FastQCFastQC Report
Sun 14 Aug 2016
V432_DNase.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameV432_DNase.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26144874
Sequences flagged as poor quality0
Sequence length20
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGTATGCCGTCTTCTGCTT3614511.3824927976321477No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT386650.013.6756657
TGCCGTC388050.013.63030056
CCGTCTT389000.013.6159978
CGTCTTC389300.013.5983919
ATGCCGT389950.013.5739285
TATGCCG390200.013.5433944
CGTATGC399650.013.231732
GTATGCC401050.013.209343
TCGTATG404050.013.1092561
GTCTTCT421250.012.67843810
CTTCTGC430700.012.42277912
TCTGCTT432800.012.39453414
TTCTGCT434900.012.338140513
TCTTCTG443000.012.11379911
GTCGGAC1651.0586906E-56.7940291
Produced by FastQC (version 0.11.3)