##FastQC	0.11.3
>>Basic Statistics	pass
#Measure	Value
Filename	V503_input.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	29748713
Sequences flagged as poor quality	0
Sequence length	36
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.158215415907236	39.0	38.0	40.0	35.0	40.0
2	37.35618139177987	38.0	37.0	40.0	33.0	40.0
3	37.190132393290426	38.0	36.0	40.0	33.0	40.0
4	37.62505998158643	39.0	38.0	40.0	34.0	40.0
5	37.90489067543863	39.0	38.0	40.0	35.0	40.0
6	36.99458753055973	38.0	36.0	40.0	33.0	40.0
7	35.78661234857454	37.0	33.0	39.0	31.0	40.0
8	37.43003416652008	39.0	37.0	40.0	33.0	40.0
9	36.97006297381672	38.0	36.0	40.0	33.0	40.0
10	38.066718819062864	39.0	38.0	40.0	35.0	40.0
11	37.73408664771481	39.0	38.0	40.0	34.0	40.0
12	37.662864776704794	39.0	38.0	40.0	33.0	40.0
13	37.841462015516434	39.0	38.0	40.0	35.0	40.0
14	36.02656417438966	38.0	34.0	39.0	30.0	40.0
15	37.93407392111383	39.0	38.0	40.0	35.0	40.0
16	36.675323164400425	38.0	35.0	40.0	31.0	40.0
17	37.65153117716387	39.0	38.0	40.0	33.0	40.0
18	37.61083358463272	39.0	38.0	40.0	33.0	40.0
19	37.57240052704129	39.0	37.0	40.0	33.0	40.0
20	37.69892872340393	39.0	38.0	40.0	33.0	40.0
21	36.66936653696582	38.0	35.0	39.0	31.0	40.0
22	36.92413241540903	38.0	35.0	40.0	31.0	40.0
23	37.80877828227393	39.0	38.0	40.0	34.0	40.0
24	37.7408685881638	39.0	38.0	40.0	33.0	40.0
25	37.57185465468708	39.0	38.0	40.0	33.0	40.0
26	36.845872223110966	39.0	36.0	40.0	31.0	40.0
27	36.822848907783005	39.0	36.0	40.0	31.0	40.0
28	36.403874984440506	39.0	36.0	40.0	31.0	40.0
29	36.72290892046321	39.0	37.0	40.0	31.0	40.0
30	36.78020181915097	39.0	37.0	40.0	31.0	40.0
31	36.66414627752132	39.0	36.0	40.0	31.0	40.0
32	36.09868090764128	39.0	35.0	40.0	30.0	40.0
33	36.380524125531075	39.0	36.0	40.0	31.0	40.0
34	35.96255027906585	39.0	35.0	40.0	30.0	40.0
35	35.84889299244643	39.0	35.0	40.0	30.0	40.0
36	35.512340315360866	39.0	35.0	40.0	29.0	40.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
4	1	-5.398440313756723
4	2	-9.080853340168431
4	3	-8.125077849792056
4	4	-10.997424658628134
4	5	-7.734839195709242
4	6	-7.4014587906617635
4	7	-7.5327261651877
4	8	-8.83569366474089
4	9	-9.34523276315604
4	10	-5.6872984240779445
4	11	-7.757889688032517
4	12	-9.786168901579313
4	13	-6.734361224096226
4	14	-7.973804066508549
4	15	-9.20896745092847
4	16	-12.914325644319348
4	17	-8.995993659478632
4	18	-8.739944309620522
4	19	-12.939795768604966
4	20	-8.609307246664361
4	21	-10.955721957967917
4	22	-9.252619645694264
4	23	-5.691464334694103
4	24	-6.917797476810463
4	25	-7.962763600229312
4	26	-9.191499603177355
4	27	-8.4657673927539
4	28	-8.028315105365621
4	29	-6.552354933593772
4	30	-6.56117291632782
4	31	-7.906167480946152
4	32	-9.832277480561206
4	33	-8.586772251847794
4	34	-8.276205388937797
4	35	-11.440259002485202
4	36	-10.301396722395562
7	1	-5.025163525003634
7	2	-7.600641135120757
7	3	-7.866212852504717
7	4	-8.709698224766985
7	5	-6.751069646886467
7	6	-7.20878655939735
7	7	-6.670076477620654
7	8	-8.085798969754759
7	9	-7.651019428115365
7	10	-5.437728910974634
7	11	-11.45292075160754
7	12	-8.429492676025522
7	13	-6.447349741075886
7	14	-7.375675519133651
7	15	-5.26265222781101
7	16	-6.678106560467352
7	17	-5.864873472479392
7	18	-4.943646890070745
7	19	-7.111333423993045
7	20	-6.272016129689867
7	21	-6.623454592188285
7	22	-6.8291272484354515
7	23	-4.506907048204937
7	24	-5.344488338357117
7	25	-6.794423285875595
7	26	-8.962163816658421
7	27	-7.624745950968197
7	28	-7.824573211043553
7	29	-6.275703779057682
7	30	-6.54997014773264
7	31	-7.118483169090549
7	32	-10.166950855297664
7	33	-8.365412688299166
7	34	-7.795417516480981
7	35	-10.671598111587937
7	36	-10.275465292527581
8	1	-3.3268094038049227
8	2	-2.4552005155585164
8	3	-2.333674585250243
8	4	-2.628957731415163
8	5	-3.235974789581867
8	6	-2.576659154072381
8	7	-1.8831042897668198
8	8	-2.065434717315412
8	9	-1.9528254639371738
8	10	-2.5799720471553016
8	11	-2.2635631883890284
8	12	-2.1805207330955767
8	13	-3.3145492046611267
8	14	-1.8054984479287484
8	15	-3.158996947979709
8	16	-2.962267957738888
8	17	-3.1030717975515785
8	18	-3.4075864414730006
8	19	-3.4549854880635564
8	20	-3.492456387800061
8	21	-3.0134379888637355
8	22	-2.923888328191566
8	23	-2.780667571579521
8	24	-3.186799299104173
8	25	-4.295392309468639
8	26	-4.492776944937631
8	27	-4.385195640264463
8	28	-5.144872295838123
8	29	-4.794882103466083
8	30	-5.078065809575243
8	31	-4.792614202397694
8	32	-6.023869227868527
8	33	-5.666798753378558
8	34	-5.727191655294643
8	35	-6.827245107314788
8	36	-7.197865335870826
9	1	-2.5899609358234486
9	2	-2.3192895101225375
9	3	-2.422696231826599
9	4	-1.9142603104743898
9	5	-2.4717785393971425
9	6	-2.5980895436735523
9	7	-2.357930515191022
9	8	-3.031822191578314
9	9	-2.3870549893697657
9	10	-2.64879358347158
9	11	-2.90397192612798
9	12	-2.2025758467317544
9	13	-2.908272363485139
9	14	-2.085053699508464
9	15	-3.460958892816805
9	16	-2.5286184160093867
9	17	-2.4823020160055265
9	18	-3.3481272484630225
9	19	-3.247354079981541
9	20	-3.4031152507727924
9	21	-2.8413754837477327
9	22	-2.53848486147907
9	23	-2.652036389617699
9	24	-2.768233133491492
9	25	-4.62128527540532
9	26	-3.8092752390379445
9	27	-3.5103289680243748
9	28	-4.640340340073504
9	29	-4.271827645301698
9	30	-4.42821256329939
9	31	-4.232539976930411
9	32	-5.183282361574474
9	33	-5.075717134162513
9	34	-4.847469405389518
9	35	-6.315189674781571
9	36	-6.072659502044708
10	1	-2.4576876549513287
10	2	-2.1943833130628434
10	3	-2.111559552298104
10	4	-1.6707790757283263
10	5	-2.2306728639567055
10	6	-2.768241633441903
10	7	-3.36006400401979
10	8	-2.811152883607818
10	9	-2.3958386318822633
10	10	-2.4249660603257013
10	11	-2.691306833079139
10	12	-2.1327542772383907
10	13	-2.9725971323794624
10	14	-3.2578681105455516
10	15	-2.987534412727342
10	16	-2.345263674310793
10	17	-2.419487577225425
10	18	-2.9194170543400517
10	19	-2.9085480740712697
10	20	-2.7884353081453668
10	21	-2.9286595705268965
10	22	-2.739305348742377
10	23	-2.311709864337878
10	24	-2.8149711468036074
10	25	-4.704005029773647
10	26	-3.3405300483538483
10	27	-3.3840593779830073
10	28	-4.131494073994219
10	29	-3.863231596059343
10	30	-4.195158568569049
10	31	-3.7133318939253073
10	32	-4.668160977026176
10	33	-4.303786212269461
10	34	-4.397454235000705
10	35	-5.662819928900085
10	36	-5.416633124988287
11	1	-2.342987610973225
11	2	-2.3434338349048645
11	3	-2.5555921219649846
11	4	-1.6550480398053224
11	5	-1.8815347442783548
11	6	-2.2543128469156954
11	7	-2.4787514048380714
11	8	-2.953077484110324
11	9	-2.895938327204334
11	10	-2.1382523587607807
11	11	-2.205740561722642
11	12	-1.9499835166139974
11	13	-2.8222538680970857
11	14	-2.4246156208126948
11	15	-2.60013918965101
11	16	-2.5088744324800842
11	17	-2.2077554851548697
11	18	-2.777463564275436
11	19	-2.7300100035437183
11	20	-2.68740156288014
11	21	-2.4566080946526085
11	22	-2.282859341143485
11	23	-2.267655709439964
11	24	-2.4463956948773955
11	25	-4.176519774299727
11	26	-3.0771789228770885
11	27	-3.0848192046539893
11	28	-3.612795938227748
11	29	-3.5752689880440798
11	30	-4.324452513643713
11	31	-3.8815555404799937
11	32	-3.830538372441705
11	33	-4.0428114040647145
11	34	-4.0477870411968695
11	35	-4.857670336649672
11	36	-5.026689618337226
12	1	-2.3374844518731095
12	2	-1.8111804477303082
12	3	-1.8495615316029657
12	4	-1.6122232433549541
12	5	-1.514877541965454
12	6	-2.4601748172320583
12	7	-1.5699566325560141
12	8	-2.1406380077769924
12	9	-2.6664073724071713
12	10	-2.0629172555900297
12	11	-1.8258738552608804
12	12	-1.689786714136531
12	13	-2.374766277665614
12	14	-1.776638141661465
12	15	-2.834200639628669
12	16	-2.3337134349445563
12	17	-2.0347325982756317
12	18	-2.4362940962946666
12	19	-2.5812741347650174
12	20	-2.32818032012856
12	21	-2.4454274365256055
12	22	-2.619980796162743
12	23	-1.9713687767836419
12	24	-2.613408651903292
12	25	-3.4253267517274395
12	26	-2.6216239059281463
12	27	-2.8341336561753607
12	28	-3.581803700996069
12	29	-3.188854072721867
12	30	-3.387812051252247
12	31	-3.2395078243467736
12	32	-3.332188250721657
12	33	-3.2695140127315483
12	34	-3.251542784070459
12	35	-3.783271218893823
12	36	-4.359317522180081
13	1	-1.3234678568101472
13	2	-1.718095782347092
13	3	-1.2348230181713689
13	4	-1.2471780171523932
13	5	-1.539796928900202
13	6	-1.687648694825313
13	7	-2.3620403360857196
13	8	-1.8067628783276533
13	9	-2.4582019958375696
13	10	-1.7894040804517815
13	11	-2.108319816640858
13	12	-1.455068942190593
13	13	-2.1264410265268197
13	14	-1.8426510257534474
13	15	-2.280661714667964
13	16	-2.07393309697531
13	17	-1.776732467200624
13	18	-2.2633018719349636
13	19	-2.501359139856092
13	20	-2.113105896774151
13	21	-2.804986061271414
13	22	-2.193260749830486
13	23	-1.8113579974932534
13	24	-2.3385061957232764
13	25	-3.2122177715430666
13	26	-2.3097731947887823
13	27	-2.576514201370429
13	28	-3.1748853393625396
13	29	-2.892367182494098
13	30	-2.9920648590286305
13	31	-2.8959258578002363
13	32	-3.316647449034498
13	33	-2.8357105378335703
13	34	-3.1433883132283356
13	35	-4.0815137907991215
13	36	-4.001837434975041
14	1	-1.7749502726819273
14	2	-1.0907284465336389
14	3	-1.5442827608366727
14	4	-1.0921456339179372
14	5	-0.9896697204896157
14	6	-1.8363157064169329
14	7	-1.9393328535047942
14	8	-2.1321485010165304
14	9	-2.5180235801252167
14	10	-1.3243338224858334
14	11	-1.3614073273099478
14	12	-1.2724915232704461
14	13	-1.7663281477232502
14	14	-2.526891884836836
14	15	-1.7489552815847418
14	16	-1.942342427987093
14	17	-1.5333024184541486
14	18	-1.6525497113528118
14	19	-1.7977760412843367
14	20	-1.6417012227922498
14	21	-1.9016116402699055
14	22	-2.007830699475967
14	23	-1.5083346201671333
14	24	-1.8381583259105128
14	25	-2.861211054776362
14	26	-2.135366222104601
14	27	-2.330528041481962
14	28	-4.317016112028387
14	29	-2.337176816257937
14	30	-2.492627545687391
14	31	-2.2872026308112225
14	32	-2.3757919472995326
14	33	-2.19077539154474
14	34	-2.5139162400217714
14	35	-3.01942986552978
14	36	-2.971006467904104
15	1	-1.5324324388636015
15	2	-0.9528618037861207
15	3	-1.0287105110825223
15	4	-0.8407856373333829
15	5	-0.953449120047857
15	6	-1.3378573354390042
15	7	-2.060078393832356
15	8	-1.3047658263092146
15	9	-2.0839910688190315
15	10	-1.3122743317514605
15	11	-1.2770634303987833
15	12	-1.026046286664993
15	13	-1.6938231976995937
15	14	-2.622457886591036
15	15	-1.6187446538027643
15	16	-1.5570905372947763
15	17	-1.2528554810937393
15	18	-1.47762092173744
15	19	-1.3846034958242939
15	20	-1.360197004465924
15	21	-1.9815166323123847
15	22	-1.762742676754506
15	23	-1.3732595784270671
15	24	-1.6587676274456982
15	25	-1.89762812186639
15	26	-1.6684203271784384
15	27	-2.000000690813195
15	28	-2.2431002872283656
15	29	-1.9377969825341808
15	30	-2.0014481335471572
15	31	-2.1400607213762086
15	32	-2.2959536335556834
15	33	-1.8516089761146048
15	34	-2.0257994785291515
15	35	-2.6691177180208214
15	36	-2.4814985885266836
16	1	-1.1759822199179055
16	2	-1.0589200825210838
16	3	-1.0003854498238312
16	4	-0.5581057685446353
16	5	-0.7171461536597974
16	6	-0.6610560385987228
16	7	-1.6222928680223347
16	8	-1.2972552466208853
16	9	-1.192565580595435
16	10	-0.9848869446826569
16	11	-1.0005942780147095
16	12	-0.7273265417341577
16	13	-1.0621189724879017
16	14	-2.2562942152786363
16	15	-1.2538585495290064
16	16	-1.6516832339663097
16	17	-1.0415015325985308
16	18	-1.208481423204141
16	19	-1.1341492325841713
16	20	-1.032469236010222
16	21	-1.7289244165985522
16	22	-1.403623164265042
16	23	-1.0678333487573397
16	24	-1.374928441100515
16	25	-1.5372291120466528
16	26	-1.3393970913647308
16	27	-1.4269187588259555
16	28	-2.0395773774244006
16	29	-1.4224998847558012
16	30	-1.5395651176574248
16	31	-1.6658313506209126
16	32	-1.977100312076395
16	33	-1.452503978622893
16	34	-1.6888642082528804
16	35	-2.1363633272795823
16	36	-1.879117120738492
17	1	-0.9042699163225052
17	2	-0.47810460298988033
17	3	-1.1343121107555802
17	4	-0.735841129602548
17	5	-0.6491483953594255
17	6	-1.2366520215271422
17	7	-1.9845875735656477
17	8	-1.0773351469931214
17	9	-1.2722007104993907
17	10	-0.8256022798588702
17	11	-1.073817979247309
17	12	-0.5692015522487281
17	13	-0.9368737438023018
17	14	-2.281379983109076
17	15	-0.9831027519719342
17	16	-0.6811125412321175
17	17	-0.7627287837652901
17	18	-1.0074579939673924
17	19	-0.7656317298613331
17	20	-0.8101555975870056
17	21	-1.099958888579117
17	22	-1.0869203826610558
17	23	-0.6967443153630626
17	24	-1.0949659822568805
17	25	-1.0746482839061073
17	26	-0.9735236608072952
17	27	-1.354958012897022
17	28	-1.550601639505068
17	29	-1.110773408324519
17	30	-1.1399803173415037
17	31	-1.26807662767537
17	32	-1.4964385759531922
17	33	-1.0744016353628112
17	34	-1.3371561727797356
17	35	-1.657832782491738
17	36	-1.4649008552768237
18	1	-0.3749945265272885
18	2	-0.543072484311601
18	3	-1.0755377204241299
18	4	-0.38388751974802915
18	5	-0.5785322484082727
18	6	-0.7231472224628988
18	7	-0.8180020523938865
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109	3	-0.5552346326098956
109	4	0.17978112959298898
109	5	0.022564923485134614
109	6	0.2394374007489688
109	7	-0.005490304515141986
109	8	0.13317618669340447
109	9	-0.5182830977903947
109	10	-0.009511854944740605
109	11	-0.179078285667309
109	12	-0.03618092190069433
109	13	-0.04243693402779769
109	14	-1.384319803695604
109	15	-0.06088816611913472
109	16	-0.16405300520047916
109	17	-0.14103042434368973
109	18	-0.09886436110714669
109	19	-0.08570574430500244
109	20	-0.16725369541479296
109	21	-0.42961124495162295
109	22	0.006473995184286707
109	23	-0.011695221483002172
109	24	-0.10776937045064727
109	25	-6.358388196829878E-4
109	26	-0.13687110493911092
109	27	-0.0994660663803657
109	28	-0.08408310728376733
109	29	-0.25312107583717136
109	30	-0.1497402166146813
109	31	-0.07481607643255472
109	32	-0.15486858878652754
109	33	-0.19209344189926014
109	34	-0.21692959518527033
109	35	-0.40573057803692336
109	36	-0.45771130598724596
110	1	-0.08298996259514269
110	2	-0.17416603264526742
110	3	-0.263242858714662
110	4	0.05048862808219923
110	5	-0.07288869267798503
110	6	0.31914217850071935
110	7	-0.8952665034887275
110	8	0.2708554273569348
110	9	-0.7000375139907291
110	10	-0.17291795792420572
110	11	0.04859309062605632
110	12	-0.2979466491164331
110	13	-0.20411305355252551
110	14	-0.7213571355284358
110	15	-0.23267975621460124
110	16	0.026122598498602656
110	17	-0.27353812921401044
110	18	-0.22674203414441507
110	19	-0.6564345224104997
110	20	-0.35389443344090665
110	21	-0.08532872327151608
110	22	-0.3889049082872589
110	23	-0.3069991988388736
110	24	-0.25597671446741543
110	25	-0.5154146280646401
110	26	-0.33763353058792234
110	27	-0.47974960600870986
110	28	-0.29265359997012297
110	29	-0.6674624531443811
110	30	-0.4257603126748677
110	31	-0.33557170278994874
110	32	-0.48100316549943756
110	33	-0.519803169426055
110	34	-0.40982308554928437
110	35	-0.501973104470764
110	36	-0.6006339454999576
111	1	-0.25149509298696415
111	2	-0.4055578166801155
111	3	-0.6387597514516443
111	4	-0.027152974530579854
111	5	-0.22516074397208996
111	6	-0.039970308531003695
111	7	-0.7013688223175265
111	8	-0.780096805663824
111	9	-0.4198109829271104
111	10	-0.34617108608642866
111	11	-0.3150778729217052
111	12	-0.4606215178284714
111	13	-0.5332085906933131
111	14	-1.439702625276162
111	15	-0.4582911994947665
111	16	-0.3045608208348938
111	17	-0.49716352394077035
111	18	-0.478051257061459
111	19	-0.8891685047093247
111	20	-0.5060770144346733
111	21	-0.5895568163930065
111	22	-0.893908262998778
111	23	-0.5923930880134449
111	24	-0.5260721885960677
111	25	-0.7439042561521205
111	26	-0.6572871843378465
111	27	-0.8123603392441581
111	28	-0.6036209643973152
111	29	-0.8306521864496332
111	30	-0.7120233954927357
111	31	-0.6884027304366569
111	32	-0.863642260075153
111	33	-0.6539739858071982
111	34	-0.7810419053015423
111	35	-0.9585859153357035
111	36	-1.2618046957454752
112	1	-0.47750838478933133
112	2	-0.5542802345163054
112	3	-1.1329335732189776
112	4	-0.2837394252997356
112	5	-0.6476978226282668
112	6	-0.7723186971638185
112	7	-1.6117458801600222
112	8	-0.9801023458819316
112	9	-1.8865914713280674
112	10	-0.9053941258453904
112	11	-1.0502317451491479
112	12	-0.7398779304409473
112	13	-0.9489829205412832
112	14	-2.9405019399408303
112	15	-0.9912722987898519
112	16	-1.301011967480143
112	17	-0.9842049975037455
112	18	-0.8896273645769881
112	19	-1.0423027331310877
112	20	-0.9447351526266914
112	21	-2.2915927940905547
112	22	-1.664466398007761
112	23	-0.8427899032771009
112	24	-0.9654415662422764
112	25	-1.4349489562767843
112	26	-1.1552364838368518
112	27	-1.3646570464789463
112	28	-1.5973456202785172
112	29	-1.8034807886521307
112	30	-1.4339936240230955
112	31	-1.1580623029990846
112	32	-1.7529453195351792
112	33	-1.3639037265204834
112	34	-1.3163377814296382
112	35	-1.7799361243249265
112	36	-1.7929486727048953
113	1	-0.7761348750913797
113	2	-1.6244075319559812
113	3	-1.411911318935097
113	4	-0.5205413965044556
113	5	-0.9217866804724508
113	6	-1.4504170647189056
113	7	-1.371352019327098
113	8	-1.0113146788140597
113	9	-1.5427996086143736
113	10	-1.2382291165431738
113	11	-1.1882971386565444
113	12	-0.8413892838684731
113	13	-1.122096268260293
113	14	-2.390346265389887
113	15	-1.2934835704355336
113	16	-1.1100492323628544
113	17	-1.1338886638130035
113	18	-1.169997080832701
113	19	-1.3645536724071121
113	20	-1.2462056287395953
113	21	-1.242670948468671
113	22	-3.272507456514184
113	23	-1.0919745841713535
113	24	-1.2450372662966203
113	25	-1.8844497505531521
113	26	-1.4577759416069256
113	27	-1.5208521608889
113	28	-1.5269233247762202
113	29	-2.229402780653075
113	30	-1.7148351438064324
113	31	-1.3959548160507822
113	32	-2.139760526836433
113	33	-1.6808292719775508
113	34	-1.6475032056379106
113	35	-2.186967743760974
113	36	-2.3337888524334076
114	1	-1.2152351593220772
114	2	-1.6617369117770906
114	3	-2.0044605761742815
114	4	-1.139178482812767
114	5	-1.1565048394245991
114	6	-1.7977495588878654
114	7	-1.868302448423286
114	8	-1.5573524887808716
114	9	-1.5554639294858603
114	10	-1.6291462771201424
114	11	-1.3461605696718877
114	12	-1.1670672491513017
114	13	-1.5419889793484103
114	14	-1.8939888073815538
114	15	-1.7049343362380611
114	16	-1.5368469506384983
114	17	-1.4053090777313884
114	18	-1.551486816863843
114	19	-2.080912676440157
114	20	-1.5258255853914378
114	21	-2.3267702144042417
114	22	-2.133138364669911
114	23	-1.4886139687048612
114	24	-1.5809162073588894
114	25	-2.393956278791144
114	26	-1.7678678780474755
114	27	-1.9795423620653594
114	28	-2.2423982516745227
114	29	-2.634843505471082
114	30	-2.1964018094325084
114	31	-1.9007164420822704
114	32	-2.7986808664267073
114	33	-2.2128089974986977
114	34	-2.2619218371681953
114	35	-2.9112702673489963
114	36	-2.919147924105026
115	1	-1.4172574419146358
115	2	-1.8981279779568325
115	3	-1.5747810090592935
115	4	-1.1573419132334806
115	5	-1.2818214125667282
115	6	-2.081245075648056
115	7	-1.3664015250540231
115	8	-1.6781012046740997
115	9	-2.080911511208491
115	10	-1.7202722086542437
115	11	-1.6595085997184995
115	12	-1.4179761443741299
115	13	-1.9247466914115918
115	14	-2.0364188866547295
115	15	-1.9359815141722052
115	16	-2.191133120882853
115	17	-1.7927259448077848
115	18	-1.923882974935161
115	19	-2.477146829226591
115	20	-1.9586486161803123
115	21	-1.9832074490469225
115	22	-2.947615740638554
115	23	-1.5976995807710068
115	24	-1.8209695757268136
115	25	-2.9531491471739173
115	26	-2.3299139871297427
115	27	-2.4907077817381733
115	28	-2.529286259750293
115	29	-3.2804125948686647
115	30	-2.8252971807100806
115	31	-2.407280602885095
115	32	-3.4325040902027197
115	33	-2.9087165566566853
115	34	-2.940838362805792
115	35	-3.6950567493418127
115	36	-3.961749093904025
116	1	-1.7133739613088323
116	2	-1.7640542271193027
116	3	-1.4843336066127435
116	4	-1.4831837016952036
116	5	-1.9502147759569866
116	6	-5.866978742261399
116	7	-1.3362176794878664
116	8	-1.820910872221603
116	9	-1.5557747696947644
116	10	-3.3926332634598637
116	11	-1.9312101540736677
116	12	-2.081342958182887
116	13	-2.389771720320887
116	14	-2.8562136525161392
116	15	-3.6772532866082273
116	16	-2.8441866016775776
116	17	-1.9322063308844335
116	18	-2.4598013434136305
116	19	-3.138240810965712
116	20	-2.3569946990530894
116	21	-3.4406093524830155
116	22	-3.0186638372960033
116	23	-3.2594058056098945
116	24	-2.2335548228626507
116	25	-5.879957613227528
116	26	-2.72652774151868
116	27	-3.234385042792738
116	28	-3.061440252082356
116	29	-4.770469087239913
116	30	-3.46584466903564
116	31	-2.811750701596864
116	32	-6.184980758551841
116	33	-3.3342958401461473
116	34	-3.6442889587899714
116	35	-5.643214685379419
116	36	-4.661347546490173
117	1	-1.7596754247142457
117	2	-1.8858663238787798
117	3	-1.6931927183981514
117	4	-1.4419345090591875
117	5	-1.6996967313508975
117	6	-2.3145942577278547
117	7	-2.074626654069938
117	8	-1.99402246762115
117	9	-1.0961394219471998
117	10	-2.1652872402283947
117	11	-1.8990812611864456
117	12	-1.8839950137591899
117	13	-2.4574741184415174
117	14	-1.387645494789652
117	15	-2.1968636353121056
117	16	-2.2613316996362087
117	17	-2.3301853088279003
117	18	-2.584473178983309
117	19	-3.1468974377135908
117	20	-3.006091611810625
117	21	-2.0287959711574857
117	22	-2.563566930837453
117	23	-2.18116527215998
117	24	-2.447608073369757
117	25	-3.4924196530162988
117	26	-3.485907857824344
117	27	-3.2544126598927434
117	28	-3.783011387977858
117	29	-4.199097343455101
117	30	-3.782108382221672
117	31	-3.536038136660302
117	32	-5.071450116361461
117	33	-4.313648191356108
117	34	-4.307195601843372
117	35	-5.32646119813116
117	36	-5.681908226397134
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	26121.0
3	1597.0
4	742.0
5	509.0
6	565.0
7	492.0
8	680.0
9	1243.0
10	1590.0
11	1428.0
12	1931.0
13	2488.0
14	3066.0
15	3155.0
16	3971.0
17	5548.0
18	8198.0
19	10733.0
20	13506.0
21	20165.0
22	28822.0
23	40076.0
24	55818.0
25	86421.0
26	135612.0
27	172505.0
28	216785.0
29	270024.0
30	383432.0
31	516362.0
32	660950.0
33	878534.0
34	1249755.0
35	1840166.0
36	2888437.0
37	4842813.0
38	7325655.0
39	8047458.0
40	1360.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	81.49791502676831	4.6049071870285525	4.581498276306654	9.315679509896487
2	9.306375707749105	44.700922019718966	3.6906369697405057	42.30206530279142
3	46.374936055294214	44.7778226952896	4.592276992229285	4.254964257186901
4	42.60186958337558	3.864777577369976	48.03998099786232	5.49337184139212
5	8.588471824632219	4.114259859597132	81.62168876064418	5.67557955512648
6	42.98107488546086	8.84179455378772	3.2040974077237	44.97303315302772
7	6.45467674227406	82.39212512233317	3.499466430071279	7.653731705321494
8	45.75169669095136	5.091903189796132	3.6072538574115036	45.549146261841
9	5.516863872396765	43.95919917611226	4.618411559518558	45.90552539197242
10	8.401847260800945	5.204544091613293	43.86388566409768	42.529722983488085
11	47.36433843742984	4.021019963579597	4.68397806868587	43.93066353030469
12	49.06385840435237	5.403713479203289	40.85837255254853	4.674055563895818
13	42.226368435157255	11.271033105563912	5.168781860523468	41.33381659875537
14	8.574292974024967	45.04823652435633	4.019357403010285	42.35811309860843
15	42.45515256635488	8.900521448249382	8.204767451937258	40.43955853345848
16	42.907254126278985	41.79585598071577	11.273141592451827	4.02374830055342
17	41.282063297246104	5.566059858300825	42.20971528932265	10.942161555130424
18	40.468728039991284	8.383535282356572	9.448900235200359	41.69883644245178
19	11.404929063179495	5.2843565532626595	78.17451915052966	5.136195233028182
20	42.14243722013666	12.859283697076753	5.585510021607352	39.41276906117924
21	4.61941047775701	82.04162250186799	7.126121084448562	6.212845935926429
22	4.445334568211986	44.41704826353257	9.105952695972446	42.031664472283
23	7.09551104794318	4.99655294343738	48.66921161821263	39.238724390406816
24	38.97065116436982	6.828628871638467	41.93193251068702	12.268787453304698
25	6.597653485043202	12.081473911157097	5.930088471390342	75.39078413240937
26	15.624892325899403	68.37577419884335	5.45571410673667	10.543619368520583
27	68.92186087431342	17.037379363043996	7.4893623400989515	6.55139742254363
28	12.75955248272474	64.1811405124451	15.260144645786983	7.799162359043173
29	10.981121611188204	10.49297800877925	63.165064100678556	15.360836279353993
30	15.79931087902558	9.633359645942232	11.398888416094119	63.16844105893807
31	74.72917680660764	8.774866098994687	6.33242933718225	10.163527757215434
32	65.6328475724344	20.606577310221944	6.318058661595202	7.4425164557484615
33	11.397479474784411	71.82045346326403	7.001426810695718	9.780640251255834
34	15.49709430532249	63.95901235962308	9.434253447571104	11.109639887483327
35	68.34164347220027	14.43389483792481	9.24819069043251	7.976270999442405
36	17.333865089916962	63.442643621759856	7.177930631649976	12.045560656673215
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	461.0
1	296.0
2	131.0
3	131.0
4	148.0
5	165.0
6	165.0
7	289.0
8	413.0
9	413.0
10	748.0
11	1083.0
12	1083.0
13	2043.0
14	3003.0
15	5090.5
16	7178.0
17	7178.0
18	10848.0
19	14518.0
20	14518.0
21	20818.5
22	27119.0
23	27119.0
24	36874.5
25	46630.0
26	60361.5
27	74093.0
28	74093.0
29	93722.0
30	113351.0
31	113351.0
32	136630.0
33	159909.0
34	159909.0
35	184122.5
36	208336.0
37	208336.0
38	250137.5
39	291939.0
40	292736.0
41	293533.0
42	293533.0
43	357189.0
44	420845.0
45	420845.0
46	496408.5
47	571972.0
48	571972.0
49	759498.0
50	947024.0
51	1919329.5
52	2891635.0
53	2891635.0
54	1.13455925E7
55	1.979955E7
56	1.979955E7
57	1.08639225E7
58	1928295.0
59	1928295.0
60	1460183.0
61	992071.0
62	992071.0
63	749779.0
64	507487.0
65	366470.5
66	225454.0
67	225454.0
68	164416.0
69	103378.0
70	103378.0
71	78888.0
72	54398.0
73	54398.0
74	42306.5
75	30215.0
76	23497.5
77	16780.0
78	16780.0
79	12989.5
80	9199.0
81	9199.0
82	6955.5
83	4712.0
84	4712.0
85	3489.0
86	2266.0
87	2266.0
88	1614.0
89	962.0
90	643.5
91	325.0
92	325.0
93	224.0
94	123.0
95	123.0
96	85.5
97	48.0
98	48.0
99	80.0
100	112.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.4429973155477348
2	0.0
3	0.0011933289349357736
4	0.0016168766695890341
5	1.1092916859966345E-4
6	4.975005137197027E-4
7	1.1765214851479457E-4
8	2.4202727694472027E-4
9	0.0
10	5.714532927861451E-5
11	0.012931651866754707
12	3.8657134512003937E-4
13	0.0036472166039586314
14	0.0010118084772272333
15	0.0013815723725594448
16	1.9496641753880245E-4
17	1.9160492758123687E-4
18	1.1429065855722902E-4
19	1.9496641753880245E-4
20	1.411825782177535E-4
21	9.580246379061844E-4
22	1.3782108826018792E-4
23	4.8405455388944054E-4
24	1.0084469872696677E-5
25	0.0
26	0.0012773661838749125
27	5.344769032529239E-4
28	0.006181780031963063
29	0.005122910695329912
30	0.004205223936914515
31	0.0014454406817531905
32	0.014266163379908235
33	0.0018420964967459264
34	7.832271601127753E-4
35	0.004423720784156275
36	0.002574901307495218
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
36	2.9748713E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.810141423955994
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.54197005259125	22.21548940384421
2	5.498121752768136	2.728183565046125
3	2.0419982599368103	1.5198679684951293
4	1.011614354518561	1.0039318080843782
5	0.5363622683468086	0.6653611866079077
6	0.34621652267448794	0.5153808534518588
7	0.2147852639596032	0.3730196942233632
8	0.15353220980494808	0.3047324670714596
9	0.11362920392088716	0.25372409572518706
>10	0.47198249867120595	2.0624257228708336
>50	0.03444148774826038	0.5869159216314169
>100	0.026084286184419193	1.3168318281417166
>500	0.003340930218574625	0.574591995593945
>1k	0.004285852157288456	2.389760412536747
>5k	7.06047124552161E-4	1.2451759206112873
>10k+	9.290093744107383E-4	62.244607156064546
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATTCAGATCTCCCGGGTCACCGCAGATCGGAAGA	8568281	28.802190535099786	No Hit
GCGGTGACCCGGGAGATCTGAATTCAGATCGGAAGA	6876064	23.11382008357807	No Hit
CGGTGACCCGGGAGATCTGAATTCAGATCGGAAGAG	732412	2.4619955828005065	No Hit
GAATTCAGATCTCCCGGGTCACCGAGATCGGAAGAG	642826	2.160853143462038	No Hit
GAATTCAGATCGAATTCAGATCTCCCGGGTCACCGC	549244	1.846278190253138	No Hit
GCGGTGACCCGGGAGATCTGAATTCGATCTGAATTC	478126	1.6072157474509907	No Hit
TGACCCGGGAGATCTGAATTCAGATCGGAAGAGCGG	276734	0.9302385619169474	No Hit
GAATTCAGATCTCCCGGGTCAAGATCGGAAGAGCGG	235633	0.7920779631710454	No Hit
AATTCAGATCTCCCGGGTCACCGCAGATCGGAAGAG	122039	0.4102328729313433	No Hit
CCCGGGAGATCTGAATTCAGATCGGAAGAGCGGTTC	78112	0.26257270356536094	No Hit
GAATTCAGATCTCCCGGGAGATCGGAAGAGCGGTTC	76858	0.25835739515857375	No Hit
GCGGTGACCCGGGAGATCTGAATTAGATCGGAAGAG	71196	0.23932463901883755	No Hit
ACCCGGGAGATCTGAATTCAGATCGGAAGAGCGGTT	64021	0.21520594857330466	No Hit
GAATTCAGATCTCCCGGGTAGATCGGAAGAGCGGTT	56946	0.19142340712352834	No Hit
GAATTCAGATCGAATTCAGATCTCCCGGGTCACCGA	50629	0.1701888750615867	No Hit
CGGTGACCCGGGAGATCTGAATTCGATCTGAATTCA	50478	0.16968129007799432	No Hit
GAATTCAGATCTCCCGGGTCAGATCGGAAGAGCGGT	49512	0.16643409077898597	No Hit
GAATTCAGATCGAATTCAGATCGAATTCAGATCTCC	42802	0.1438784931637211	No Hit
GACCCGGGAGATCTGAATTCAGATCGGAAGAGCGGT	42572	0.14310535047348097	No Hit
GAATTCAGATCTCCCGGATCACCGCAGATCGGAAGA	41098	0.13815051427602934	No Hit
GTGACCCGGGAGATCTGAATTCAGATCGGAAGAGCG	38997	0.13108802387518412	No Hit
NAATTCAGATCTCCCGGGTCACCGCAGATCGGAAGA	37691	0.12669791799060348	No Hit
CCGGGAGATCTGAATTCAGATCGGAAGAGCGGTTCA	34649	0.11647226553968906	No Hit
GAATTCAGATCTCCCGAGTCACCGCAGATCGGAAGA	33894	0.11393434062172707	No Hit
GCGGTGACCCGGGAGATCTGAATTCAAGATCGGAAG	33491	0.11257966016882816	No Hit
GAATTCAGATCTCCCGGGTCACCGCAAGATCGGAAG	32809	0.11028712401776843	No Hit
GCGGTGACCGGGAGATCTGAATTCAGATCGGAAGAG	32645	0.10973583966472768	No Hit
GAATTCAGATCTCCCGGAGATCGGAAGAGCGGTTCA	32523	0.10932573788990467	No Hit
GAATTCAGATCTCCCGGTCACCGCAGATCGGAAGAG	31677	0.10648191738580422	No Hit
GAATTCAGATCTCCCGGGTCACAGATCGGAAGAGCG	31543	0.10603147773149044	No Hit
GAATTCAGATCTCCCAGGTCACCGCAGATCGGAAGA	30959	0.10406836759627215	No Hit
NCGGTGACCCGGGAGATCTGAATTCAGATCGGAAGA	30832	0.10364145837166133	No Hit
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTT	30505	0.10254225115553738	Illumina Paired End PCR Primer 2 (96% over 25bp)
ATTCAGATCTCCCGGGTCACCGCAGATCGGAAGAGC	30363	0.10206491958156308	No Hit
GAATTCAGATCTCCCGGGTCACCACAGATCGGAAGA	30314	0.10190020657364236	No Hit
GGTGACCCGGGAGATCTGAATTCAGATCGGAAGAGC	30197	0.1015069122486072	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.8824343762367132E-4	0.0	0.0	3.361489957565559E-6
2	4.605241241864816E-4	0.0	0.0	3.361489957565559E-6
3	9.176867584153977E-4	0.0	0.0	3.361489957565559E-6
4	0.0014689711114561493	0.0	0.0	3.361489957565559E-6
5	0.001905964805939672	3.361489957565559E-6	0.0	3.361489957565559E-6
6	0.0026051547171133086	3.361489957565559E-6	0.0	6.722979915131118E-6
7	0.03497294151851208	3.361489957565559E-6	3.361489957565559E-6	6.722979915131118E-6
8	0.03527547561469298	3.361489957565559E-6	6.722979915131118E-6	1.3445959830262236E-5
9	0.03552422587155283	3.361489957565559E-6	6.722979915131118E-6	1.3445959830262236E-5
10	0.035860374867309384	3.361489957565559E-6	6.722979915131118E-6	1.3445959830262236E-5
11	0.03613937853378733	3.361489957565559E-6	6.722979915131118E-6	1.3445959830262236E-5
12	0.03647888901950145	3.361489957565559E-6	6.722979915131118E-6	1.3445959830262236E-5
13	0.03730917703902014	3.361489957565559E-6	6.722979915131118E-6	1.3445959830262236E-5
14	0.04948113217536503	3.361489957565559E-6	6.722979915131118E-6	1.6807449787827796E-5
15	0.058049570077199644	3.361489957565559E-6	6.722979915131118E-6	1.6807449787827796E-5
16	0.06097070485032411	3.361489957565559E-6	6.722979915131118E-6	1.6807449787827796E-5
17	0.08378513719232156	3.361489957565559E-6	6.722979915131118E-6	1.6807449787827796E-5
18	0.3516891638303815	3.361489957565559E-6	6.722979915131118E-6	1.6807449787827796E-5
19	0.9344370628739469	3.361489957565559E-6	6.722979915131118E-6	1.6807449787827796E-5
20	1.5304090634105751	3.361489957565559E-6	6.722979915131118E-6	1.6807449787827796E-5
21	1.9758098442779692	3.361489957565559E-6	6.722979915131118E-6	1.6807449787827796E-5
22	3.8101782756114524	3.361489957565559E-6	6.722979915131118E-6	1.6807449787827796E-5
23	4.175659632737726	6.722979915131118E-6	6.722979915131118E-6	2.0168939745393357E-5
24	4.850519079598502	6.722979915131118E-6	6.722979915131118E-6	2.0168939745393357E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGGTGA	1120150	0.0	30.062952	1
ACCGCAG	952435	0.0	28.687174	21
CCGCAGA	951600	0.0	28.671051	22
CGCAGAT	952015	0.0	28.634472	23
CACCGCA	962575	0.0	28.584448	20
GCAGATC	952125	0.0	28.578127	24
ACCGAGA	73895	0.0	28.248251	21
CACCGAG	74975	0.0	28.169428	20
TATGCCG	1630	0.0	27.87805	3
CGGTGAC	1236130	0.0	27.177448	2
ACCGCAA	5925	0.0	27.032207	21
TCACCGC	1020990	0.0	26.999905	19
ACCGCGA	1840	0.0	26.97794	21
GGTGACC	1246140	0.0	26.957453	3
GGGTCAC	1113690	0.0	26.952513	16
GTCACCG	1102430	0.0	26.937143	18
CGGGTCA	1150060	0.0	26.908031	15
GGTCACC	1111715	0.0	26.880516	17
CCGGGTC	1151705	0.0	26.875895	14
CCGCAAG	4010	0.0	26.853298	22
>>END_MODULE
