FastQCFastQC Report
Sun 14 Aug 2016
V703_input.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameV703_input.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22858863
Sequences flagged as poor quality0
Sequence length36
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGA16900.012.15789614
CGGTTCA17400.011.2530021
AATGCCG18700.011.06810913
GAGCGGT17900.010.9766429
TGCCGAG19200.010.62343815
AGCGGTT18700.010.58718830
AGAGCGG20500.09.51131628
TCGGAAG21750.09.10201123
ATCGGAA21750.09.03291722
GATCGGA22550.08.7790821
AAGAGCG23150.08.681684527
GCGGTTC9700.08.50397811
AGATCGG24850.07.90617820
CGGAAGA25200.07.855901724
CGAGATC36350.06.02387318
TAGATCG6451.1791417E-75.580879729
CCGAGAT43600.05.36618317
GCCGAGA46150.05.23215516
GAATGCC44050.05.07307812
GGTTCAG45900.05.0646592
Produced by FastQC (version 0.11.3)