FastQCFastQC Report
Sun 14 Aug 2016
V457_H3K4me1_ChIPSeq.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameV457_H3K4me1_ChIPSeq.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5808745
Sequences flagged as poor quality0
Sequence length35
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAGTC5457.1686372E-96.3703954
ACGGAGT6051.0504664E-85.9773163
GAGACGA3350.00276565065.61798762
GACGGAG7007.4414856E-95.58403252
AGACGGA6852.6280759E-85.49891471
TGAGACG4855.296056E-55.3768141
CGTCTCC4853.0372915E-45.0781021
Produced by FastQC (version 0.11.3)