FastQCFastQC Report
Sun 14 Aug 2016
V503_H3K27ac_ChIPSeq.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameV503_H3K27ac_ChIPSeq.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41336972
Sequences flagged as poor quality0
Sequence length36
%GC59

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTT858040.2075720495444127Illumina Paired End PCR Primer 2 (96% over 25bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN681990.16498305681412756No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT24100.019.52004628
TCTCGTA24750.019.13263327
TCGTATG25350.018.78527829
GCGGTTC129650.016.1266911
GACCGAT30450.015.87146321
ATGCCGA218100.015.84488814
AATGCCG223750.015.67447913
CGGTTCA222550.015.5991441
ACCGATC31700.015.30094622
ATCTCGT32400.014.66100426
AGCGGTT205750.014.0994930
AGATCGG209550.014.0195620
GAATGCC262500.013.53864812
TGCCGAG260450.013.4107915
GTTCAGC262950.013.3294993
CGTATGC37400.013.014708530
ATCGGAA228100.012.94953322
GAGCGGT230750.012.74698229
CGAGATC236000.012.68945818
AAGAGCG233150.012.66325827
Produced by FastQC (version 0.11.3)