FastQCFastQC Report
Sun 14 Aug 2016
V481_input.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameV481_input.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33116522
Sequences flagged as poor quality0
Sequence length36
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATTCAGATCTCCCGGGTCACCGCAGATCGGAAGA1000326630.20626984923115No Hit
GCGGTGACCCGGGAGATCTGAATTCAGATCGGAAGA750866822.673480023053145No Hit
CGGTGACCCGGGAGATCTGAATTCAGATCGGAAGAG6890412.0806562959721435No Hit
GCGGTGACCCGGGAGATCTGAATTCGATCTGAATTC6817652.058685389727822No Hit
GAATTCAGATCGAATTCAGATCTCCCGGGTCACCGC6257601.889570408390108No Hit
GAATTCAGATCTCCCGGGTCACCGAGATCGGAAGAG5984881.807218765303917No Hit
TGACCCGGGAGATCTGAATTCAGATCGGAAGAGCGG2218590.6699344816463516No Hit
GAATTCAGATCGAATTCAGATCGAATTCAGATCTCC2141850.6467617583754719No Hit
GAATTCAGATCTCCCGGGTCAAGATCGGAAGAGCGG1674950.5057747308126137No Hit
AATTCAGATCTCCCGGGTCACCGCAGATCGGAAGAG1289630.38942193265343505No Hit
GTGACCCGGGAGATCTGAATTCAGATCGGAAGAGCG795310.24015505009855803No Hit
GCGGTGACCCGGGAGATCTGAATTAGATCGGAAGAG696830.21041762779316014No Hit
GAATTCAGATCTCCCGGGTCACAGATCGGAAGAGCG587830.17750354339746185No Hit
GGTGACCCGGGAGATCTGAATTCAGATCGGAAGAGC575880.17389507267701604No Hit
GCGGTGACCCGGGAGATCTGAATTCAAGATCGGAAG537710.1623691038569811No Hit
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTT502630.1517762040349527Illumina Paired End PCR Primer 2 (96% over 25bp)
ATTCAGATCTCCCGGGTCACCGCAGATCGGAAGAGC497030.15008520520361407No Hit
GAATTCAGATCTCCCGGGTCACCAGATCGGAAGAGC486810.1469991323364211No Hit
GAATTCAGATCTCCCGGATCACCGCAGATCGGAAGA476710.14394929515847105No Hit
GAATTCAGATCTCCCGGTCACCGCAGATCGGAAGAG461640.13939869651770798No Hit
GAATTCAGATCTCCCGAGTCACCGCAGATCGGAAGA458160.13834786152966183No Hit
CGGTGACCCGGGAGATCTGAATTCGATCTGAATTCA455900.1376654227155859No Hit
GCGGTGACCGGGAGATCTGAATTCAGATCGGAAGAG438420.13238709064919316No Hit
GAATTCAGATCGAATTCAGATCTCCCGGGTCACCGA395970.11956871557949232No Hit
GAATTCAGATCTCCCAGGTCACCGCAGATCGGAAGA394740.11919729976475188No Hit
NAATTCAGATCTCCCGGGTCACCGCAGATCGGAAGA388730.11738249566183309No Hit
GAATTCAGATCTCCGGGTCACCGCAGATCGGAAGAG386710.11677252822624308No Hit
GAATTCAGATCTCCCGGGTCACCACAGATCGGAAGA384990.1162531500137605No Hit
GCGGTGACCCGGAGATCTGAATTCAGATCGGAAGAG356160.10754752567313682No Hit
GACCCGGGAGATCTGAATTCAGATCGGAAGAGCGGT331400.10007089512600387No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGTGA12980450.030.0417671
GCGGCGA34300.028.5712011
ACCGCAG11156350.028.48624221
CCGCAGA11155950.028.45884522
CGCAGAT11148050.028.42644523
CACCGCA11267900.028.39160220
GCAGATC11147800.028.38568924
ACCGAGA694500.028.23970421
GCGGTAC11250.028.2339481
CACCGAG708050.028.13041520
CCGCGAT21200.028.08550322
GCGATCG15650.028.07916324
ACCGCGA26350.027.88943721
CGCCGAT24100.027.81766523
CGGTGAC14056200.027.662622
TATGCCG17200.027.640713
ACCGCCG27400.027.47751421
GGTGACC14227250.027.335063
GCGTGAC27100.027.2187671
GTGACCC14317450.027.0344184
Produced by FastQC (version 0.11.3)