FastQCFastQC Report
Sun 14 Aug 2016
V8_H3K4me1_ChIPSeq.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameV8_H3K4me1_ChIPSeq.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10568059
Sequences flagged as poor quality0
Sequence length71
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG7800.022.54204663
CTCGTAT9750.022.03827359
TCGTATG10050.021.38051660
TCTCGTA10650.019.86933558
CGTATGC8750.017.8607861
ATGCCGT11300.014.98169964
ATCTCGT14000.013.718883557
CCGATCT13850.012.92828254
ACCGATC14250.012.79488153
GACCGAT15100.012.50569952
TGCCGTC13850.012.45792565
TATGGCG5302.3646862E-1111.67271563
AACGCCG2551.9289744E-411.49048964
ACGAGCG28000.011.394365
TCCGCCG7150.011.38335164
CCGAGAC48950.011.30617965
ATGCCGA100700.011.05646465
CGGACGC9650.010.79548765
ACGCGCG7300.010.70423461
TGCCGAG92650.010.61160765
Produced by FastQC (version 0.11.3)