FastQCFastQC Report
Sun 14 Aug 2016
V429_H3K4me1_ChIPSeq.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameV429_H3K4me1_ChIPSeq.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40312350
Sequences flagged as poor quality0
Sequence length79
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCG1104930.2740921826685867Illumina Paired End PCR Primer 2 (100% over 31bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAG446330.11071793135354303Illumina Paired End PCR Primer 2 (96% over 33bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT192350.047.16118666
CGTCTTC199500.045.06522868
CCGTCTT203950.044.55076667
TATGCCG232150.038.7898163
ATGCCGT239750.037.53030864
TGCCGTC240550.037.42014765
CGTATGC266100.033.79941661
TCGTATG275800.032.59310560
CTCGTAT280150.032.0242459
TCTCGTA294250.030.50144258
GTATGCC312650.029.02252862
ATCTCGT326650.026.96957257
CCGAGAT381050.025.56707617
CGAGATC407100.023.7881818
GTCTTCT406900.022.40570869
CCGATCT416300.021.5152954
ACCGATC449300.019.98393453
GATCTCG451650.019.95159156
GACCGAT487150.018.46196652
CTTCTGC513400.018.01196571
Produced by FastQC (version 0.11.3)