FastQCFastQC Report
Sun 14 Aug 2016
V8_input.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameV8_input.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30993375
Sequences flagged as poor quality0
Sequence length36
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATTCAGATCTCCCGGGTCACCGCAGATCGGAAGA952174230.72186233348256No Hit
GCGGTGACCCGGGAGATCTGAATTCAGATCGGAAGA703071422.684570492887595No Hit
GCGGTGACCCGGGAGATCTGAATTCGATCTGAATTC6266182.021780461146939No Hit
GAATTCAGATCGAATTCAGATCTCCCGGGTCACCGC5889491.9002415838868791No Hit
CGGTGACCCGGGAGATCTGAATTCAGATCGGAAGAG5412121.746218345049547No Hit
GAATTCAGATCTCCCGGGTCACCGAGATCGGAAGAG4850801.5651086724178958No Hit
GAATTCAGATCGAATTCAGATCGAATTCAGATCTCC1765620.5696765841087007No Hit
TGACCCGGGAGATCTGAATTCAGATCGGAAGAGCGG1489460.4805736709861382No Hit
AATTCAGATCTCCCGGGTCACCGCAGATCGGAAGAG1449600.4677128579898123No Hit
GAATTCAGATCTCCCGGGTCAAGATCGGAAGAGCGG1161730.3748317180687808No Hit
GCGGTGACCCGGGAGATCTGAATTAGATCGGAAGAG825930.26648598289150505No Hit
GCGGTGACCCGGGAGATCTGAATTCAAGATCGGAAG526050.16972982129245365No Hit
GTGACCCGGGAGATCTGAATTCAGATCGGAAGAGCG500490.16148289755471937No Hit
ATTCAGATCTCCCGGGTCACCGCAGATCGGAAGAGC462590.14925447777145923No Hit
GAATTCAGATCTCCCGGATCACCGCAGATCGGAAGA432510.13954917784849183No Hit
GAATTCAGATCTCCCGGTCACCGCAGATCGGAAGAG417990.13486430567822963No Hit
GAATTCAGATCTCCCGAGTCACCGCAGATCGGAAGA414820.1338415064509754No Hit
GCGGTGACCGGGAGATCTGAATTCAGATCGGAAGAG395380.12756919825607893No Hit
CGGTGACCCGGGAGATCTGAATTCGATCTGAATTCA390440.12597530923947456No Hit
GAATTCAGATCTCCCGGGTCACAGATCGGAAGAGCG383750.12381678342549012No Hit
NAATTCAGATCTCCCGGGTCACCGCAGATCGGAAGA368590.11892541551218608No Hit
GAATTCAGATCTCCGGGTCACCGCAGATCGGAAGAG354200.11428248778972926No Hit
GAATTCAGATCTCCCAGGTCACCGCAGATCGGAAGA352670.11378883390401981No Hit
GGTGACCCGGGAGATCTGAATTCAGATCGGAAGAGC347840.11223043634325078No Hit
GAATTCAGATCGAATTCAGATCTCCCGGGTCACCGA344910.11128507301963726No Hit
GCGGTGACCCGGAGATCTGAATTCAGATCGGAAGAG328330.10593554267645908No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGTGA12455350.030.0552311
GCGGCGA26450.029.0252631
GCGTGAC23650.028.706291
CCGCGAT13100.028.50736822
ACCGCAG10647850.028.46735421
CCGCAGA10651250.028.43456322
CGCAGAT10645600.028.40104723
CACCGCA10726650.028.37996920
GCAGATC10640400.028.37578224
ACCGAGA565000.028.153421
ACCGCGA16950.028.13747221
CACCGAG574600.028.11601820
CGGTGAC13313400.028.050512
CGCCGAT22850.028.02675623
GCGGTTA7850.027.9972171
ACCGCCG25350.027.86574421
GGTGACC13436600.027.7950343
CGGTTCG3950.027.7179525
GCGATCG10300.027.66625624
CCGCAAG34050.027.6612422
Produced by FastQC (version 0.11.3)