##FastQC	0.11.3
>>Basic Statistics	pass
#Measure	Value
Filename	V429_H3K27ac_ChIPSeq.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	34387319
Sequences flagged as poor quality	0
Sequence length	79
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.391321521750505	40.0	38.0	40.0	35.0	40.0
2	38.22278192144029	40.0	38.0	40.0	35.0	40.0
3	38.18949907086388	40.0	38.0	40.0	35.0	40.0
4	38.19921838047334	40.0	38.0	40.0	35.0	40.0
5	38.22495083143876	40.0	38.0	40.0	35.0	40.0
6	38.25610365262846	40.0	38.0	40.0	35.0	40.0
7	38.17674247881901	40.0	38.0	40.0	35.0	40.0
8	38.199755758801665	40.0	38.0	40.0	35.0	40.0
9	37.812272076226705	40.0	38.0	40.0	34.0	40.0
10-11	38.2184369476434	40.0	38.0	40.0	35.0	40.0
12-13	38.19796063484914	40.0	38.0	40.0	35.0	40.0
14-15	38.145448224678404	40.0	38.0	40.0	35.0	40.0
16-17	38.19154924523194	40.0	38.0	40.0	35.0	40.0
18-19	38.08954396822852	40.0	38.0	40.0	34.5	40.0
20-21	38.0435349583374	40.0	38.0	40.0	34.5	40.0
22-23	37.979217004384665	40.0	38.0	40.0	34.0	40.0
24-25	37.89683977689566	40.0	38.0	40.0	33.0	40.0
26-27	37.733225713234575	39.0	38.0	40.0	33.0	40.0
28-29	37.59905431999511	39.0	38.0	40.0	33.0	40.0
30-31	37.468900090757295	39.0	38.0	40.0	33.0	40.0
32-33	37.274889720248325	39.0	37.5	40.0	33.0	40.0
34-35	37.111446199106126	39.0	37.0	40.0	32.5	40.0
36-37	36.93146805076604	39.0	36.5	40.0	32.0	40.0
38-39	36.79863633742427	39.0	36.0	40.0	31.0	40.0
40-41	36.59771570153521	39.0	36.0	40.0	31.0	40.0
42-43	36.4835798772216	39.0	36.0	40.0	30.5	40.0
44-45	36.34556325836277	39.0	36.0	40.0	30.0	40.0
46-47	36.5616961153616	39.0	36.0	40.0	31.0	40.0
48-49	36.436806748441185	39.0	36.0	40.0	31.0	40.0
50-51	36.30104593207746	39.0	36.0	40.0	30.5	40.0
52-53	36.1989263687582	39.0	36.0	40.0	30.5	40.0
54-55	36.02333829805109	39.0	36.0	40.0	30.0	40.0
56-57	35.754720075734895	39.0	35.5	40.0	29.5	40.0
58-59	35.520724689237916	39.0	35.0	40.0	29.0	40.0
60-61	35.296137480214725	38.0	35.0	40.0	29.0	40.0
62-63	34.926617309130734	38.0	34.5	40.0	28.0	40.0
64-65	34.643188394535784	38.0	34.0	40.0	27.0	40.0
66-67	34.45449136351688	38.0	34.0	40.0	27.0	40.0
68-69	34.206013559824186	38.0	33.5	40.0	26.0	40.0
70-71	33.76308962905773	38.0	33.0	39.0	24.0	40.0
72-73	33.28768136882087	37.0	33.0	39.0	21.5	40.0
74-75	32.8488876233707	36.5	33.0	39.0	18.0	40.0
76-77	32.355036968715126	36.0	32.5	39.0	2.0	40.0
78-79	31.837053173584135	36.0	31.5	39.0	2.0	40.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1	1	-0.3152503982607442
1	2	-0.4751095881862639
1	3	-0.4310836919821881
1	4	-0.3809244011417334
1	5	-0.3544143270595015
1	6	-0.31483268217633764
1	7	-0.3001369231852209
1	8	-0.35063092341039237
1	9	0.11646385110923063
1	10-11	-0.2345334292236032
1	12-13	-0.2691543280085966
1	14-15	-0.2570179873400136
1	16-17	-0.31276279879092783
1	18-19	-0.34998758004748964
1	20-21	-0.28328914779935843
1	22-23	-0.2904441731370824
1	24-25	-0.3109722116195428
1	26-27	-0.24542396027509739
1	28-29	-0.27199760632547054
1	30-31	-0.22476161945785122
1	32-33	-0.3090135894820776
1	34-35	-0.42895658751464794
1	36-37	-0.2945170769119443
1	38-39	-0.34850794180356104
1	40-41	-0.16684648124434887
1	42-43	-0.14280210177213348
1	44-45	-0.17811222673987714
1	46-47	0.009399909770692716
1	48-49	0.01708202445496454
1	50-51	0.10364357089580523
1	52-53	-0.02767778070109017
1	54-55	0.1171901100936239
1	56-57	-0.005332710833464205
1	58-59	0.0038286737717641017
1	60-61	0.17976709218564224
1	62-63	0.2782882463750269
1	64-65	0.3585920383859005
1	66-67	0.6086256300473138
1	68-69	0.6371266462852958
1	70-71	0.6717984158916437
1	72-73	1.025896016152906
1	74-75	1.0348628482696753
1	76-77	1.1460949363673834
1	78-79	0.9261384694579213
2	1	-0.3556951885538453
2	2	-0.3952113509713868
2	3	-0.4874858991709843
2	4	-0.35196851526644224
2	5	-0.4078619158283132
2	6	-0.2595590637160754
2	7	-0.489331416347234
2	8	-0.30351142352224514
2	9	0.11999149722836933
2	10-11	-0.27531921386862734
2	12-13	-0.2243396190210163
2	14-15	-0.2384307380248032
2	16-17	-0.3768080242866034
2	18-19	-0.3271887585947013
2	20-21	-0.2723182542098428
2	22-23	-0.2912773511272988
2	24-25	-0.31949323048172573
2	26-27	-0.23054144789804099
2	28-29	-0.2644043585005562
2	30-31	-0.2297450952657556
2	32-33	-0.2335001554631404
2	34-35	-0.4209140616773226
2	36-37	-0.3350917416253978
2	38-39	-0.1875208884331343
2	40-41	-0.17587107083724618
2	42-43	-0.12843247113066525
2	44-45	-0.23981007180307046
2	46-47	0.009166202887641361
2	48-49	0.03290493400600525
2	50-51	-0.019328317070268497
2	52-53	-0.026415168130519362
2	54-55	-0.016220602161745035
2	56-57	0.10617161015894538
2	58-59	0.11904986497475534
2	60-61	0.14270225943845816
2	62-63	-0.06450415982403968
2	64-65	0.42617411527379545
2	66-67	0.40940270444222193
2	68-69	0.4096573682894942
2	70-71	0.34961759030454687
2	72-73	0.7468963099616985
2	74-75	0.754621355364435
2	76-77	0.8113610727182419
2	78-79	0.6027361686722621
3	1	-0.2669547721580443
3	2	-0.44287074142393834
3	3	-0.36575564118398063
3	4	-0.42044048300954273
3	5	-0.40301361901364885
3	6	-0.3307865730836994
3	7	-0.2382966787989531
3	8	-0.2848777205152686
3	9	0.2003358251616305
3	10-11	-0.2323298919973027
3	12-13	-0.2849154565376537
3	14-15	-0.23338000846919016
3	16-17	-0.3157377388424294
3	18-19	-0.43815786651494193
3	20-21	-0.46033151446209075
3	22-23	-0.2845940481039477
3	24-25	-0.32716683239901556
3	26-27	-0.20663031185866032
3	28-29	-0.24666726467221167
3	30-31	-0.21083760878592273
3	32-33	-0.41187990318110934
3	34-35	-0.4548586659316598
3	36-37	-0.409019437419218
3	38-39	-0.38295800244898714
3	40-41	-0.4129723712965614
3	42-43	-0.2109571726679036
3	44-45	-0.15126050208587571
3	46-47	-0.06485028729031939
3	48-49	-0.07800300564926488
3	50-51	-0.08699979965866333
3	52-53	-0.02235761867957109
3	54-55	0.04429561427280504
3	56-57	-0.10245899516527146
3	58-59	-0.2823163862261069
3	60-61	-0.06230857350200125
3	62-63	0.2900748256554664
3	64-65	0.15709741818798761
3	66-67	0.38047079854115395
3	68-69	0.4977466728031956
3	70-71	0.5183592807776307
3	72-73	0.6716741463535882
3	74-75	0.6202532361750883
3	76-77	0.8100106130231595
3	78-79	0.6855700808312086
4	1	-0.22146703117824273
4	2	-0.3246074715824392
4	3	-0.3641410862884342
4	4	-0.4092382865386668
4	5	-0.3511627476066934
4	6	-0.19554537296511398
4	7	-0.31400743890142735
4	8	-0.17033990395584908
4	9	0.14708859351687664
4	10-11	-0.21060299538817873
4	12-13	-0.14273379753534954
4	14-15	-0.2253636322199668
4	16-17	-0.21595713251630855
4	18-19	-0.30212039200191043
4	20-21	-0.47673614893871985
4	22-23	-0.2543701143061341
4	24-25	-0.2895722661501168
4	26-27	-0.2300860792254653
4	28-29	-0.2192785239444035
4	30-31	-0.2936139917150058
4	32-33	-0.43447339299383003
4	34-35	-0.3609985832845837
4	36-37	-0.4551862429867981
4	38-39	-0.3244645429371147
4	40-41	-0.32240307752827846
4	42-43	-0.2805021043386233
4	44-45	-0.11822397763226178
4	46-47	-0.14109414802199893
4	48-49	-0.040391303138633816
4	50-51	-0.0862805213702913
4	52-53	-0.02202739568976142
4	54-55	-0.22684288428927601
4	56-57	-0.2787528685812859
4	58-59	-0.09043026650747521
4	60-61	-0.16118758135028344
4	62-63	0.05284005621849985
4	64-65	0.08872807315140108
4	66-67	0.3249090934938579
4	68-69	0.4195135840068289
4	70-71	0.47637477755745294
4	72-73	0.718145464622701
4	74-75	0.8129444957305481
4	76-77	0.8340878541341397
4	78-79	0.5862351065875337
5	1	-0.3269136550931222
5	2	-0.38876751261899045
5	3	-0.40609586783547513
5	4	-0.3776789772914739
5	5	-0.3621280149763635
5	6	-0.31759468508060706
5	7	-0.2394123852139245
5	8	-0.23445728605119598
5	9	0.1468022990783595
5	10-11	-0.23081900064914151
5	12-13	-0.19284660458145453
5	14-15	-0.2044570817659661
5	16-17	-0.24471949404980364
5	18-19	-0.326187989216983
5	20-21	-0.28013157746929807
5	22-23	-0.19167932872655769
5	24-25	-0.2776686189506705
5	26-27	-0.08200844598959378
5	28-29	-0.09711500737347478
5	30-31	-0.14037482157459635
5	32-33	-0.2056302709899711
5	34-35	-0.16994160207951126
5	36-37	-0.41136185374685397
5	38-39	-0.26659739699068297
5	40-41	-0.1777151820422347
5	42-43	-0.12803952269706542
5	44-45	0.028702440708620713
5	46-47	0.13530414492853282
5	48-49	0.19453108603777025
5	50-51	0.1793394431778239
5	52-53	0.12037074552984706
5	54-55	0.19600922855253344
5	56-57	-0.08150718297678594
5	58-59	0.05509813508881223
5	60-61	0.18632938467613513
5	62-63	0.35916327277023186
5	64-65	0.4054956924008053
5	66-67	0.5463267973864561
5	68-69	0.5906413061635973
5	70-71	0.6785047057104521
5	72-73	0.7147201075166905
5	74-75	0.8942952123750842
5	76-77	0.914746853445287
5	78-79	0.7999209113808767
6	1	-0.37332836898120547
6	2	-0.31753227328555766
6	3	-0.507166763363152
6	4	-0.4585169945333263
6	5	-0.36327792118505897
6	6	-0.3153888365828976
6	7	-0.32520090516167954
6	8	-0.23123393561097316
6	9	0.16437855323299289
6	10-11	-0.17664393277459567
6	12-13	-0.1793532590016227
6	14-15	-0.22487146863389285
6	16-17	-0.23228215753856318
6	18-19	-0.21857965956179726
6	20-21	-0.18346424080703372
6	22-23	-0.18124632407086239
6	24-25	-0.1438300446705938
6	26-27	-0.08521220532124829
6	28-29	-0.09893620268423575
6	30-31	-0.12843625052328633
6	32-33	-0.0534956159219675
6	34-35	-0.09921607556775314
6	36-37	-0.215873153439901
6	38-39	-0.027328702102245472
6	40-41	-0.13304961166507212
6	42-43	-0.015217918861381463
6	44-45	0.07417866299849862
6	46-47	0.11123071294854014
6	48-49	0.14115984284594418
6	50-51	0.10137833906655658
6	52-53	0.11868188433865612
6	54-55	0.09656502130467715
6	56-57	0.1453137426342508
6	58-59	0.18068398729403157
6	60-61	0.1828954394990916
6	62-63	0.3769663726689032
6	64-65	0.36083184201869756
6	66-67	0.5708188357610595
6	68-69	0.8014607703096956
6	70-71	0.8752330061808706
6	72-73	0.9682130813531202
6	74-75	0.9991513883068777
6	76-77	1.205798124396054
6	78-79	1.0982877938549045
7	1	-0.22736810600946455
7	2	-0.2316924943375156
7	3	-0.38837406605135527
7	4	-0.23580513568600736
7	5	-0.30890957429564736
7	6	-0.11353785997572885
7	7	-0.239806952142672
7	8	-0.17376457178956883
7	9	0.2600955473209652
7	10-11	-0.1505192539508613
7	12-13	-0.15358120314215995
7	14-15	-0.21618106713916063
7	16-17	-0.18278433430342034
7	18-19	-0.209464753125566
7	20-21	-0.2713363455729052
7	22-23	-0.1465677620873791
7	24-25	-0.14201906300372968
7	26-27	-0.020793738513170013
7	28-29	-0.027228239232499618
7	30-31	-0.07641531810111246
7	32-33	-0.17743765574039827
7	34-35	-0.04234027381612293
7	36-37	-0.14002342643330223
7	38-39	-0.06715116138932586
7	40-41	0.025467093431217336
7	42-43	0.024362530650037684
7	44-45	0.06823936990209489
7	46-47	0.19328052181836597
7	48-49	0.15412899845242833
7	50-51	0.12193407298449443
7	52-53	-0.003723719564312944
7	54-55	0.09324588575645265
7	56-57	0.02224974307502947
7	58-59	0.11878976345633419
7	60-61	0.27835151126823376
7	62-63	0.293238678267258
7	64-65	0.4576581839479985
7	66-67	0.5983842331511795
7	68-69	0.7791140334301474
7	70-71	0.8754896706135114
7	72-73	0.9806500355604086
7	74-75	1.0787517438983087
7	76-77	1.0261910699031631
7	78-79	0.9269582848066271
8	1	-0.18202810149480797
8	2	-0.1705459499443549
8	3	-0.2397336010881972
8	4	-0.14794789716979295
8	5	-0.19468069129413834
8	6	-0.1506887007534914
8	7	-0.09911979795575832
8	8	-0.13966148843037018
8	9	0.32145102799672287
8	10-11	-0.14265901805457304
8	12-13	-0.0860031874037972
8	14-15	-0.07687344317955791
8	16-17	-0.13251266346196644
8	18-19	-0.09472569497982164
8	20-21	-0.08057050771950713
8	22-23	-0.08882988473644815
8	24-25	-0.02745860042686843
8	26-27	-0.03812506344683442
8	28-29	-0.043412025955774425
8	30-31	0.019876857001051462
8	32-33	0.02753030749566676
8	34-35	-0.05066003492829907
8	36-37	0.08569524115887361
8	38-39	0.039812442338742926
8	40-41	0.1212136006375033
8	42-43	0.10552069863753388
8	44-45	0.1854121389333585
8	46-47	0.2149854625687908
8	48-49	0.24926031890215938
8	50-51	0.30189121343553893
8	52-53	0.2631207745206652
8	54-55	0.04468813124911719
8	56-57	0.21942647945593308
8	58-59	0.260475043388368
8	60-61	0.42319202071345785
8	62-63	0.5192455980633
8	64-65	0.7132452614581553
8	66-67	0.6804266935422589
8	68-69	0.7437350265076574
8	70-71	0.9108376329459986
8	72-73	0.9969487408846831
8	74-75	1.1436870227313491
8	76-77	1.1770449431290615
8	78-79	1.214666234694942
9	1	-0.07254475133512273
9	2	-0.09446837837231215
9	3	-0.2153940965897263
9	4	-0.14696915465583515
9	5	-0.10515418912434171
9	6	-0.11456954538036968
9	7	-0.12006799415896552
9	8	-0.024451324034792776
9	9	0.3573789218957515
9	10-11	-0.037377867814520016
9	12-13	-0.013685378214312038
9	14-15	-0.04034805413804321
9	16-17	-0.06324734688070777
9	18-19	-0.1337772511646591
9	20-21	-0.07605282766341048
9	22-23	-0.09007060819854473
9	24-25	-0.12562702232461476
9	26-27	-0.05549669259466583
9	28-29	-0.030682145458555965
9	30-31	-0.052615068410730714
9	32-33	-0.045414335100865344
9	34-35	-0.07953098820804883
9	36-37	-0.007069049896216484
9	38-39	0.01044101278115761
9	40-41	-0.0620704166488224
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117	68-69	0.18924293946076176
117	70-71	0.31892522755969566
117	72-73	0.4775530428796415
117	74-75	0.49131060398561743
117	76-77	0.49300728430979746
117	78-79	0.39170296413733396
118	1	-0.15191303158436398
118	2	-0.22841507105319891
118	3	-0.1231723936266178
118	4	-0.2018824676156612
118	5	-0.2276415848509643
118	6	-0.1174939216984825
118	7	-0.13587363100896255
118	8	-0.14471952208673144
118	9	0.253188679410961
118	10-11	-0.12215500787989697
118	12-13	-0.15760840932588138
118	14-15	-0.1616564494404713
118	16-17	-0.17801511637827616
118	18-19	-0.20976965886491428
118	20-21	-0.21336831103993603
118	22-23	-0.1649658124647999
118	24-25	-0.16830498931373228
118	26-27	-0.17739263404758532
118	28-29	-0.21080214471322023
118	30-31	-0.20560423691905783
118	32-33	-0.15850661988675796
118	34-35	-0.28957077532160724
118	36-37	-0.23342219580148793
118	38-39	-0.18258547268798964
118	40-41	-0.21639926790913222
118	42-43	-0.22339656306009914
118	44-45	-0.2791509774577534
118	46-47	-0.0956649649852892
118	48-49	-0.16690813948466143
118	50-51	-0.028901422556721457
118	52-53	0.020132570066827782
118	54-55	0.018698955661271555
118	56-57	-0.16694004482029356
118	58-59	-0.21789684243249496
118	60-61	-0.08859353766211342
118	62-63	0.15478994067052554
118	64-65	0.12138247270112856
118	66-67	0.21267401441708245
118	68-69	0.2045244375429931
118	70-71	0.23798006909397884
118	72-73	0.43660176325007427
118	74-75	0.4380512956790312
118	76-77	0.37751943859154835
118	78-79	0.16696114669276696
119	1	-0.24738522966726606
119	2	-0.3021997337699531
119	3	-0.2850298241136002
119	4	-0.32051216263779736
119	5	-0.25534088660691623
119	6	-0.25683926220987985
119	7	-0.25090654431490833
119	8	-0.22098079474699261
119	9	0.213555755683835
119	10-11	-0.18061004005561188
119	12-13	-0.15329196461554062
119	14-15	-0.14767938380223455
119	16-17	-0.1993476519470505
119	18-19	-0.20509180632060975
119	20-21	-0.25942482639342757
119	22-23	-0.2559981016570205
119	24-25	-0.2829422668526007
119	26-27	-0.299514220941127
119	28-29	-0.3316749505751986
119	30-31	-0.2689833394383072
119	32-33	-0.2740130704537478
119	34-35	-0.27528650340477867
119	36-37	-0.3393309656032315
119	38-39	-0.28465600798513435
119	40-41	-0.2863464450436055
119	42-43	-0.33114396309721883
119	44-45	-0.28715603273645485
119	46-47	-0.19625697007026588
119	48-49	-0.3202205464296668
119	50-51	-0.21113075322609376
119	52-53	-0.21876090471709375
119	54-55	-0.16766181230149613
119	56-57	-0.18381873705818208
119	58-59	-0.1733495524828257
119	60-61	-0.3044688988599873
119	62-63	-0.19796453685945892
119	64-65	0.12234688161726126
119	66-67	-0.09350557607845644
119	68-69	0.06135363433762819
119	70-71	0.06138671047209243
119	72-73	0.24481798393114929
119	74-75	0.2837943300951622
119	76-77	0.3481846262517365
119	78-79	0.11205363732904416
120	1	-0.28460224765823483
120	2	-0.32033092923673934
120	3	-0.32567993974898
120	4	-0.3298285885980974
120	5	-0.41700369787659497
120	6	-0.22032823739106533
120	7	-0.24584900772731544
120	8	-0.21168254491605154
120	9	0.1774860576620938
120	10-11	-0.17854013088891918
120	12-13	-0.1410921259556588
120	14-15	-0.15110480891510747
120	16-17	-0.24941530600025885
120	18-19	-0.32860570478959517
120	20-21	-0.28143968647281525
120	22-23	-0.3069788989802049
120	24-25	-0.2206254898860749
120	26-27	-0.18245893677863023
120	28-29	-0.2764451938354213
120	30-31	-0.227097942687152
120	32-33	-0.2508387820254825
120	34-35	-0.2448176891473679
120	36-37	-0.24585747892503207
120	38-39	-0.16733303831169621
120	40-41	-0.14557925756627554
120	42-43	-0.06691335880034899
120	44-45	-0.13880063197257897
120	46-47	-0.10528324711123105
120	48-49	-0.06692501225276004
120	50-51	-0.11056478935541492
120	52-53	0.013323590572326793
120	54-55	0.024528342366643585
120	56-57	0.002196153418914548
120	58-59	0.08743973393701765
120	60-61	0.024716451325957678
120	62-63	0.08598756515448258
120	64-65	0.23885995586393705
120	66-67	0.4110562350271536
120	68-69	0.4252673095497457
120	70-71	0.5130137461315201
120	72-73	0.6379859754478119
120	74-75	0.6978207742468499
120	76-77	0.7783137667959679
120	78-79	0.5300586086271757
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	50462.0
3	2003.0
4	3321.0
5	6956.0
6	9377.0
7	14655.0
8	20861.0
9	30047.0
10	39784.0
11	47898.0
12	59053.0
13	70378.0
14	69883.0
15	79174.0
16	82600.0
17	90428.0
18	98426.0
19	104633.0
20	116369.0
21	125856.0
22	144201.0
23	163579.0
24	190284.0
25	224874.0
26	262144.0
27	312556.0
28	366286.0
29	429268.0
30	491640.0
31	581869.0
32	713355.0
33	906900.0
34	1251636.0
35	1719970.0
36	2491404.0
37	3945308.0
38	6366301.0
39	1.2701671E7
40	1909.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	18.71322825283734	24.093609255069655	23.350387400829124	33.84277509126388
2	22.710121716671196	24.217427360359206	27.932930741125823	25.13952018184378
3	23.752238434173947	25.49055947048387	26.185626742230184	24.571575353112
4	23.31275539219296	24.714240498267557	26.661328069065483	25.311676040474
5	24.10580481717694	26.29189265961676	27.01072159769129	22.591580925515014
6	23.51785261305192	25.801752675164934	26.081890827255243	24.598503884527897
7	23.67912427252616	26.116708894927225	26.98710533380052	23.217061498746094
8	22.660941188109696	25.219283469320814	27.413041473591658	24.706733868977825
9	22.578993188023745	24.52096398758733	28.074762606334914	24.82528021805401
10-11	23.51117487622471	25.67002612242258	26.25972175321854	24.559077248134166
12-13	23.58272425341918	25.65541858112606	25.997461825934465	24.764395339520295
14-15	23.452947405826567	25.78952853205966	26.00859100985377	24.748933052260007
16-17	23.866646593357405	25.54192700511887	25.804063117742814	24.787363283780913
18-19	23.93153649130145	25.6893011249222	25.684358894995324	24.694803488781027
20-21	23.894322235129284	25.862210632733625	25.722613998913346	24.520853133223742
22-23	23.698376521046402	25.80708946614679	26.161842530224632	24.332691482582177
24-25	23.752513342326857	25.717487162268377	25.98864529848844	24.541354196916327
26-27	23.84881796124147	25.70548227068443	26.079798957587606	24.36590081048649
28-29	23.902176478342742	25.79319046971901	25.8284504606803	24.476182591257945
30-31	23.954696249495836	25.8372631126762	25.878483679047427	24.32955695878054
32-33	24.042793382471185	25.62905220309289	25.767780413792206	24.56037400064372
34-35	24.038923015759714	25.741634068805308	25.922424652604587	24.297018262830385
36-37	24.037973035495476	25.774032698008963	25.895809165218253	24.292185101277312
38-39	24.059418089022543	26.07000367079133	25.736899177028512	24.13367906315761
40-41	24.003915736889105	25.85783078719473	25.76179346777491	24.37646000814125
42-43	24.247583413727796	25.67975067989431	25.836774954122106	24.235890952255794
44-45	24.015301414684888	25.971489002971794	25.821361997826425	24.191847584516893
46-47	24.11984320690128	25.802183983700445	25.96182859625428	24.116144213143993
48-49	23.96405040451662	25.851676270298583	25.660178394738374	24.524094930446424
50-51	24.307896251360216	25.807794120062383	25.649487335217174	24.23482229336022
52-53	24.29203814356871	26.058493778405158	25.57564672458422	24.07382135344191
54-55	24.21072330044325	25.778019914042822	25.67809457471821	24.333162210795724
56-57	24.260977413466296	25.88855680398255	25.683260649948014	24.167205132603144
58-59	24.2867580649422	25.811927366730952	25.666857265613626	24.234457302713217
60-61	24.33874522431329	25.782941483365423	25.616378197513466	24.261935094807818
62-63	24.389440649632157	25.754307524868075	25.634007644468266	24.222244181031506
64-65	24.30391991814492	25.973192903821502	25.601512396304184	24.12137478172939
66-67	24.49511811780915	25.732733518545338	25.470398601113313	24.301749762532197
68-69	24.435784723666664	25.798242056104403	25.463843622541667	24.302129597687266
70-71	24.436579250680474	25.680792716250632	25.596188803923564	24.28643922914533
72-73	24.473156764599562	25.713176966234474	25.66494840182735	24.14871786733862
74-75	24.524020302975025	25.74417332180838	25.54429383344386	24.187512541772733
76-77	24.862543019834416	25.67261960214637	25.34010527292138	24.124732105097834
78-79	24.715080458633512	25.626592512838137	25.471950974267155	24.186376054261196
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	204.0
1	132.5
2	64.0
3	101.5
4	160.0
5	184.0
6	254.0
7	419.0
8	673.5
9	1071.0
10	1309.0
11	1675.5
12	2804.0
13	4635.5
14	5705.0
15	7805.0
16	13410.5
17	22535.5
18	36322.0
19	44489.0
20	55995.0
21	83267.0
22	119904.5
23	166467.0
24	192158.0
25	223965.0
26	291885.5
27	368483.0
28	453051.5
29	497136.0
30	542428.0
31	634398.0
32	730330.5
33	779585.0
34	826859.5
35	921822.0
36	1010476.0
37	1089634.5
38	1127827.0
39	1158214.5
40	1211823.0
41	1252286.0
42	1281250.0
43	1292972.0
44	1303380.0
45	1318972.5
46	1319977.5
47	1304048.0
48	1292298.0
49	1272099.0
50	1227659.5
51	1178748.0
52	1154077.0
53	1130250.5
54	1092721.5
55	1061094.0
56	1059534.0
57	1075899.0
58	1029460.5
59	937084.5
60	830476.5
61	727404.0
62	685002.0
63	647822.0
64	577031.5
65	513211.0
66	453920.5
67	424840.0
68	398623.5
69	352062.0
70	311922.0
71	292127.0
72	276269.0
73	246167.0
74	219376.5
75	195593.0
76	184356.0
77	173189.5
78	151186.5
79	129882.0
80	109071.0
81	98728.0
82	89019.5
83	70442.5
84	53422.5
85	38926.5
86	32582.0
87	27400.0
88	18187.5
89	11600.0
90	9043.0
91	7107.0
92	4031.5
93	2207.5
94	1133.0
95	743.0
96	532.0
97	224.5
98	86.5
99	39.0
100	33.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.40930204532665077
2	0.0
3	0.0
4	3.198853623918747E-5
5	0.0
6	0.0
7	0.0
8	6.04874139795545E-4
9	0.06898764047293132
10-11	8.72414624705113E-6
12-13	1.4540243745085216E-6
14-15	9.465698678050475E-4
16-17	9.014951121952833E-5
18-19	2.1810365617627824E-5
20-21	8.287938934698573E-5
22-23	8.753226734541301E-4
24-25	0.00966635404173265
26-27	0.06407303808709251
28-29	0.06981934241515018
30-31	0.0539530284405132
32-33	0.03545202229926677
34-35	0.022575182438619307
36-37	0.024267666810547227
38-39	0.0425200347837527
40-41	0.04136117735726941
42-43	0.030442908329084913
44-45	0.031287696490674366
46-47	0.07742098184508074
48-49	0.07362888627636252
50-51	0.05044155957607512
52-53	0.04917946641900173
54-55	0.055716760006792036
56-57	0.04581485401638901
58-59	0.04353639782153416
60-61	0.03954219286475925
62-63	0.04327903550724615
64-65	0.05317367137577663
66-67	0.0980434095487351
68-69	0.13362484001733313
70-71	0.13583059499346256
72-73	0.12668623570217846
74-75	0.10067519366659552
76-77	0.07937809865316921
78-79	0.06972773887955616
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
79	3.4387319E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	70.00675271917233
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.15332428131039	50.51219930827949
2	19.287185068009936	27.00466391410169
3	5.606508860734995	11.7748043829397
4	1.7652007930192573	4.943039016663441
5	0.638344623037582	2.2344217087302636
6	0.258627025342388	1.0863382925783804
7	0.1285830514929966	0.630117732282277
8	0.06359236392008023	0.35615159166245386
9	0.03571965705165789	0.22505554785861487
>10	0.06192712562741641	0.6163540299667899
>50	7.039007464078979E-4	0.030400454510853683
>100	2.2475495140045668E-4	0.025970246195160095
>500	2.91608899699369E-5	0.013962529184564169
>1k	1.6852992327080208E-5	0.028285370555196712
>5k	0.0	0.0
>10k+	1.2480873213737211E-5	0.5182358744910867
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCG	88638	0.25776362501537264	Illumina Paired End PCR Primer 2 (100% over 31bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAG	45577	0.13254013783394977	Illumina Paired End PCR Primer 2 (97% over 36bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAG	43721	0.12714279935577413	Illumina Paired End PCR Primer 2 (96% over 33bp)
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.1050585246264764E-4	2.9080487490170432E-6	8.72414624705113E-6	0.0
2	1.1341390121166468E-4	5.8160974980340864E-6	2.617243874115339E-5	0.0
3	1.3086219370576693E-4	5.8160974980340864E-6	3.489658498820452E-5	2.9080487490170432E-6
4	1.3958633995281807E-4	5.8160974980340864E-6	4.9436828733289735E-5	2.9080487490170432E-6
5	1.6575877869397145E-4	5.8160974980340864E-6	6.979316997640903E-5	8.72414624705113E-6
6	1.7739097369003963E-4	5.8160974980340864E-6	7.851731622346017E-5	8.72414624705113E-6
7	1.948392661841419E-4	5.8160974980340864E-6	8.433341372149426E-5	1.1632194996068173E-5
8	2.2391975367431233E-4	1.1632194996068173E-5	8.72414624705113E-5	1.744829249410226E-5
9	6.310465785366984E-4	1.4540243745085216E-5	9.014951121952833E-5	1.744829249410226E-5
10	7.357363335013119E-4	1.4540243745085216E-5	9.596560871756242E-5	2.3264389992136346E-5
11	9.131273071913516E-4	1.4540243745085216E-5	1.075978037136306E-4	2.9080487490170433E-5
12	0.0013493346195439081	2.0356341243119302E-5	1.2213804745871582E-4	2.9080487490170433E-5
13	0.001555806080724118	2.3264389992136346E-5	1.279541449567499E-4	2.9080487490170433E-5
14	0.0018349787606297542	2.617243874115339E-5	1.3667829120380103E-4	3.489658498820452E-5
15	0.015595865440978402	2.617243874115339E-5	1.483104861998692E-4	3.489658498820452E-5
16	0.016442107626942364	2.617243874115339E-5	1.5121853494888624E-4	3.780463373722156E-5
17	0.01864640857869728	2.617243874115339E-5	1.6285072994495442E-4	4.0712682486238604E-5
18	0.020245835390656653	2.617243874115339E-5	1.6866682744298852E-4	4.0712682486238604E-5
19	0.022636251462348662	2.617243874115339E-5	1.890231686861078E-4	4.652877998427269E-5
20	0.30276858745516044	2.617243874115339E-5	1.9774731493315894E-4	4.9436828733289735E-5
21	0.3149504036647928	2.9080487490170433E-5	2.1228755867824415E-4	4.9436828733289735E-5
22	0.3177799350975864	2.9080487490170433E-5	2.210117049252953E-4	5.525292623132382E-5
23	0.32070252409034855	3.198853623918747E-5	2.4427609491743164E-4	5.8160974980340866E-5
24	0.3240555042979652	3.198853623918747E-5	2.530002411644828E-4	6.397707247837495E-5
25	0.32804825523036557	3.780463373722156E-5	2.646324361605509E-4	6.6885121227392E-5
26	0.33336416834356875	4.362073123525565E-5	2.8789682615268727E-4	7.270121872542608E-5
27	0.3402882324149783	4.362073123525565E-5	2.8789682615268727E-4	8.142536497247721E-5
28	0.48643513034557884	4.362073123525565E-5	2.995290211487554E-4	8.433341372149426E-5
29	0.49776488827174925	4.362073123525565E-5	3.024370698977725E-4	9.596560871756242E-5
30	0.51172643031578	4.362073123525565E-5	3.0825316739580656E-4	9.887365746657947E-5
31	0.664942794755241	4.362073123525565E-5	3.111612161448236E-4	1.0468975496461356E-4
32	0.6776160712034573	4.9436828733289735E-5	3.111612161448236E-4	1.075978037136306E-4
33	0.6910919691064022	4.9436828733289735E-5	3.2570145988990883E-4	1.1632194996068173E-4
34	0.7083628706268145	4.9436828733289735E-5	3.3442560613695997E-4	1.1922999870969877E-4
35	0.7302081328294305	5.234487748230678E-5	3.431497523840111E-4	1.1922999870969877E-4
36	0.7567208132742189	5.234487748230678E-5	3.5187389863106225E-4	1.2213804745871582E-4
37	0.7874152678200937	5.234487748230678E-5	3.6059804487811333E-4	1.2504609620773286E-4
38	0.8209043572137741	5.234487748230678E-5	3.751382886231986E-4	1.279541449567499E-4
39	0.8555217695220729	5.525292623132382E-5	3.8677048361926674E-4	1.3958633995281807E-4
40	0.8920235974197349	5.525292623132382E-5	3.984026786153349E-4	1.424943887018351E-4
41	0.9307180940741556	5.525292623132382E-5	4.04218776113369E-4	1.424943887018351E-4
42	0.9702588329145404	6.106902372935791E-5	4.04218776113369E-4	1.5121853494888624E-4
43	1.0123877351415502	6.106902372935791E-5	4.04218776113369E-4	1.5121853494888624E-4
44	1.0576747783099927	6.106902372935791E-5	4.0712682486238603E-4	1.5412658369790328E-4
45	1.1076292397206076	6.106902372935791E-5	4.1875901985845423E-4	1.5412658369790328E-4
46	1.1616666015748423	6.106902372935791E-5	4.2166706860747124E-4	1.5703463244692034E-4
47	1.2182688624257099	6.106902372935791E-5	4.2166706860747124E-4	1.6575877869397145E-4
48	1.2765112627710233	6.6885121227392E-5	4.3620731235255645E-4	1.6866682744298852E-4
49	1.3375977347928751	6.6885121227392E-5	4.391153611015735E-4	1.6866682744298852E-4
50	1.3997340124131223	6.6885121227392E-5	4.4783950734862465E-4	1.744829249410226E-4
51	1.4631701878241803	6.6885121227392E-5	4.594717023446928E-4	1.7739097369003963E-4
52	1.5274322490799588	6.6885121227392E-5	4.71103897340761E-4	1.7739097369003963E-4
53	1.5919211381381608	6.6885121227392E-5	4.71103897340761E-4	1.7739097369003963E-4
54	1.6570497979211465	6.6885121227392E-5	4.8273609233682915E-4	1.7739097369003963E-4
55	1.7248218740169887	6.6885121227392E-5	4.943682873328973E-4	1.8320707118807373E-4
56	1.7927422605990306	6.6885121227392E-5	5.030924335799484E-4	1.8320707118807373E-4
57	1.8628582239865807	6.6885121227392E-5	5.030924335799484E-4	1.8320707118807373E-4
58	1.934745189062282	6.6885121227392E-5	5.205407260740507E-4	1.8611511993709077E-4
59	2.0059051419507288	6.6885121227392E-5	5.234487748230677E-4	1.890231686861078E-4
60	2.07791133702514	6.6885121227392E-5	5.583453598112723E-4	1.890231686861078E-4
61	2.152188718172533	6.6885121227392E-5	5.728856035563575E-4	1.9774731493315894E-4
62	2.2260851449338053	6.6885121227392E-5	5.757936523053745E-4	1.9774731493315894E-4
63	2.30024038803374	6.6885121227392E-5	5.787017010543916E-4	2.0647146118021008E-4
64	2.374331654061196	6.6885121227392E-5	5.932419447994768E-4	2.0937950992922711E-4
65	2.4484432764298956	6.6885121227392E-5	5.990580422975108E-4	2.1228755867824415E-4
66	2.5226334161148185	6.6885121227392E-5	6.07782188544562E-4	2.151956074272612E-4
67	2.597812292374407	6.6885121227392E-5	6.281385297876814E-4	2.2391975367431233E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGTCTT	18350	0.0	37.026478	67
GCCGTCT	18655	0.0	36.40148	66
CGTCTTC	18720	0.0	36.274925	68
TATGCCG	21000	0.0	32.57761	63
ATGCCGT	21490	0.0	31.666815	64
TGCCGTC	22605	0.0	30.121128	65
CGTATGC	26520	0.0	25.77378	61
CTCGTAT	26720	0.0	25.60881	59
TCGTATG	26795	0.0	25.558004	60
TCTCGTA	27610	0.0	24.62313	58
GTATGCC	28895	0.0	23.761158	62
ATCTCGT	30150	0.0	22.620903	57
GTCTTCT	30995	0.0	22.380224	69
CCGAGAT	33315	0.0	21.631857	17
CGAGATC	33870	0.0	21.212877	18
ACCGATC	33915	0.0	20.359829	53
CCGATCT	33835	0.0	20.35371	54
GACCGAT	34965	0.0	19.831896	52
AGACCGA	39200	0.0	17.84767	51
GATCTCG	38760	0.0	17.765911	56
>>END_MODULE
