FastQCFastQC Report
Sun 14 Aug 2016
V457_input.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameV457_input.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21739055
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTTCA11000.010.4849211
ATGCCGA11350.010.16597214
AATGCCG12550.09.20125413
GAGCGGT10500.08.77026229
AGAGCGG13450.08.6425228
TGCCGAG13250.08.5921315
AGCGGTT10450.08.52329830
CCGATCT5100.08.2234671
AAGAGCG15900.07.310930727
TCGGAAG16350.07.144618523
TTGGCGC3804.421927E-66.70088961
TTGGGCG5853.3287506E-106.6570931
ATCGGAA19350.06.34651222
CGGAAGA17600.06.29681924
TTGGAAT88900.06.0823661
CCTGTTT75400.05.76094531
GATCGGA19900.05.719560621
CCGGCTC7602.285742E-85.3212951
CCTAATT51550.05.28822471
AGATCGG24350.05.26977430
Produced by FastQC (version 0.11.3)