FastQCFastQC Report
Sun 14 Aug 2016
V481_H3K4me1_ChIPSeq.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameV481_H3K4me1_ChIPSeq.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9517017
Sequences flagged as poor quality0
Sequence length71
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGAT7250.012.58509852
TCGTATG6000.011.92040760
ACCGATC6950.011.69295353
ACGCCGA3351.5021324E-611.64124765
TCGCGAC2050.005810398611.10092663
TCTCGTA6153.6379788E-1211.09798358
CTCGTAT6355.456968E-1210.74872659
TATGCCG4656.27133E-810.48705163
ATGCCGA44400.010.02768465
CGATCGG22050.010.0224264
AATGCCG49300.010.01981565
GCGATCG8900.09.86254163
CGGACGA20300.09.77011162
ACGCGCG6701.7644197E-109.70443463
ACGAGCG16450.09.68039865
ACGCTCG3705.668036E-59.66509263
TGCCGAG44100.09.58025564
AAGCGCG13300.09.29015661
CGCGACC4202.2305383E-59.28547764
TCGATCG6357.1140676E-99.21216865
Produced by FastQC (version 0.11.3)