.43454263997215037
119	25	-0.10963513467350339
119	26	-1.0897310538420086
119	27	-0.754568806131509
119	28	-0.7671416712189725
119	29	-0.8263920206232314
119	30	-0.9981175787036918
119	31	-0.7231800698071567
119	32	-0.6980964614126961
119	33	-0.9263476015160705
119	34	-1.1106321527908918
119	35	-0.8793013951849957
119	36	-0.8709711770411133
120	1	-0.6666932251517963
120	2	-0.6058353864891473
120	3	-0.4602134955110202
120	4	-0.4267668350718168
120	5	-0.5344092898923947
120	6	-0.6414630899394353
120	7	-0.29611146798455223
120	8	-0.3977673753572546
120	9	-0.2856323519852424
120	10	-0.3775786589640404
120	11	-0.33067120657894833
120	12	-0.7253138937749313
120	13	-0.4916271575734257
120	14	-0.08214070372685711
120	15	-0.6623083878510627
120	16	-0.6226488965223851
120	17	-0.3005197695292594
120	18	-0.47516058182417
120	19	-0.5898409605776322
120	20	-0.6133101796547322
120	21	-0.17297992072528956
120	22	-0.4848193586877514
120	23	-0.5807074072983553
120	24	-0.3997274654802254
120	25	-0.38804751083047506
120	26	-1.4373879572732733
120	27	-0.9929799155265115
120	28	-1.0375688880364677
120	29	-1.0177967316948795
120	30	-1.2142958485170752
120	31	-0.8668982872043998
120	32	-0.9738600137759619
120	33	-1.2064972416357804
120	34	-1.3806727838788078
120	35	-1.1729760869499373
120	36	-1.1470972778889958
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	21447.0
3	2039.0
4	901.0
5	594.0
6	433.0
7	399.0
8	771.0
9	1681.0
10	2164.0
11	1616.0
12	2169.0
13	2902.0
14	3819.0
15	3067.0
16	3903.0
17	5373.0
18	8014.0
19	9071.0
20	11434.0
21	17414.0
22	25533.0
23	35324.0
24	51603.0
25	80134.0
26	126653.0
27	168425.0
28	210771.0
29	292520.0
30	444458.0
31	634119.0
32	875467.0
33	1236351.0
34	1798294.0
35	2697961.0
36	4260800.0
37	6879157.0
38	7038426.0
39	4037908.0
40	260.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	84.74064902758568	4.428898668826616	3.7848423775806603	7.04560992600706
2	7.139122228227114	45.169932162648315	3.77204713929646	43.91889846982811
3	47.456307098513086	44.689198629745384	4.546279648766103	3.3082146229754215
4	44.56423572914075	3.775035490676694	47.375558067277076	4.2851707129054875
5	6.5413601760894435	4.19431077773483	84.48235653208076	4.781972514094964
6	44.59970000654657	7.726042976390124	3.0083847360383165	44.665872281024996
7	5.0346598264951785	85.14758073297922	3.3973744388921827	6.420385001633414
8	44.83550530733267	4.845024182434071	3.71215479235772	46.607315717875544
9	4.500874770724375	44.03558140891518	4.491821190565795	46.971722629794655
10	6.911332686096046	4.647163722963081	43.99367346523939	44.44783012570148
11	47.97292383420809	4.013856538717548	4.353755697600547	43.659463929473816
12	50.29485223606724	5.067030409551787	40.531961708782354	4.106155645598611
13	43.98997939492674	10.638876651492007	4.473454908277386	40.89768904530386
14	7.007465305085361	47.40324020859297	4.059880538986154	41.52941394733552
15	44.3673610538849	7.550498683798973	7.964724663832004	40.11741559848412
16	41.956991496145996	43.9997387827502	10.283777244021856	3.7594924770819453
17	40.96831358730255	5.120748808489814	44.2558630774085	9.655074526799135
18	40.248822857173835	7.9667498390687745	8.342159773675734	43.442267530081665
19	10.011365020091732	4.958067450994159	80.36518548207088	4.665382046843216
20	43.454479696460965	11.739865687939014	5.257953775622045	39.54770083997798
21	3.8566728442238283	83.28088394896548	7.23983801311332	5.6226051936973755
22	3.743948696041598	45.59661243132391	7.638239465794565	43.021199406839926
23	6.004441159285637	4.8904957042433	49.51370554480896	39.59135759166211
24	38.82671617066891	6.424655584643904	44.027333761834285	10.721294482852901
25	5.234896232277599	11.024867987426944	5.1004734577775945	78.63976232251785
26	14.217193937682524	69.54027735546423	6.546573813898335	9.695954892954916
27	68.71252793908602	16.321792061182435	8.579816151198758	6.385863848532789
28	11.185785943740465	65.67117538877037	15.149014590018101	7.994024077471074
29	10.013107433621414	10.163021910867839	65.37659938326044	14.447271272250298
30	14.000853946304117	9.021575195689575	12.619642746113982	64.35792811189232
31	73.76784699887	9.671569339502376	7.008905809971572	9.551677851656047
32	66.1818638926924	19.65954362537719	7.386247225280417	6.772345256649986
33	9.50848316096372	73.61157169479502	7.236816675304023	9.643128468937237
34	13.448744988119401	66.5891965186383	10.128459802118444	9.833598691123854
35	68.48025945911338	14.217944157894122	9.447628348867978	7.854168034124519
36	15.140170776242387	65.3988958670714	7.148785093676281	12.31214826300992
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	577.0
1	339.5
2	102.0
3	102.0
4	131.0
5	160.0
6	160.0
7	266.0
8	372.0
9	372.0
10	769.0
11	1166.0
12	1166.0
13	2317.0
14	3468.0
15	6160.5
16	8853.0
17	8853.0
18	14158.0
19	19463.0
20	19463.0
21	28995.0
22	38527.0
23	38527.0
24	53210.0
25	67893.0
26	89252.5
27	110612.0
28	110612.0
29	138817.5
30	167023.0
31	167023.0
32	198517.0
33	230011.0
34	230011.0
35	264182.5
36	298354.0
37	298354.0
38	413182.5
39	528011.0
40	448211.0
41	368411.0
42	368411.0
43	412446.0
44	456481.0
45	456481.0
46	541606.0
47	626731.0
48	626731.0
49	850780.5
50	1074830.0
51	2068127.5
52	3061425.0
53	3061425.0
54	1.1708614E7
55	2.0355803E7
56	2.0355803E7
57	1.11934745E7
58	2031146.0
59	2031146.0
60	1453653.5
61	876161.0
62	876161.0
63	638130.0
64	400099.0
65	276199.5
66	152300.0
67	152300.0
68	106391.0
69	60482.0
70	60482.0
71	43959.0
72	27436.0
73	27436.0
74	20555.5
75	13675.0
76	10273.0
77	6871.0
78	6871.0
79	5157.5
80	3444.0
81	3444.0
82	2603.0
83	1762.0
84	1762.0
85	1298.0
86	834.0
87	834.0
88	597.5
89	361.0
90	257.0
91	153.0
92	153.0
93	122.5
94	92.0
95	92.0
96	79.5
97	67.0
98	67.0
99	143.0
100	219.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.37461231634179887
2	4.033119981286323E-4
3	3.549145583531964E-5
4	7.74359036406974E-5
5	1.1292735947601704E-4
6	1.0324787152092987E-4
7	0.0
8	7.001496287513057E-4
9	3.581410543382255E-4
10	3.3878207842805115E-4
11	2.0972223902688883E-4
12	1.7423078319156918E-4
13	1.1938035144607518E-4
14	0.0
15	0.0012551069381763038
16	5.1623935760464934E-5
17	0.001819743735556389
18	0.001038931707179357
19	9.35683835658427E-5
20	0.012109039431814057
21	6.452991970058117E-6
22	1.7745727917659824E-4
23	3.742735342633708E-4
24	1.8068377516162727E-4
25	3.7104703827834176E-4
26	2.548931828172956E-4
27	0.003068397681762635
28	0.0010841026509697638
29	0.003349102832460163
30	0.0025618378121130725
31	0.0011260470987751415
32	4.936538857094459E-4
33	3.0006412660770246E-4
34	0.008108184410378024
35	0.0168326295538966
36	0.007030534751378319
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
36	3.0993375E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.904505177079578
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.62487079689757	17.043206840636177
2	7.174750918273299	2.8561973359405464
3	3.038324379292768	1.8142903001184003
4	1.5296731284303893	1.2178954681632879
5	0.8562547454323542	0.8521663506678626
6	0.48394659349237695	0.5779630485359427
7	0.30299879235761035	0.42217287217916405
8	0.1931656202403388	0.30758928704860894
9	0.1395107221550798	0.2499202702254507
>10	0.5675659222048611	1.9768628206742966
>50	0.04277535391310288	0.5979321064209062
>100	0.03494562591988269	1.4027057037622608
>500	0.004007394316398309	0.5479689791842604
>1k	0.004960868892855905	2.271420305893632
>5k	9.411287425232893E-4	1.2924865822689198
>10k+	0.0013080094387611816	66.56922172828021
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATTCAGATCTCCCGGGTCACCGCAGATCGGAAGA	9521742	30.72186233348256	No Hit
GCGGTGACCCGGGAGATCTGAATTCAGATCGGAAGA	7030714	22.684570492887595	No Hit
GCGGTGACCCGGGAGATCTGAATTCGATCTGAATTC	626618	2.021780461146939	No Hit
GAATTCAGATCGAATTCAGATCTCCCGGGTCACCGC	588949	1.9002415838868791	No Hit
CGGTGACCCGGGAGATCTGAATTCAGATCGGAAGAG	541212	1.746218345049547	No Hit
GAATTCAGATCTCCCGGGTCACCGAGATCGGAAGAG	485080	1.5651086724178958	No Hit
GAATTCAGATCGAATTCAGATCGAATTCAGATCTCC	176562	0.5696765841087007	No Hit
TGACCCGGGAGATCTGAATTCAGATCGGAAGAGCGG	148946	0.4805736709861382	No Hit
AATTCAGATCTCCCGGGTCACCGCAGATCGGAAGAG	144960	0.4677128579898123	No Hit
GAATTCAGATCTCCCGGGTCAAGATCGGAAGAGCGG	116173	0.3748317180687808	No Hit
GCGGTGACCCGGGAGATCTGAATTAGATCGGAAGAG	82593	0.26648598289150505	No Hit
GCGGTGACCCGGGAGATCTGAATTCAAGATCGGAAG	52605	0.16972982129245365	No Hit
GTGACCCGGGAGATCTGAATTCAGATCGGAAGAGCG	50049	0.16148289755471937	No Hit
ATTCAGATCTCCCGGGTCACCGCAGATCGGAAGAGC	46259	0.14925447777145923	No Hit
GAATTCAGATCTCCCGGATCACCGCAGATCGGAAGA	43251	0.13954917784849183	No Hit
GAATTCAGATCTCCCGGTCACCGCAGATCGGAAGAG	41799	0.13486430567822963	No Hit
GAATTCAGATCTCCCGAGTCACCGCAGATCGGAAGA	41482	0.1338415064509754	No Hit
GCGGTGACCGGGAGATCTGAATTCAGATCGGAAGAG	39538	0.12756919825607893	No Hit
CGGTGACCCGGGAGATCTGAATTCGATCTGAATTCA	39044	0.12597530923947456	No Hit
GAATTCAGATCTCCCGGGTCACAGATCGGAAGAGCG	38375	0.12381678342549012	No Hit
NAATTCAGATCTCCCGGGTCACCGCAGATCGGAAGA	36859	0.11892541551218608	No Hit
GAATTCAGATCTCCGGGTCACCGCAGATCGGAAGAG	35420	0.11428248778972926	No Hit
GAATTCAGATCTCCCAGGTCACCGCAGATCGGAAGA	35267	0.11378883390401981	No Hit
GGTGACCCGGGAGATCTGAATTCAGATCGGAAGAGC	34784	0.11223043634325078	No Hit
GAATTCAGATCGAATTCAGATCTCCCGGGTCACCGA	34491	0.11128507301963726	No Hit
GCGGTGACCCGGAGATCTGAATTCAGATCGGAAGAG	32833	0.10593554267645908	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.1299148608371953E-4	0.0	0.0	0.0
2	5.001068776795041E-4	0.0	0.0	3.226495985029059E-6
3	7.227351006465091E-4	0.0	0.0	3.226495985029059E-6
4	9.163248597482527E-4	0.0	0.0	3.226495985029059E-6
5	0.0011486325706703449	0.0	3.226495985029059E-6	3.226495985029059E-6
6	0.0015132266169786285	0.0	3.226495985029059E-6	3.226495985029059E-6
7	0.031403485422287825	0.0	3.226495985029059E-6	3.226495985029059E-6
8	0.03166483159707518	0.0	3.226495985029059E-6	3.226495985029059E-6
9	0.03182292990034161	0.0	3.226495985029059E-6	3.226495985029059E-6
10	0.03217139146672474	0.0	3.226495985029059E-6	3.226495985029059E-6
11	0.03252630602507794	0.0	3.226495985029059E-6	3.226495985029059E-6
12	0.03306835735056282	0.0	3.226495985029059E-6	3.226495985029059E-6
13	0.03358459670816747	0.0	3.226495985029059E-6	3.226495985029059E-6
14	0.03675301576546601	0.0	3.226495985029059E-6	3.226495985029059E-6
15	0.043580281269787496	3.226495985029059E-6	3.226495985029059E-6	3.226495985029059E-6
16	0.044951542063424846	3.226495985029059E-6	3.226495985029059E-6	3.226495985029059E-6
17	0.048449063711196345	3.226495985029059E-6	6.452991970058118E-6	3.226495985029059E-6
18	0.06178417161732144	6.452991970058118E-6	6.452991970058118E-6	3.226495985029059E-6
19	0.14901894356455211	6.452991970058118E-6	9.679487955087176E-6	3.226495985029059E-6
20	0.3785066969957289	9.679487955087176E-6	9.679487955087176E-6	3.226495985029059E-6
21	0.5658596393584113	9.679487955087176E-6	9.679487955087176E-6	3.226495985029059E-6
22	1.492073709300778	9.679487955087176E-6	9.679487955087176E-6	3.226495985029059E-6
23	1.9018612848713636	9.679487955087176E-6	9.679487955087176E-6	3.226495985029059E-6
24	2.6376959592170905	9.679487955087176E-6	9.679487955087176E-6	3.226495985029059E-6
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGGTGA	1245535	0.0	30.055231	1
GCGGCGA	2645	0.0	29.025263	1
GCGTGAC	2365	0.0	28.70629	1
CCGCGAT	1310	0.0	28.507368	22
ACCGCAG	1064785	0.0	28.467354	21
CCGCAGA	1065125	0.0	28.434563	22
CGCAGAT	1064560	0.0	28.401047	23
CACCGCA	1072665	0.0	28.379969	20
GCAGATC	1064040	0.0	28.375782	24
ACCGAGA	56500	0.0	28.1534	21
ACCGCGA	1695	0.0	28.137472	21
CACCGAG	57460	0.0	28.116018	20
CGGTGAC	1331340	0.0	28.05051	2
CGCCGAT	2285	0.0	28.026756	23
GCGGTTA	785	0.0	27.997217	1
ACCGCCG	2535	0.0	27.865744	21
GGTGACC	1343660	0.0	27.795034	3
CGGTTCG	395	0.0	27.71795	25
GCGATCG	1030	0.0	27.666256	24
CCGCAAG	3405	0.0	27.66124	22
>>END_MODULE
