FastQCFastQC Report
Sun 14 Aug 2016
V503_H3K4me3_ChIPSeq.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameV503_H3K4me3_ChIPSeq.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49947198
Sequences flagged as poor quality0
Sequence length36
%GC66

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTT3721200.7450267780787222Illumina Paired End PCR Primer 2 (96% over 25bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCG3297830.6602632644177557Illumina Paired End PCR Primer 2 (97% over 36bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCG2094000.4192427371000872Illumina Paired End PCR Primer 2 (100% over 36bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN729210.1459961778036077No Hit
CGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAA644360.12900823785950916Illumina Paired End PCR Primer 2 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG57500.024.48205229
CTCGTAT60100.023.49635128
TCTCGTA62500.022.37537627
GCGGTTC855800.021.46107911
ATCTCGT68750.020.12604126
AGATCGG654300.019.98422620
CGTATGC78450.017.9849530
GACCGAT85150.016.85888321
GAGACCG462650.016.54231830
CGAGACC465300.016.43917729
CCGAGAC475300.016.13024528
ACCGATC90050.015.90873622
CCGATCT91900.015.51188323
TCGGAAG1194200.015.3751083
ATCGGAA1212700.014.9766092
AGCGGTT1220700.014.91118510
CGGTTCA1235400.014.76538212
TATGCCG35350.014.7345833
GATCGGA1234100.014.7251691
TTCAGCA1270300.014.30426915
Produced by FastQC (version 0.11.3)