##FastQC	0.11.3
>>Basic Statistics	pass
#Measure	Value
Filename	V503_H3K27ac_ChIPSeq.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	41336972
Sequences flagged as poor quality	0
Sequence length	36
%GC	59
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.946860065125236	39.0	35.0	40.0	2.0	40.0
2	33.481681677119454	39.0	35.0	40.0	2.0	40.0
3	33.460757382035624	39.0	35.0	40.0	2.0	40.0
4	33.437601428571014	39.0	35.0	40.0	2.0	40.0
5	33.426848609036966	39.0	35.0	40.0	2.0	40.0
6	33.27027618278378	39.0	35.0	40.0	2.0	40.0
7	32.922552842041746	39.0	34.0	40.0	2.0	40.0
8	33.11766894778844	39.0	35.0	40.0	2.0	40.0
9	33.095413689227165	39.0	35.0	40.0	2.0	40.0
10	33.035562425810966	39.0	35.0	40.0	2.0	40.0
11	33.1336925694509	39.0	35.0	40.0	2.0	40.0
12	33.106596656378215	39.0	35.0	40.0	2.0	40.0
13	33.03182294049017	39.0	35.0	40.0	2.0	40.0
14	32.97845352581703	39.0	35.0	40.0	2.0	40.0
15	32.89150697830504	39.0	35.0	40.0	2.0	40.0
16	32.816988022247976	39.0	35.0	40.0	2.0	40.0
17	32.70883559153776	39.0	34.0	40.0	2.0	40.0
18	32.62216792270126	39.0	34.0	40.0	2.0	40.0
19	32.53473999014732	39.0	33.0	40.0	2.0	40.0
20	32.43680531317098	39.0	33.0	40.0	2.0	40.0
21	32.29107702905767	39.0	33.0	40.0	2.0	40.0
22	32.14824639308365	39.0	33.0	40.0	2.0	40.0
23	32.03543138089553	39.0	33.0	40.0	2.0	40.0
24	31.97673632214764	39.0	33.0	40.0	2.0	40.0
25	31.8852174029583	38.0	33.0	40.0	2.0	40.0
26	31.544745681904324	38.0	33.0	40.0	2.0	40.0
27	31.390512469079738	38.0	32.0	40.0	2.0	40.0
28	31.185285632435775	38.0	31.0	40.0	2.0	40.0
29	31.125376478954482	38.0	31.0	40.0	2.0	40.0
30	30.808493544229606	38.0	31.0	40.0	2.0	40.0
31	30.546196223564706	38.0	30.0	40.0	2.0	40.0
32	30.52312864135283	38.0	30.0	40.0	2.0	40.0
33	30.195546906532	38.0	29.0	40.0	2.0	40.0
34	30.067200374521867	38.0	29.0	39.0	2.0	40.0
35	30.33493824850064	38.0	30.0	40.0	2.0	40.0
36	30.261585851039115	38.0	30.0	40.0	2.0	40.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1	1	-0.696139992539436
1	2	-0.7797428942717559
1	3	-0.7023681056920594
1	4	-0.7413328517568445
1	5	-0.6964832499420126
1	6	-0.7457507693789722
1	7	-0.6249173285297402
1	8	-0.9225525592444654
1	9	-0.8475665407160022
1	10	-0.7623961788945692
1	11	-0.7173676915522051
1	12	-0.681240726085349
1	13	-0.6652937883394117
1	14	-0.6185285733995727
1	15	-0.5773607584733682
1	16	-0.4619712770605062
1	17	-0.49702019884736615
1	18	-0.46358686918089376
1	19	-0.45434630107041585
1	20	-0.41325772461842547
1	21	-0.3479703312142277
1	22	-0.4674865009980138
1	23	-0.20523190203240915
1	24	-0.28388569593303714
1	25	-0.1586506389527358
1	26	-0.043358478013402646
1	27	-0.06512006002516202
1	28	-0.0567558543564175
1	29	-0.003541432679430301
1	30	0.016587978876891896
1	31	0.03609276357881441
1	32	0.2124488966078779
1	33	0.08528902655170256
1	34	0.04862982374097058
1	35	0.20703633505476304
1	36	0.14005763265929616
2	1	-0.8838559621813857
2	2	-0.9830425335648911
2	3	-0.8819258566206898
2	4	-0.9482903596675101
2	5	-0.8981903112864842
2	6	-0.9728315438291233
2	7	-0.9664781649064658
2	8	-1.159098169005084
2	9	-1.0579459931985937
2	10	-1.2624335247070775
2	11	-0.9811343583854963
2	12	-0.8311376252365861
2	13	-0.9380281238451857
2	14	-0.8420208534950078
2	15	-0.7788052955067641
2	16	-0.6633735032147996
2	17	-0.68448386535362
2	18	-0.6935095517250147
2	19	-0.7563863297722229
2	20	-0.6249499827707012
2	21	-0.5671628431060824
2	22	-0.5730734860305411
2	23	-0.5066430791859524
2	24	-0.4778479416998067
2	25	-0.36557300440319906
2	26	-0.37203734484750584
2	27	-0.3509232637336872
2	28	-0.674843752905609
2	29	-0.14995786086799612
2	30	-0.2019557064829769
2	31	-0.19898781836143442
2	32	0.014786331842501
2	33	-0.1202698246141587
2	34	0.058698334387408124
2	35	0.01932789497895726
2	36	-0.003331350512745246
3	1	-0.7698042173722399
3	2	-0.8439082707062795
3	3	-0.7546694001279022
3	4	-0.8140173206231012
3	5	-0.7604180990941174
3	6	-0.7942771420245762
3	7	-0.8184943749959857
3	8	-0.7639112170910991
3	9	-0.7049762244267015
3	10	-0.8435716075236286
3	11	-0.8439262242853687
3	12	-0.7065289071050032
3	13	-0.7345279176116861
3	14	-0.6532242837293794
3	15	-0.6672340456517887
3	16	-0.49361898436196583
3	17	-0.5102236641783691
3	18	-0.5153091274615278
3	19	-0.5476119706691946
3	20	-0.4559070866911554
3	21	-0.4337023934214379
3	22	-0.3182051251167337
3	23	-0.38015179836386537
3	24	-0.26483363443236385
3	25	-0.23681674938656272
3	26	-0.15110952820401025
3	27	-0.12178432933336936
3	28	-0.3472823897872246
3	29	0.058456096476660946
3	30	-0.01965936938326962
3	31	0.18843823280479555
3	32	0.24504365367289438
3	33	0.09941507295302188
3	34	0.39338595453155634
3	35	0.3183152139208758
3	36	0.2528354565656983
4	1	-0.6309927189623465
4	2	-0.771822677518486
4	3	-0.6820928282746905
4	4	-0.691705063215089
4	5	-0.6447006203067787
4	6	-0.8408574235898243
4	7	-1.0451566900426812
4	8	-0.7182104247875998
4	9	-0.7102766838348131
4	10	-0.8296847551922326
4	11	-0.7354721935528516
4	12	-0.6792243673400904
4	13	-0.7088008348698267
4	14	-0.6097612983084275
4	15	-0.6492728578013214
4	16	-0.4582761600751226
4	17	-0.44338965544549325
4	18	-0.5013666166805493
4	19	-0.44265383205640774
4	20	-0.4601381553384698
4	21	-0.39641699431298605
4	22	-0.3509052934390269
4	23	-0.5499227447920845
4	24	-0.23821166524603044
4	25	-0.2085792320758877
4	26	-0.04938525078080147
4	27	-0.09666845068211316
4	28	-0.3409337251225679
4	29	0.051776143407504804
4	30	0.06578094003665313
4	31	0.15948241034762134
4	32	0.22559060184739366
4	33	0.06061320276912596
4	34	0.3330664406693771
4	35	0.2660459689031889
4	36	0.1769193973933625
5	1	-0.7072348072562065
5	2	-0.7482956028201855
5	3	-0.6581976576008444
5	4	-0.7578521891653622
5	5	-0.6807181378331393
5	6	-0.8148575419496638
5	7	-0.8332001469050851
5	8	-0.7790651862104099
5	9	-0.6779714216583912
5	10	-0.8146874425690669
5	11	-0.6979123735974966
5	12	-0.6554456845614709
5	13	-0.647171161119708
5	14	-0.6144686114249822
5	15	-0.5978422501291192
5	16	-0.48584349344953637
5	17	-0.5577524805351928
5	18	-0.4499200333455491
5	19	-0.5804504231302836
5	20	-0.4521576673953831
5	21	-0.41447420794647627
5	22	-0.27152365441093096
5	23	-0.37955833604786093
5	24	-0.24352327676555063
5	25	-0.20917502062683013
5	26	-0.03157070383856109
5	27	-0.023558103693925858
5	28	-0.12938134757094488
5	29	0.10859652355694749
5	30	0.14607315154796652
5	31	0.27193625546355804
5	32	0.25965857345191523
5	33	0.12747264399657965
5	34	0.39000218627811734
5	35	0.30918947899352744
5	36	0.2948123310992976
6	1	-0.5994783605812017
6	2	-0.6833631013455488
6	3	-0.6256853340494004
6	4	-0.6864804100755322
6	5	-0.6205011583754398
6	6	-0.686537989590775
6	7	-0.6570452406831038
6	8	-0.7231050197115749
6	9	-0.5893297413183163
6	10	-0.735044966541615
6	11	-0.6599032183025244
6	12	-0.6095942507027203
6	13	-0.6369048734336076
6	14	-0.5643360424215516
6	15	-0.5279797423228132
6	16	-0.40358671748204955
6	17	-0.437749105515735
6	18	-0.4061593844571405
6	19	-0.43550350389345027
6	20	-0.33181816606932557
6	21	-0.24840107183931082
6	22	-0.20772236311009706
6	23	-0.34334497546160847
6	24	-0.2514267091411675
6	25	-0.1791989494686277
6	26	0.06441767888083305
6	27	-0.09583075329347679
6	28	0.011963341014816109
6	29	0.10064706496143927
6	30	0.17753894216508925
6	31	0.16818899340682236
6	32	0.3296997190819795
6	33	0.27367358691105537
6	34	0.20146988787974962
6	35	0.386725390139528
6	36	0.4151178275156866
7	1	-0.5026328610250275
7	2	-0.5401648494339
7	3	-0.5561132393639454
7	4	-0.5596992196350428
7	5	-0.5241068752227918
7	6	-0.6079667625622065
7	7	-0.5064046154498811
7	8	-0.6260350719943659
7	9	-0.569767298260949
7	10	-0.6492630990213044
7	11	-0.6156307932383669
7	12	-0.5350736827342857
7	13	-0.6043102114692545
7	14	-0.5075580844691316
7	15	-0.504106832753024
7	16	-0.34663703332192597
7	17	-0.39234482447884034
7	18	-0.3291617627117631
7	19	-0.43834118598059035
7	20	-0.36390964418696115
7	21	-0.20987468637454754
7	22	-0.19261451734619683
7	23	-0.4959473247978039
7	24	-0.11456069018349879
7	25	-0.12905548018611057
7	26	0.07316677891915901
7	27	0.08746666491126476
7	28	0.015087582179649672
7	29	0.19093427913845673
7	30	0.09221235751560997
7	31	0.33619538879539945
7	32	0.4132681693793394
7	33	0.3672445303658485
7	34	0.20201436292575536
7	35	0.38536388702915403
7	36	0.267662702377649
8	1	-0.3930444070413799
8	2	-0.502723178535291
8	3	-0.4709644117081808
8	4	-0.4808183952582752
8	5	-0.438634771652481
8	6	-0.4774187823832534
8	7	-0.42750804015285837
8	8	-0.6437318639028717
8	9	-0.4743158989928986
8	10	-0.48235787250923323
8	11	-0.4580510411699592
8	12	-0.38104667710505424
8	13	-0.3752841060905965
8	14	-0.3598041731135879
8	15	-0.36594287211558196
8	16	-0.211062590345918
8	17	-0.17216859156274467
8	18	-0.23632897828219512
8	19	-0.19599170632092466
8	20	-0.21351385043927706
8	21	-0.09380585987211987
8	22	-0.09930948248205596
8	23	-0.19090007252397
8	24	-0.21761064096917693
8	25	0.05316190464228754
8	26	0.18007330574738134
8	27	0.17660003790731338
8	28	0.22428335536858413
8	29	0.30794765969728033
8	30	0.30999199944443845
8	31	0.3813270588402453
8	32	0.5621092710379259
8	33	0.5204041206959928
8	34	0.5311268189454168
8	35	0.5393507879657733
8	36	0.4238345891604318
9	1	-0.5351451047312707
9	2	-0.5847544026875227
9	3	-0.5311008361433949
9	4	-0.6688763456896183
9	5	-0.5023852238728495
9	6	-0.632636142111977
9	7	-0.452713773244632
9	8	-0.7619018319177542
9	9	-0.5060763482457631
9	10	-0.742289957830998
9	11	-0.5434200026557932
9	12	-0.42149539861294727
9	13	-0.4446898169386131
9	14	-0.4403136077213361
9	15	-0.3886692027063532
9	16	-0.27227722739876015
9	17	-0.21799764277530898
9	18	-0.21628912507058118
9	19	-0.3172474789637363
9	20	-0.19751131516418496
9	21	-0.12357306308723537
9	22	-0.12460962396393427
9	23	-0.07358226707890125
9	24	-0.08957616256400414
9	25	0.012449695589708654
9	26	0.1290397934737939
9	27	0.18127892171494508
9	28	-0.045475866384595776
9	29	0.3116046437083888
9	30	0.21573146225951945
9	31	0.4307667975545648
9	32	0.40687642165265814
9	33	0.2034422968765739
9	34	0.07723817856574655
9	35	0.46035822324598996
9	36	0.19792999344514328
10	1	-0.2587723913488844
10	2	-0.35730233449891813
10	3	-0.21011330311070253
10	4	-0.3008479331929905
10	5	-0.2550730147425426
10	6	-0.21974131746972603
10	7	-0.20202829854873272
10	8	-0.3358667858403095
10	9	-0.26520161421329647
10	10	-0.29726412455860185
10	11	-0.26813332177164995
10	12	-0.2128806832310417
10	13	-0.18728640560502186
10	14	-0.18243746978724573
10	15	-0.1574395986092938
10	16	-0.06100101703810168
10	17	-0.011608758640221595
10	18	-0.04099359906628308
10	19	-0.053044368159838484
10	20	-0.12688564043587292
10	21	-0.019599084366106467
10	22	0.013502864000429327
10	23	-0.12527204194611485
10	24	0.10515151435235737
10	25	0.21224377466413458
10	26	0.24836822509442769
10	27	0.28927586059258914
10	28	0.08936210593132188
10	29	0.422802186037071
10	30	0.3427236269076701
10	31	0.3725006705088667
10	32	0.4901389497914721
10	33	0.28693409498379907
10	34	0.5194815222048099
10	35	0.5554891577168455
10	36	0.3126376375904769
11	1	-0.38034739886580127
11	2	-0.47668494991255983
11	3	-0.3194979150593653
11	4	-0.38290566072689103
11	5	-0.38673827650261217
11	6	-0.39646360273046355
11	7	-0.2632132264395537
11	8	-0.5282689638305484
11	9	-0.39590241580001617
11	10	-0.47479877531667825
11	11	-0.36865008473554184
11	12	-0.30553254621020187
11	13	-0.2638340001868045
11	14	-0.2577097776225159
11	15	-0.22006812593493663
11	16	-0.12660175089663994
11	17	-0.16827977178940046
11	18	-0.2745824661808314
11	19	-0.1274288452779615
11	20	-0.1278082647946519
11	21	-0.030062368231199343
11	22	0.08506413536865409
11	23	0.07056278042868769
11	24	0.16214008793208023
11	25	0.08121733355193683
11	26	0.269441774984319
11	27	0.3109025914968804
11	28	0.22493765884466654
11	29	0.41889420034794256
11	30	0.3473963747844131
11	31	0.5698889738384914
11	32	0.5262281518348964
11	33	0.33503595097294436
11	34	0.40929329263776637
11	35	0.5025603147711664
11	36	0.4989536341094869
12	1	-0.39349991163964404
12	2	-0.4487711625022399
12	3	-0.35225422997601186
12	4	-0.39624779581802017
12	5	-0.35545344441867854
12	6	-0.4690727614909349
12	7	-0.33629734499114505
12	8	-0.507179978299213
12	9	-0.5106413398497125
12	10	-0.5269050935278514
12	11	-0.4361967352127962
12	12	-0.387322809091998
12	13	-0.38817772488290103
12	14	-0.43476339144788767
12	15	-0.3673498511564972
12	16	-0.24999645500113843
12	17	-0.26856557239362644
12	18	-0.22646991429611063
12	19	-0.2727599094757096
12	20	-0.43023684650114546
12	21	-1.8761773333211167
12	22	-2.1393492146507462
12	23	-2.748584764009795
12	24	-3.374294910570889
12	25	-4.048502864399364
12	26	-6.562044549328871
12	27	-6.7266637247204315
12	28	-7.157504801027983
12	29	-7.215664162655507
12	30	-7.560344497637615
12	31	-7.92498220441496
12	32	-7.706749385189848
12	33	-7.755139820864333
12	34	-7.748852742234423
12	35	-7.706391046320622
12	36	-7.551868156657726
13	1	-0.39400805340318357
13	2	-0.443429716196448
13	3	-0.3726257542260072
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118	3	-0.4517465597623769
118	4	-0.5160473353168342
118	5	-0.4725742841897258
118	6	-0.45055910473409
118	7	-0.5679001019221559
118	8	-0.47366424766396165
118	9	-0.4497413256402183
118	10	-0.5428874057375808
118	11	-0.4252990996445334
118	12	-0.4351669140465688
118	13	-0.4211083604856256
118	14	-0.3883844175530342
118	15	-0.351630283675469
118	16	-0.27117741354032887
118	17	-0.22418797614616892
118	18	-0.20510367002891883
118	19	-0.18701979357703635
118	20	-0.14033736774792516
118	21	-0.035687712834345575
118	22	0.0029188906206414345
118	23	-0.008806894956130407
118	24	0.017409987486935563
118	25	0.019435248137607886
118	26	0.21697677260825188
118	27	0.24202053667431755
118	28	0.24740828815445326
118	29	0.32747693418390966
118	30	0.4792356205938333
118	31	0.5152279778455267
118	32	0.514284572006833
118	33	0.6610639729874741
118	34	0.5524668685696135
118	35	0.6369049595052232
118	36	0.6506895161516688
119	1	-0.5001228035189058
119	2	-0.5225466276898203
119	3	-0.5086389969435317
119	4	-0.47626413521597755
119	5	-0.4616574792812429
119	6	-0.49166503582144827
119	7	-0.4043416553205148
119	8	-0.3546188777791244
119	9	-0.5033029907894431
119	10	-0.47907947660823424
119	11	-0.44289595148377003
119	12	-0.4588608612396996
119	13	-0.44471246383125873
119	14	-0.40896115166626856
119	15	-0.4421684213989394
119	16	-0.3115425477858196
119	17	-0.2591303158836453
119	18	-0.28217189157251
119	19	-0.2019067375841388
119	20	-0.2376111075472025
119	21	-0.08782539539979695
119	22	-0.15380355974365045
119	23	-0.11583187395732253
119	24	-0.05597160873928786
119	25	0.017727140876893088
119	26	0.13462934467983345
119	27	0.1668673305802777
119	28	0.18966558744161688
119	29	0.2821696319789879
119	30	0.40619058207627745
119	31	0.4763405530053326
119	32	0.5057659174051032
119	33	0.4985267563492606
119	34	0.5893132421793403
119	35	0.6011122856921638
119	36	0.5356104163594146
120	1	-0.5931396062235095
120	2	-0.5593842587716438
120	3	-0.573785053134209
120	4	-0.64040856297364
120	5	-0.6311019948550083
120	6	-0.5321318213006094
120	7	-0.4620410909635666
120	8	-0.5520210395937895
120	9	-0.6119662125497385
120	10	-0.5566414979850478
120	11	-0.5025972435444714
120	12	-0.5367364729989887
120	13	-0.5314600720495903
120	14	-0.459935295490979
120	15	-0.43238960477507504
120	16	-0.35704680860141025
120	17	-0.3022773049009686
120	18	-0.3148011278532792
120	19	-0.27389132914204595
120	20	-0.27878039806092403
120	21	-0.10449615288138148
120	22	-0.08684479038200266
120	23	-0.08530663835256291
120	24	-0.08098700575913398
120	25	-0.005527048110874233
120	26	0.08500737151124582
120	27	0.1295692339471266
120	28	0.06271657403339503
120	29	0.21318654436020523
120	30	0.3009821837075677
120	31	0.42689324459083977
120	32	0.49169636508305814
120	33	0.5928438052278011
120	34	0.6042253450914501
120	35	0.6274896219809705
120	36	0.625097037596408
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	4696325.0
3	49488.0
4	85217.0
5	133818.0
6	86683.0
7	68429.0
8	77157.0
9	78428.0
10	76678.0
11	67622.0
12	82163.0
13	93323.0
14	133571.0
15	113943.0
16	100192.0
17	108158.0
18	121493.0
19	120097.0
20	132543.0
21	164933.0
22	203682.0
23	221704.0
24	242244.0
25	265489.0
26	299132.0
27	337410.0
28	375903.0
29	394728.0
30	449102.0
31	533068.0
32	630677.0
33	766595.0
34	1003255.0
35	1456306.0
36	2253653.0
37	3818260.0
38	5530818.0
39	1.5729818E7
40	234867.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	30.13474868746517	23.468704476794773	14.658330318821308	31.738216516918744
2	31.23830490816587	23.1658511418993	20.412330962915632	25.183512987019196
3	29.336094864504474	22.029004700345155	20.3822499257242	28.252650509426168
4	31.467575455171403	21.71020788708386	19.390582015788265	27.43163464195647
5	31.39684493572184	22.791171395318482	19.971956137013912	25.840027531945765
6	31.33835398195812	21.179397281926963	18.39550882298794	29.086739913126973
7	30.28550417700821	21.34268013139243	20.60167801077864	27.77013768082072
8	30.03260466524579	20.234942091063715	20.706691253593704	29.025761990096793
9	30.11886799707508	20.068949711433575	20.563840554818288	29.248341736673055
10	30.473111466770487	21.445199993609616	19.237724898743462	28.843963640876435
11	31.153637513531894	20.572781464387315	19.47042255261258	28.803158469468208
12	30.816885330999057	20.32149829107454	19.72088446776421	29.14073191016219
13	30.23830981479252	20.736718516736357	19.996955651896464	29.028016016574664
14	30.144891850582063	20.7343946828995	19.90216529983495	29.21854816668349
15	29.79282113877365	20.48734631567603	20.340488166522334	29.379344379027984
16	30.201834777067194	20.393146236910706	20.060939795857436	29.344079190164663
17	29.977765884635726	20.427453952413646	19.81241470708492	29.782365455865705
18	30.117813555142646	20.543246483623847	19.782362020398274	29.556577940835233
19	30.691086070430078	20.30560449972917	19.797759487559258	29.20554994228149
20	30.331566476108573	20.700225782873524	19.65286262913725	29.315345111880653
21	30.49082148782815	20.533107855721294	19.683735142449432	29.292335514001117
22	30.339541519424905	20.671893882521722	19.749201314494723	29.23936328355865
23	30.298172781261005	20.552890933756185	19.992554544106444	29.156381740876363
24	30.18794223583931	20.67510174175373	19.69068722654131	29.44626879586565
25	30.360214840893462	20.6363482836595	19.6480528473867	29.35538402806034
26	30.361778115043876	20.601758513655387	19.815901087805575	29.220562283495166
27	30.481747686924482	20.72464596780204	19.740708296528155	29.052898048745323
28	30.46070016672876	20.791644609616174	19.593052563168094	29.154602660486965
29	30.468439953630245	20.564676330364232	19.580200186543696	29.386683529461827
30	30.611662146384578	20.734192459044735	19.471673430052025	29.182471964518662
31	30.910181165529487	20.58972651340156	19.50403220850835	28.996060112560603
32	30.445920749304968	20.454241156701364	19.568663549776847	29.53117454421682
33	30.679285054840594	20.717533292000006	19.519904344972193	29.083277308187206
34	30.855666837570716	20.47302338518639	19.735476788095852	28.935832989147038
35	30.21318687726647	20.58048979114819	19.77655955898165	29.429763772603696
36	30.40972518070616	20.728679007173326	19.885383592123855	28.976212219996654
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	91296.0
1	66872.0
2	42448.0
3	42448.0
4	35914.5
5	29381.0
6	29381.0
7	32874.0
8	36367.0
9	36367.0
10	43238.5
11	50110.0
12	50110.0
13	59439.0
14	68768.0
15	77652.0
16	86536.0
17	86536.0
18	94352.5
19	102169.0
20	102169.0
21	113787.0
22	125405.0
23	125405.0
24	146174.0
25	166943.0
26	204641.5
27	242340.0
28	242340.0
29	301071.0
30	359802.0
31	359802.0
32	439209.0
33	518616.0
34	518616.0
35	616126.0
36	713636.0
37	713636.0
38	833191.5
39	952747.0
40	1095762.0
41	1238777.0
42	1238777.0
43	1410668.0
44	1582559.0
45	1582559.0
46	1790890.5
47	1999222.0
48	1999222.0
49	2244382.0
50	2489542.0
51	2758420.5
52	3027299.0
53	3027299.0
54	3272938.5
55	3518578.0
56	3518578.0
57	3711764.0
58	3904950.0
59	3904950.0
60	3809717.5
61	3714485.0
62	3714485.0
63	3523709.0
64	3332933.0
65	3115798.0
66	2898663.0
67	2898663.0
68	2677315.5
69	2455968.0
70	2455968.0
71	2251033.0
72	2046098.0
73	2046098.0
74	1861570.5
75	1677043.0
76	1504717.0
77	1332391.0
78	1332391.0
79	1168706.5
80	1005022.0
81	1005022.0
82	851475.0
83	697928.0
84	697928.0
85	564789.0
86	431650.0
87	431650.0
88	333611.5
89	235573.0
90	171481.0
91	107389.0
92	107389.0
93	73960.0
94	40531.0
95	40531.0
96	26141.0
97	11751.0
98	11751.0
99	6903.5
100	2056.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	1.02516459115583
2	1.2997347749612622
3	1.2486158879755394
4	1.167434324894431
5	1.1393916322656628
6	1.3162599331175007
7	1.4553460761470385
8	0.9762834104055808
9	0.9687937471569035
10	1.423048112958056
11	1.3149076328087117
12	0.996299390289158
13	1.2905904186692727
14	1.2029666807718766
15	1.2530937195883627
16	1.14989554629207
17	1.1806234864034066
18	1.1775148890925053
19	1.2388788419238836
20	1.2512382377693265
21	1.3192524116183448
22	1.325116411526224
23	1.3362468832985637
24	1.2933893658200217
25	1.202944908494991
26	1.187522879034294
27	0.9967396741106243
28	1.2545185941534374
29	1.023860673684565
30	1.319085490828888
31	0.9897991560678417
32	1.023563119233794
33	1.2950948608427342
34	0.9906966577039072
35	1.0148082447838704
36	1.3312924807361313
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
36	4.1336972E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.99325623830278
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.6920735125221	74.49536056843655
2	7.992612600121237	13.426511162709412
3	2.1272660993554013	5.360280197106392
4	0.6690662844619845	2.2478822348489857
5	0.2570365547103583	1.079466860119883
6	0.10633849189354834	0.5359029718565693
7	0.044014927474116436	0.25878699571506425
8	0.02262989063427889	0.15206065621518042
9	0.014972528844842098	0.11318323066201816
>10	0.06618239971245621	1.0567068667026063
>50	0.005852582992892808	0.33202914772429953
>100	0.001880135670773683	0.2664126036535666
>500	3.6171377317263307E-5	0.020143754076492926
>1k	1.46938469052904E-5	0.03604318693898572
>5k	5.781595781644293E-6	0.03954606139415533
>10k+	1.7344785950518523E-5	0.5796835018397805
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTT	85804	0.2075720495444127	Illumina Paired End PCR Primer 2 (96% over 25bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	68199	0.16498305681412756	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.394686383898656E-4	2.419141876187738E-6	0.0	9.676567504750953E-6
2	6.313960296849997E-4	2.419141876187738E-6	2.419141876187738E-6	9.676567504750953E-6
3	9.458844735894057E-4	2.419141876187738E-6	4.838283752375476E-6	1.451485125712643E-5
4	0.0011418349655606124	2.419141876187738E-6	7.257425628563215E-6	2.902970251425286E-5
5	0.0014466468419602673	4.838283752375476E-6	7.257425628563215E-6	3.6287128142816074E-5
6	0.0016522739014362253	4.838283752375476E-6	7.257425628563215E-6	4.596369564756702E-5
7	0.0018820923796740603	4.838283752375476E-6	7.257425628563215E-6	5.322112127613024E-5
8	0.0020804620135214547	4.838283752375476E-6	1.209570938093869E-5	6.773597253325667E-5
9	0.0023997887411782363	4.838283752375476E-6	1.451485125712643E-5	7.741254003800762E-5
10	0.002719115468835018	4.838283752375476E-6	1.6933993133314168E-5	8.708910754275857E-5
11	0.003234392688463006	4.838283752375476E-6	1.6933993133314168E-5	9.434653317132178E-5
12	0.003788376178109998	7.257425628563215E-6	1.9353135009501906E-5	1.0886138442844822E-4
13	0.0043641319446426794	7.257425628563215E-6	1.9353135009501906E-5	1.1611881005701143E-4
14	0.004816511475489786	7.257425628563215E-6	2.1772276885689643E-5	1.2095709380938691E-4
15	0.015470412298220586	7.257425628563215E-6	2.1772276885689643E-5	1.3547194506651335E-4
16	0.016336465089895797	7.257425628563215E-6	2.1772276885689643E-5	1.3789108694270107E-4
17	0.017848428762513132	9.676567504750953E-6	2.419141876187738E-5	1.4756765444745202E-4
18	0.01892252775554049	9.676567504750953E-6	2.661056063806512E-5	1.4998679632363977E-4
19	0.020763494723319356	9.676567504750953E-6	2.661056063806512E-5	1.5966336382839072E-4
20	0.24364387406024804	9.676567504750953E-6	2.661056063806512E-5	1.7901649883789262E-4
21	0.24842409840759502	9.676567504750953E-6	2.661056063806512E-5	1.8627392446645584E-4
22	0.2501489465653169	9.676567504750953E-6	2.902970251425286E-5	1.9111220821883132E-4
23	0.2525946990021427	9.676567504750953E-6	3.14488443904406E-5	2.0320791759977002E-4
24	0.25670240190790944	1.209570938093869E-5	3.3867986266628337E-5	2.2014191073308417E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCGTAT	2410	0.0	19.520046	28
TCTCGTA	2475	0.0	19.132633	27
TCGTATG	2535	0.0	18.785278	29
GCGGTTC	12965	0.0	16.12669	11
GACCGAT	3045	0.0	15.871463	21
ATGCCGA	21810	0.0	15.844888	14
AATGCCG	22375	0.0	15.674479	13
CGGTTCA	22255	0.0	15.599144	1
ACCGATC	3170	0.0	15.300946	22
ATCTCGT	3240	0.0	14.661004	26
AGCGGTT	20575	0.0	14.09949	30
AGATCGG	20955	0.0	14.01956	20
GAATGCC	26250	0.0	13.538648	12
TGCCGAG	26045	0.0	13.41079	15
GTTCAGC	26295	0.0	13.329499	3
CGTATGC	3740	0.0	13.0147085	30
ATCGGAA	22810	0.0	12.949533	22
GAGCGGT	23075	0.0	12.746982	29
CGAGATC	23600	0.0	12.689458	18
AAGAGCG	23315	0.0	12.663258	27
>>END_MODULE
