FastQCFastQC Report
Sun 14 Aug 2016
V503_input.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameV503_input.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29748713
Sequences flagged as poor quality0
Sequence length36
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATTCAGATCTCCCGGGTCACCGCAGATCGGAAGA856828128.802190535099786No Hit
GCGGTGACCCGGGAGATCTGAATTCAGATCGGAAGA687606423.11382008357807No Hit
CGGTGACCCGGGAGATCTGAATTCAGATCGGAAGAG7324122.4619955828005065No Hit
GAATTCAGATCTCCCGGGTCACCGAGATCGGAAGAG6428262.160853143462038No Hit
GAATTCAGATCGAATTCAGATCTCCCGGGTCACCGC5492441.846278190253138No Hit
GCGGTGACCCGGGAGATCTGAATTCGATCTGAATTC4781261.6072157474509907No Hit
TGACCCGGGAGATCTGAATTCAGATCGGAAGAGCGG2767340.9302385619169474No Hit
GAATTCAGATCTCCCGGGTCAAGATCGGAAGAGCGG2356330.7920779631710454No Hit
AATTCAGATCTCCCGGGTCACCGCAGATCGGAAGAG1220390.4102328729313433No Hit
CCCGGGAGATCTGAATTCAGATCGGAAGAGCGGTTC781120.26257270356536094No Hit
GAATTCAGATCTCCCGGGAGATCGGAAGAGCGGTTC768580.25835739515857375No Hit
GCGGTGACCCGGGAGATCTGAATTAGATCGGAAGAG711960.23932463901883755No Hit
ACCCGGGAGATCTGAATTCAGATCGGAAGAGCGGTT640210.21520594857330466No Hit
GAATTCAGATCTCCCGGGTAGATCGGAAGAGCGGTT569460.19142340712352834No Hit
GAATTCAGATCGAATTCAGATCTCCCGGGTCACCGA506290.1701888750615867No Hit
CGGTGACCCGGGAGATCTGAATTCGATCTGAATTCA504780.16968129007799432No Hit
GAATTCAGATCTCCCGGGTCAGATCGGAAGAGCGGT495120.16643409077898597No Hit
GAATTCAGATCGAATTCAGATCGAATTCAGATCTCC428020.1438784931637211No Hit
GACCCGGGAGATCTGAATTCAGATCGGAAGAGCGGT425720.14310535047348097No Hit
GAATTCAGATCTCCCGGATCACCGCAGATCGGAAGA410980.13815051427602934No Hit
GTGACCCGGGAGATCTGAATTCAGATCGGAAGAGCG389970.13108802387518412No Hit
NAATTCAGATCTCCCGGGTCACCGCAGATCGGAAGA376910.12669791799060348No Hit
CCGGGAGATCTGAATTCAGATCGGAAGAGCGGTTCA346490.11647226553968906No Hit
GAATTCAGATCTCCCGAGTCACCGCAGATCGGAAGA338940.11393434062172707No Hit
GCGGTGACCCGGGAGATCTGAATTCAAGATCGGAAG334910.11257966016882816No Hit
GAATTCAGATCTCCCGGGTCACCGCAAGATCGGAAG328090.11028712401776843No Hit
GCGGTGACCGGGAGATCTGAATTCAGATCGGAAGAG326450.10973583966472768No Hit
GAATTCAGATCTCCCGGAGATCGGAAGAGCGGTTCA325230.10932573788990467No Hit
GAATTCAGATCTCCCGGTCACCGCAGATCGGAAGAG316770.10648191738580422No Hit
GAATTCAGATCTCCCGGGTCACAGATCGGAAGAGCG315430.10603147773149044No Hit
GAATTCAGATCTCCCAGGTCACCGCAGATCGGAAGA309590.10406836759627215No Hit
NCGGTGACCCGGGAGATCTGAATTCAGATCGGAAGA308320.10364145837166133No Hit
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTT305050.10254225115553738Illumina Paired End PCR Primer 2 (96% over 25bp)
ATTCAGATCTCCCGGGTCACCGCAGATCGGAAGAGC303630.10206491958156308No Hit
GAATTCAGATCTCCCGGGTCACCACAGATCGGAAGA303140.10190020657364236No Hit
GGTGACCCGGGAGATCTGAATTCAGATCGGAAGAGC301970.1015069122486072No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGTGA11201500.030.0629521
ACCGCAG9524350.028.68717421
CCGCAGA9516000.028.67105122
CGCAGAT9520150.028.63447223
CACCGCA9625750.028.58444820
GCAGATC9521250.028.57812724
ACCGAGA738950.028.24825121
CACCGAG749750.028.16942820
TATGCCG16300.027.878053
CGGTGAC12361300.027.1774482
ACCGCAA59250.027.03220721
TCACCGC10209900.026.99990519
ACCGCGA18400.026.9779421
GGTGACC12461400.026.9574533
GGGTCAC11136900.026.95251316
GTCACCG11024300.026.93714318
CGGGTCA11500600.026.90803115
GGTCACC11117150.026.88051617
CCGGGTC11517050.026.87589514
CCGCAAG40100.026.85329822
Produced by FastQC (version 0.11.3)