FastQCFastQC Report
Sun 14 Aug 2016
V432_input.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameV432_input.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34247725
Sequences flagged as poor quality0
Sequence length36
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGA24350.014.35089814
AGCGGTT26350.013.26191830
GAGCGGT26600.013.02454829
AATGCCG27200.012.84780113
CGGTTCA27550.012.6289331
AGAGCGG31050.011.30286228
ATCGGAA32100.010.88621322
GATCGGA32250.010.83570621
AAGAGCG33100.010.55752527
TCGGAAG34300.010.36288823
TGCCGAG35350.010.22493515
CGGAAGA37500.09.63856424
TATGCCG8350.09.1603133
AGATCGG38100.09.09320120
CGTATGC10650.07.63695861
GCCGTCT17150.05.684116
CGAGATC72900.05.678218
GTTCAGC69950.05.55313973
ATGCCGT14950.05.5174364
GAATGCC72150.05.30082112
Produced by FastQC (version 0.11.3)