FastQCFastQC Report
Sun 14 Aug 2016
V429_input.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameV429_input.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32888430
Sequences flagged as poor quality0
Sequence length79
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT50650.029.46551566
TATGCCG58300.026.03700363
TGCCGTC61100.024.12779465
CCGTCTT63250.023.76780167
CGTCTTC62600.023.72286468
CGTATGC66450.022.83786661
ATGCCGT65900.022.53639664
CTCGTAT71250.021.39435659
TCGTATG75300.020.44083460
TCTCGTA78900.019.08715458
ATCTCGT94650.015.56389657
GTATGCC109000.014.05795862
CCGAGAT138450.013.05599217
CCGATCT135450.011.442451554
ACCGATC155650.010.61715773
GATCTCG150200.010.46349656
CCGAGAC357250.010.436089573
CGAGATC175700.010.18437118
GACCGAT179600.09.8310173
GAGACCG268150.09.5775473
Produced by FastQC (version 0.11.3)