##FastQC	0.11.3
>>Basic Statistics	pass
#Measure	Value
Filename	V503_H3K4me3_ChIPSeq.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	49947198
Sequences flagged as poor quality	0
Sequence length	36
%GC	66
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.19756209347319	36.0	28.0	38.0	2.0	40.0
2	28.375325138359113	36.0	23.0	38.0	2.0	40.0
3	28.280795891693465	36.0	23.0	38.0	2.0	40.0
4	28.17842996918466	36.0	22.0	38.0	2.0	40.0
5	26.327640661644324	33.0	14.0	38.0	2.0	40.0
6	26.43381190272175	33.0	13.0	39.0	2.0	40.0
7	26.26061642136562	33.0	12.0	39.0	2.0	40.0
8	25.968291454507618	33.0	11.0	39.0	2.0	40.0
9	26.188629880699214	33.0	11.0	39.0	2.0	40.0
10	26.078263689586752	33.0	11.0	39.0	2.0	40.0
11	27.668234222067873	36.0	14.0	39.0	2.0	40.0
12	27.65603892334461	36.0	15.0	39.0	2.0	40.0
13	27.60962492830929	36.0	14.0	39.0	2.0	40.0
14	27.54011924753016	36.0	13.0	39.0	2.0	40.0
15	27.310529071120264	35.0	8.0	39.0	2.0	40.0
16	27.377259601229284	36.0	2.0	39.0	2.0	40.0
17	27.2564058348178	35.0	2.0	39.0	2.0	40.0
18	27.121999516369268	35.0	2.0	39.0	2.0	40.0
19	27.042120581018377	35.0	2.0	39.0	2.0	40.0
20	26.877559017424762	35.0	2.0	39.0	2.0	40.0
21	26.72659827684428	35.0	2.0	39.0	2.0	40.0
22	26.472707037539923	35.0	2.0	39.0	2.0	40.0
23	26.427871409323103	35.0	2.0	39.0	2.0	40.0
24	26.27751648851253	35.0	2.0	39.0	2.0	40.0
25	26.00644832969409	34.0	2.0	39.0	2.0	40.0
26	25.42546602914542	33.0	2.0	38.0	2.0	40.0
27	25.14755244128009	33.0	2.0	38.0	2.0	40.0
28	24.847444315094513	33.0	2.0	38.0	2.0	40.0
29	24.717669928150926	33.0	2.0	38.0	2.0	39.0
30	24.4413439969145	33.0	2.0	38.0	2.0	39.0
31	24.40910579208067	33.0	2.0	38.0	2.0	39.0
32	24.41389911402037	33.0	2.0	38.0	2.0	39.0
33	24.403807556932424	33.0	2.0	38.0	2.0	39.0
34	24.28925790792108	33.0	2.0	38.0	2.0	39.0
35	24.152697454619975	33.0	2.0	38.0	2.0	39.0
36	24.05855641791958	33.0	2.0	38.0	2.0	39.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1	1	0.13273886151547387
1	2	0.25880955764276337
1	3	0.27577565396933323
1	4	0.45812631430431594
1	5	2.3371399727091067
1	6	2.1030896176205616
1	7	2.145639190410062
1	8	2.2095308418684105
1	9	2.0998584470150696
1	10	2.1804143733844334
1	11	0.6822445480264676
1	12	0.6437472027473277
1	13	0.6193453049848507
1	14	0.6204323230447599
1	15	0.7645897617667536
1	16	0.7957678171849487
1	17	0.7866960280300361
1	18	0.8483843299066542
1	19	0.8407019956312993
1	20	0.9070699921253684
1	21	1.0292453134972916
1	22	1.107420597887117
1	23	1.0776630685755606
1	24	1.0933611278798452
1	25	1.271451306414658
1	26	1.3858559385783344
1	27	1.6797026958056946
1	28	1.8684500308004424
1	29	1.7676796777084895
1	30	1.9632789532846644
1	31	1.8613947183658723
1	32	1.760453670746923
1	33	1.7067179210941248
1	34	1.6806814231617544
1	35	1.69715595511909
1	36	1.843097687281258
2	1	-0.30685368373642774
2	2	9.508278014394023E-4
2	3	-0.03397942684264876
2	4	0.11375596048589998
2	5	2.0897830078929758
2	6	1.9057713900372022
2	7	1.8416368491510227
2	8	2.0618029189094855
2	9	1.8522936994236296
2	10	1.9653643349691237
2	11	0.4615181519483045
2	12	0.36067739586644976
2	13	0.3916472955238284
2	14	0.40707793546008375
2	15	0.575412982311267
2	16	0.5583396684392739
2	17	0.6071923613910002
2	18	0.6257486540483299
2	19	0.6811296921844701
2	20	0.6970909180617859
2	21	0.7845944753807537
2	22	0.875520854177136
2	23	0.8601860482493358
2	24	0.9139810629284213
2	25	0.6886452219333741
2	26	1.319652314224001
2	27	1.0010947416266731
2	28	1.5817603268161946
2	29	1.3241240009244208
2	30	1.471554322027508
2	31	1.618093371653547
2	32	1.4831694800292752
2	33	1.4548999045593263
2	34	1.5048349540325852
2	35	1.4900984516933526
2	36	1.6607536440027353
3	1	0.06452159432357263
3	2	0.22969579190370126
3	3	0.33047642966998936
3	4	0.3891586695085003
3	5	2.3923523890592655
3	6	2.186467056064444
3	7	2.10962787066574
3	8	2.308348311612967
3	9	2.1227593318966456
3	10	2.2288075525766935
3	11	0.7412110377162797
3	12	0.6669084386067823
3	13	0.653115830921287
3	14	0.6375250530631256
3	15	0.835953426913477
3	16	0.8056060295131608
3	17	0.7739650905291775
3	18	0.8843265466635977
3	19	0.8554058199809909
3	20	0.9725872917913847
3	21	0.9419281342058845
3	22	1.131741682391219
3	23	1.1367528346685134
3	24	1.1099103508449453
3	25	1.23792292847617
3	26	1.2423682545336092
3	27	1.070901480366956
3	28	1.1243641390379189
3	29	1.0869199704192596
3	30	1.042664173843427
3	31	1.2492167984158513
3	32	1.5260808789711149
3	33	1.4568471309709246
3	34	1.3924105313047583
3	35	1.453502128710788
3	36	1.5799191997418696
4	1	0.16573641462254685
4	2	0.4878402217171889
4	3	0.5222023263888289
4	4	0.6841003991623467
4	5	2.5824182855337874
4	6	2.4177349600703657
4	7	2.3955706099745058
4	8	2.6243063138493596
4	9	2.4188922406640962
4	10	2.442671036392806
4	11	0.9744041077121608
4	12	0.9126851237036107
4	13	0.913501511275232
4	14	0.8906010426535467
4	15	1.0778310033379128
4	16	1.0588786402279275
4	17	1.1077826939871116
4	18	1.110562608833309
4	19	1.1466674511281312
4	20	1.2650193741396123
4	21	1.3037650834060877
4	22	1.4418714232560923
4	23	1.443605584892989
4	24	1.4558636239517178
4	25	1.6312283466735629
4	26	1.758822641928571
4	27	1.6092411150114785
4	28	1.8296234555572504
4	29	2.053914500081067
4	30	2.0021369793170685
4	31	2.118325867116681
4	32	1.8941569447156148
4	33	1.9574454031268083
4	34	1.8544416070600924
4	35	1.9179789245927097
4	36	1.8598191718997619
5	1	0.24664449534839505
5	2	0.5370238925669426
5	3	0.34187041955099673
5	4	0.6748882387856945
5	5	2.5820158966026057
5	6	2.445286039884685
5	7	2.4498090202909886
5	8	2.5701360033821743
5	9	2.425055121821565
5	10	2.489381039560996
5	11	0.8923773022991277
5	12	0.9265675498658013
5	13	0.9631683092232919
5	14	1.0034586873839508
5	15	1.1471336395644371
5	16	1.0545932409049321
5	17	1.1315821050758537
5	18	1.0906259392119004
5	19	1.1306954140748608
5	20	1.2820389841899384
5	21	1.4210273612134152
5	22	1.5053184508805515
5	23	1.4808310112098901
5	24	1.4984712970603233
5	25	1.6201707909825735
5	26	1.8589196189427994
5	27	1.564842574887571
5	28	2.0791474261486833
5	29	2.0484243378668054
5	30	2.307354409224317
5	31	2.1772072333618944
5	32	1.9936560140739275
5	33	2.1097885049606546
5	34	2.1030094081916317
5	35	2.0883365074575373
5	36	2.206995163036158
6	1	0.23757388150431424
6	2	0.46169365671439166
6	3	0.5627362461522125
6	4	0.7115833392025337
6	5	2.556287183750257
6	6	2.3695647539564995
6	7	2.3366884535883976
6	8	2.3524506101579945
6	9	2.2457610147488047
6	10	2.3949124259470587
6	11	0.9203347151711938
6	12	0.8659453680977052
6	13	0.8685279586524217
6	14	0.8848108344576744
6	15	1.0408637485175198
6	16	1.0383087260299106
6	17	1.024414859214417
6	18	1.0558411914331778
6	19	1.0006084367095553
6	20	1.159932169876516
6	21	1.2604204712586267
6	22	1.4022558670460157
6	23	1.3411801934530594
6	24	1.3471745062798988
6	25	1.5554082624080792
6	26	1.6663157726472022
6	27	1.5181699080499627
6	28	1.755217447646384
6	29	1.7562516744291194
6	30	1.6091887058249306
6	31	1.9279246076045382
6	32	1.859845574848869
6	33	1.8341554231554227
6	34	1.8266156814562748
6	35	1.8075208892636034
6	36	1.988755020069803
7	1	4.6143445716140263E-4
7	2	0.21933815436092985
7	3	0.34861094048036634
7	4	0.5157856068122868
7	5	2.411087006180338
7	6	2.1947445662015177
7	7	2.1653178641428497
7	8	2.466577547889404
7	9	2.2910216204028657
7	10	2.339408809178199
7	11	0.8505682558971372
7	12	0.7973729007167378
7	13	0.7507316637871817
7	14	0.7326226252572816
7	15	0.9758663634007405
7	16	0.9228558516296843
7	17	0.9422549747894635
7	18	0.9595316805589746
7	19	0.9056471643388626
7	20	1.0570330276408981
7	21	1.0062084052903586
7	22	1.3148011728189353
7	23	1.2274037018876918
7	24	1.2051966031610029
7	25	1.3987678212863095
7	26	1.6861036145573287
7	27	1.4986180352406748
7	28	1.8472698091733655
7	29	1.7813673866475135
7	30	1.8794419769788107
7	31	1.8330471333587681
7	32	1.8990743207544085
7	33	1.8104729059166047
7	34	1.8655149142557477
7	35	1.79619088000479
7	36	1.9730006354981278
8	1	0.24806489891051697
8	2	0.5497329496986438
8	3	0.5770462558003544
8	4	0.7423094302315789
8	5	2.6575265986324546
8	6	2.494644694231276
8	7	2.3817227939787493
8	8	2.6684354404826784
8	9	2.4863805634566134
8	10	2.4999460891266274
8	11	1.0341345222083973
8	12	0.9154598844490991
8	13	0.9762565210396197
8	14	0.9164251400858241
8	15	1.1193449223075262
8	16	1.1184650241267207
8	17	1.1189434303799892
8	18	1.1156134525950208
8	19	1.1815030675157203
8	20	1.2853431132568893
8	21	1.3052683211739584
8	22	1.3854623155993053
8	23	1.3825923631922166
8	24	1.397928287620065
8	25	1.5568160076009505
8	26	1.6200371861944234
8	27	1.4890248543810074
8	28	1.838719067091052
8	29	1.4947460611319272
8	30	1.5765934822825294
8	31	1.8048579127579103
8	32	1.8524397022475902
8	33	1.657883113393524
8	34	1.723140656179055
8	35	1.5724632319909126
8	36	1.8995462970568155
9	1	0.43093121431033055
9	2	0.5978566518038484
9	3	0.6707792357691531
9	4	0.8546097298427675
9	5	2.78933020457594
9	6	2.5972783483296737
9	7	2.5303897320316473
9	8	2.669599267764678
9	9	2.5949334635704098
9	10	2.639305159135933
9	11	1.1365332615081272
9	12	1.062550136837146
9	13	1.108137047001879
9	14	1.0205770921267856
9	15	1.2771775891374872
9	16	1.1810467447789499
9	17	1.269846783229987
9	18	1.2854047313169161
9	19	1.32881652976522
9	20	1.3978930156027367
9	21	1.4293559347017215
9	22	1.5630916699195367
9	23	1.5332931335547713
9	24	1.5389947119722436
9	25	1.615613392273442
9	26	1.574542128853615
9	27	1.6225591084089963
9	28	1.8865687306605565
9	29	1.813208429003648
9	30	1.9726203091487307
9	31	1.9941514166833585
9	32	1.856785933019811
9	33	1.9513221586206058
9	34	1.9326348335352286
9	35	1.889626092485507
9	36	2.0663612455302527
10	1	0.3078902622620916
10	2	0.4863043747691336
10	3	0.47452847889315564
10	4	0.7690846601084722
10	5	2.670285604902535
10	6	2.4897982469160524
10	7	2.3732259364149115
10	8	2.554748269423584
10	9	2.5175400910462145
10	10	2.561745509223222
10	11	1.0989798187798563
10	12	1.0112846150169545
10	13	1.0486997333859733
10	14	0.898096421166052
10	15	1.176938477352703
10	16	1.1475189225266398
10	17	1.2132189486479383
10	18	1.1402822682568683
10	19	1.2738683291938173
10	20	1.193211884719144
10	21	1.4306353189646366
10	22	1.568984919958087
10	23	1.4534486600843586
10	24	1.4576022131545976
10	25	1.6820066191238006
10	26	1.9135250889849047
10	27	1.8493287807895626
10	28	2.157130851409203
10	29	1.997811184047002
10	30	1.6954795848797986
10	31	1.9085156467760847
10	32	2.0657795567756523
10	33	1.9564271404833846
10	34	2.022172804518952
10	35	2.0242594859752288
10	36	2.0878819013228025
11	1	0.6339657787142983
11	2	0.7545776993802491
11	3	0.8562358867538258
11	4	0.9697780688741986
11	5	2.786073715240306
11	6	2.639978247020302
11	7	2.534876708689911
11	8	2.6963673961785517
11	9	2.6299567074614636
11	10	2.594926048089153
11	11	1.1221534384760616
11	12	1.1164909355422203
11	13	1.115927734232205
11	14	1.142090731395843
11	15	1.2628320751023345
11	16	1.255530117828748
11	17	1.2819585420055049
11	18	1.330595619312426
11	19	1.3091614176061732
11	20	1.3705777797967507
11	21	1.4719699795940784
11	22	1.5423053243115064
11	23	1.4685746493379703
11	24	1.5180729515241609
11	25	1.7270003518340715
11	26	1.835155360537403
11	27	1.542126374507614
11	28	1.8445775336640686
11	29	1.7334383776130622
11	30	1.925012702467555
11	31	1.879833878281456
11	32	1.995918843880201
11	33	1.867083923943646
11	34	1.8279252007229054
11	35	1.6211228569911817
11	36	1.767173099533668
12	1	0.5262590222604437
12	2	0.669185145535355
12	3	0.6889376796544795
12	4	0.8173401638600524
12	5	2.605488901985428
12	6	2.449128371470554
12	7	2.3333110852902834
12	8	2.4750669070761013
12	9	2.37894284073856
12	10	2.4039921836357294
12	11	0.9730665922088235
12	12	0.9489215561037732
12	13	0.9873225585748742
12	14	0.9835108755467132
12	15	1.0150758220451763
12	16	0.9574997230423605
12	17	1.0255322826405298
12	18	1.06195834416242
12	19	0.9652238228129271
12	20	1.0867253120323959
12	21	1.080269348863279
12	22	1.1515264095719928
12	23	1.284897865319639
12	24	1.1126611303886413
12	25	1.2424689117264798
12	26	1.436926151029283
12	27	1.454169376766135
12	28	1.6493563172954673
12	29	1.5785473258434237
12	30	1.3933129241086988
12	31	1.6824870824094411
12	32	1.5679892547657772
12	33	1.5551956232448703
12	34	1.367265156902267
12	35	1.4591011292520513
12	36	1.4976291818776843
13	1	0.3775644163242724
13	2	0.5469200547355939
13	3	0.7329325324886504
13	4	0.7426779469522131
13	5	2.502399800694157
13	6	2.409380863782417
13	7	2.368043660653406
13	8	2.3296367902838924
13	9	2.281244295301512
13	10	2.311680346294086
13	11	0.9096741245283582
13	12	0.7480288238898893
13	13	0.8061942589176212
13	14	0.8834965556066585
13	15	0.8635990111828633
13	16	0.9739785053883168
13	17	0.989355188773839
13	18	0.9245197508903615
13	19	0.946987314181186
13	20	1.1080384857246734
13	21	1.157440585712628
13	22	1.203342601867483
13	23	1.1514997921230012
13	24	1.2269726160888332
13	25	1.2981701471111506
13	26	1.4964652275074357
13	27	1.3100010940229119
13	28	1.2430763438487737
13	29	1.479580578595062
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118	2	0.6274884067041135
118	3	0.7156815476864189
118	4	0.871645133486858
118	5	2.770998253609104
118	6	2.5737870976728914
118	7	2.5675842386609915
118	8	2.769759668066577
118	9	2.5715386717659783
118	10	2.6008275226504587
118	11	1.0972437485201212
118	12	0.9991285095031017
118	13	0.9219344041535713
118	14	0.9508009858010524
118	15	1.1671177513513662
118	16	1.0967115331858288
118	17	1.0801078810274447
118	18	1.1794478472758918
118	19	1.1886403825519452
118	20	1.2040396754407645
118	21	1.3708205513244742
118	22	1.4803964556204576
118	23	1.4771344875525898
118	24	1.5081755117733238
118	25	1.6824027838492306
118	26	1.886917709656771
118	27	2.156354577831774
118	28	2.3462843252952617
118	29	2.3313348230698807
118	30	2.511598474092942
118	31	2.3461989157278147
118	32	2.284179902183155
118	33	2.16476326558357
118	34	2.1161660517229137
118	35	2.212979827776415
118	36	2.231018534170662
119	1	0.6374951465210295
119	2	0.7557234143524667
119	3	0.9089424630073175
119	4	1.0493400430948903
119	5	2.8908820293384068
119	6	2.680624798516277
119	7	2.68320912113084
119	8	2.844488290350416
119	9	2.642927098632452
119	10	2.690123240463979
119	11	1.218707731194602
119	12	1.1364617463711362
119	13	1.1425995605342187
119	14	1.1480457777594566
119	15	1.2410853023813004
119	16	1.206783095307923
119	17	1.245121360960269
119	18	1.296413707156752
119	19	1.275895656669551
119	20	1.3313555828392758
119	21	1.4849462754292055
119	22	1.5518037539098088
119	23	1.5682345571827305
119	24	1.579202694665728
119	25	1.7338536844727273
119	26	2.0513628993216066
119	27	2.186861003723152
119	28	2.3499709290665365
119	29	2.3227728927016855
119	30	2.4891071805341234
119	31	2.4498210376429483
119	32	2.2496301342938736
119	33	2.1682834742172616
119	34	2.1682055093322283
119	35	2.132194059379721
119	36	2.1777975572097112
120	1	0.33566770198235574
120	2	0.3846850739323884
120	3	0.5528269533709818
120	4	0.6690862803265389
120	5	2.5058878806424048
120	6	2.3343095398005715
120	7	2.367657691625567
120	8	2.4852408293623682
120	9	2.3342897565516587
120	10	2.354200467922979
120	11	0.8938497223875679
120	12	0.8257750256021197
120	13	0.7877371687451067
120	14	0.7977129257063957
120	15	0.9446224176794651
120	16	0.8979541726417537
120	17	0.9401030923118192
120	18	0.9707063431835863
120	19	0.9707880179781547
120	20	0.9536757724122467
120	21	1.1465434866249957
120	22	1.2466565469225088
120	23	1.189030463500309
120	24	1.2857298617463258
120	25	1.4415838614317167
120	26	1.796845244760945
120	27	1.8355146893789396
120	28	2.137027489657193
120	29	2.0726368898677805
120	30	2.1689112541527287
120	31	2.2328911627320664
120	32	2.142553926069123
120	33	1.989842568404903
120	34	2.023599979289859
120	35	2.022545535851279
120	36	2.064172101694332
>>END_MODULE
>>Per sequence quality scores	fail
#Quality	Count
2	1.0032039E7
3	305659.0
4	504413.0
5	783226.0
6	174131.0
7	120829.0
8	137281.0
9	167763.0
10	181082.0
11	170757.0
12	185961.0
13	202990.0
14	213451.0
15	216948.0
16	221093.0
17	228384.0
18	235894.0
19	242253.0
20	265575.0
21	305741.0
22	356996.0
23	409433.0
24	463845.0
25	524812.0
26	597472.0
27	681046.0
28	779122.0
29	899903.0
30	1057224.0
31	1261985.0
32	1530017.0
33	1870064.0
34	2285098.0
35	2791179.0
36	3428304.0
37	4206365.0
38	4617187.0
39	7265386.0
40	26290.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.228560227855134	19.9670702171745	11.618440589533495	34.18592896543687
2	35.19562068732722	21.220138958524196	15.990314396623937	27.593925957524647
3	33.04701166005573	18.978480035648268	17.375774276748896	30.598734027547103
4	34.30300815876224	18.783995852665555	16.057160624045846	30.855835364526364
5	35.100044206219785	19.601085631553346	16.859119741522775	28.43975042070409
6	34.73338326484417	17.51821719228456	14.89580580364532	32.85259373922595
7	32.766398016116526	19.69154701750507	16.468328653006992	31.073726313371413
8	33.66145372225493	18.094463164933206	16.46725132608639	31.776831786725474
9	34.08051989253147	16.50992167962667	16.107210834067313	33.30234759377455
10	33.22804677073643	19.45658865428927	15.44762302463305	31.867741550341243
11	36.00386423795246	16.84497206449283	15.55920542873169	31.591958268823017
12	34.22499314004586	16.586381594763285	16.01716324100658	33.17146202418427
13	34.282637391350605	17.66185901681582	16.18086597559523	31.87463761623835
14	34.11560184891593	17.699323289327467	15.913742474909961	32.271332386846645
15	32.665198010848684	16.86497070785162	18.304205348450903	32.1656259328488
16	34.040651736879774	16.585461218096302	17.124659135082624	32.249227909941304
17	32.78558517447212	16.714508219399253	15.781245349150119	34.718661256978514
18	32.892141371508636	18.068866077522593	15.800267949249708	33.23872460171906
19	35.59010330024422	16.49194723589949	15.66905895505706	32.24889050879923
20	33.29930750879904	17.552468386066682	15.598002196571073	33.5502219085632
21	34.00325969297482	18.05619669892849	15.545945352648822	32.39459825544787
22	34.295998267157366	17.70661516723146	15.587470090245473	32.4099164753657
23	34.149657566640236	16.666948086571402	16.73373166195015	32.449662684838216
24	33.209239338097284	17.818412753240747	15.614699981059948	33.35764792760202
25	34.23906512587088	17.918022336839442	15.504488312910336	32.33842422437934
26	34.385977973671366	16.645655512780248	16.87841982844049	32.089946685107904
27	35.22305540777231	17.383019392389805	15.55925449969419	31.834670700143704
28	33.94452748189288	17.542851849768283	15.389644605109243	33.1229760632296
29	34.99739277968075	16.46163834136499	15.528082130834452	33.012886748119804
30	35.28983802711569	17.448587081586346	15.402732675773109	31.858842215524852
31	34.85397405253698	17.73538614210637	15.523000963074857	31.887638842281795
32	34.931165555642885	16.436918141998355	15.522977916849099	33.10893838550966
33	34.50976014534246	17.897563226676937	15.400297893851675	32.19237873412893
34	34.5799319723768	16.399180351914335	16.30385617820331	32.71703149750556
35	33.46761395808217	16.580656240772388	16.347326206325185	33.60440359482026
36	34.7636236824466	16.68199384204525	16.256821545920303	32.297560929587846
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	117321.0
1	103951.0
2	90581.0
3	90581.0
4	63100.0
5	35619.0
6	35619.0
7	33350.5
8	31082.0
9	31082.0
10	33257.5
11	35433.0
12	35433.0
13	38834.0
14	42235.0
15	46483.5
16	50732.0
17	50732.0
18	56853.0
19	62974.0
20	62974.0
21	74987.5
22	87001.0
23	87001.0
24	107554.5
25	128108.0
26	158293.5
27	188479.0
28	188479.0
29	229327.0
30	270175.0
31	270175.0
32	314044.5
33	357914.0
34	357914.0
35	402536.5
36	447159.0
37	447159.0
38	488651.5
39	530144.0
40	572777.0
41	615410.0
42	615410.0
43	674346.5
44	733283.0
45	733283.0
46	824873.5
47	916464.0
48	916464.0
49	1065353.5
50	1214243.0
51	1433134.0
52	1652025.0
53	1652025.0
54	1961764.5
55	2271504.0
56	2271504.0
57	3036159.5
58	3800815.0
59	3800815.0
60	3839764.5
61	3878714.0
62	3878714.0
63	4011493.5
64	4144273.0
65	4311380.0
66	4478487.0
67	4478487.0
68	4537506.0
69	4596525.0
70	4596525.0
71	4543459.5
72	4490394.0
73	4490394.0
74	4307996.5
75	4125599.0
76	3833571.0
77	3541543.0
78	3541543.0
79	3162470.0
80	2783397.0
81	2783397.0
82	2372411.0
83	1961425.0
84	1961425.0
85	1586689.5
86	1211954.0
87	1211954.0
88	928207.5
89	644461.0
90	463597.5
91	282734.0
92	282734.0
93	191026.0
94	99318.0
95	99318.0
96	62495.5
97	25673.0
98	25673.0
99	14836.5
100	4000.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.8506082763641716
2	1.0072476938546182
3	0.8745235318305544
4	0.8861958582741719
5	1.1040339039639422
6	0.9946403800269236
7	0.9557232820147388
8	1.1660113546309445
9	0.9574210749519924
10	1.0129517175317821
11	1.1646459126696156
12	1.0610364969822732
13	1.0016738076077862
14	1.0245679847746414
15	1.1322657178887192
16	1.0910121524734981
17	1.0560492302290911
18	1.0603818056019878
19	1.058768101465872
20	1.0705885843686367
21	1.0616691651051176
22	1.0983559077728444
23	1.1108010503412025
24	1.0467754367322066
25	1.0175605846798452
26	1.090884017157479
27	0.9554590029254495
28	0.9199434971307099
29	0.9087076316072825
30	0.8925946156178772
31	0.8999263582313467
32	0.9085754920626379
33	0.9070739063280387
34	0.902621204096374
35	0.8922242244700094
36	0.8968891508188307
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
36	4.9947198E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	68.62770571132272
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.54860300968595	54.592381170955676
2	10.60415098545163	14.554771062960143
3	4.857724312343764	10.00123423602796
4	2.3988288042610293	6.585044689226803
5	1.2106263459288578	4.154125429738982
6	0.6237764548012703	2.568500818185226
7	0.323332704962234	1.5532707206095886
8	0.17181880881746267	0.9433224517755878
9	0.08908969517400532	0.5502619244081774
>10	0.1653888741979046	1.6276415244378162
>50	0.004387727048855624	0.20734130059790623
>100	0.002120685939425306	0.28172255779214994
>500	7.68725946915803E-5	0.03358978840365481
>1k	4.558404977266462E-5	0.06745707564502487
>5k	5.826995931837907E-6	0.022567978308019543
>10k+	2.3307747015176014E-5	2.256767270927271
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTT	372120	0.7450267780787222	Illumina Paired End PCR Primer 2 (96% over 25bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCG	329783	0.6602632644177557	Illumina Paired End PCR Primer 2 (97% over 36bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCG	209400	0.4192427371000872	Illumina Paired End PCR Primer 2 (100% over 36bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	72921	0.1459961778036077	No Hit
CGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAA	64436	0.12900823785950916	Illumina Paired End PCR Primer 2 (96% over 33bp)
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.427844100483875E-4	2.002114312798888E-6	2.002114312798888E-6	2.0021143127988882E-5
2	0.0010290867567786285	4.004228625597776E-6	2.002114312798888E-6	3.40359433175811E-5
3	0.0023184483742211123	4.004228625597776E-6	4.004228625597776E-6	4.204440056877665E-5
4	0.0028369959812360244	4.004228625597776E-6	4.004228625597776E-6	5.6059200758368866E-5
5	0.0036918987928011497	4.004228625597776E-6	4.004228625597776E-6	7.407822957355886E-5
6	0.004258497143323235	4.004228625597776E-6	6.006342938396665E-6	8.40888011375533E-5
7	0.004939216009674857	4.004228625597776E-6	8.008457251195552E-6	1.1211840151673773E-4
8	0.005605920075836887	4.004228625597776E-6	8.008457251195552E-6	1.2613320170632995E-4
9	0.006596966660672336	4.004228625597776E-6	8.008457251195552E-6	1.4815645914711772E-4
10	0.007934379021621994	4.004228625597776E-6	8.008457251195552E-6	1.6016914502391106E-4
11	0.009890444705226507	4.004228625597776E-6	1.0010571563994441E-5	1.681776022751066E-4
12	0.012176859250442838	6.006342938396665E-6	1.0010571563994441E-5	1.7418394521350326E-4
13	0.013772544357743551	6.006342938396665E-6	1.0010571563994441E-5	1.841945167774977E-4
14	0.015003844660114868	6.006342938396665E-6	1.0010571563994441E-5	1.9820931696708992E-4
15	0.08189047962210012	6.006342938396665E-6	1.0010571563994441E-5	2.1022200284388326E-4
16	0.08474549463215134	6.006342938396665E-6	1.0010571563994441E-5	2.1823046009507882E-4
17	0.09100009974533506	6.006342938396665E-6	1.0010571563994441E-5	2.4025371753586658E-4
18	0.09521855460240232	6.006342938396665E-6	1.0010571563994441E-5	2.6227697497665435E-4
19	0.10204176018042094	8.008457251195552E-6	1.0010571563994441E-5	2.843002324174421E-4
20	0.9464274652604137	8.008457251195552E-6	1.0010571563994441E-5	2.9431080398143654E-4
21	0.9700123718651845	1.0010571563994441E-5	1.0010571563994441E-5	3.1433194710942544E-4
22	0.9749135477029162	1.201268587679333E-5	1.0010571563994441E-5	3.263446329862188E-4
23	0.9798207298835863	1.201268587679333E-5	1.0010571563994441E-5	3.4236154748860985E-4
24	0.9850282292111762	1.201268587679333E-5	1.0010571563994441E-5	3.58378461991001E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTATG	5750	0.0	24.482052	29
CTCGTAT	6010	0.0	23.496351	28
TCTCGTA	6250	0.0	22.375376	27
GCGGTTC	85580	0.0	21.461079	11
ATCTCGT	6875	0.0	20.126041	26
AGATCGG	65430	0.0	19.984226	20
CGTATGC	7845	0.0	17.98495	30
GACCGAT	8515	0.0	16.858883	21
GAGACCG	46265	0.0	16.542318	30
CGAGACC	46530	0.0	16.439177	29
CCGAGAC	47530	0.0	16.130245	28
ACCGATC	9005	0.0	15.908736	22
CCGATCT	9190	0.0	15.511883	23
TCGGAAG	119420	0.0	15.375108	3
ATCGGAA	121270	0.0	14.976609	2
AGCGGTT	122070	0.0	14.911185	10
CGGTTCA	123540	0.0	14.765382	12
TATGCCG	3535	0.0	14.734583	3
GATCGGA	123410	0.0	14.725169	1
TTCAGCA	127030	0.0	14.304269	15
>>END_MODULE
