FastQCFastQC Report
Sun 14 Aug 2016
V432_H3K4me1_ChIPSeq.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameV432_H3K4me1_ChIPSeq.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15907952
Sequences flagged as poor quality0
Sequence length142
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAT33700.024.29745570-71
GCGCGAA17650.023.09027772-73
ATCGGAG54800.022.8565670-71
AGCGGTC48250.021.38741970-71
GATCGAA20250.020.3102472-73
GCGCGTT16900.019.91146572-73
CGGGTCG18800.019.88789270-71
GATCGTC24100.019.8582272-73
GATCGTT22350.019.74021372-73
ACGCGAA12600.019.28813272-73
CGCGTAA7050.019.0924172-73
ACGCGTT12050.018.92736472-73
AGCGCGT36250.018.66883370-71
CGCGAAA13450.018.62515472-73
GCGCGTA14500.018.56572372-73
GATCGAT19950.018.55408972-73
AGCGGTA41450.018.49166170-71
CGACACG20250.017.90990670-71
AGCGCGA35100.017.89570470-71
AGCGCGC49100.017.81890370-71
Produced by FastQC (version 0.11.3)