FastQCFastQC Report
Sun 14 Aug 2016
V429_H3K27ac_ChIPSeq.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameV429_H3K27ac_ChIPSeq.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34387319
Sequences flagged as poor quality0
Sequence length79
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCG886380.25776362501537264Illumina Paired End PCR Primer 2 (100% over 31bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAG455770.13254013783394977Illumina Paired End PCR Primer 2 (97% over 36bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAG437210.12714279935577413Illumina Paired End PCR Primer 2 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCTT183500.037.02647867
GCCGTCT186550.036.4014866
CGTCTTC187200.036.27492568
TATGCCG210000.032.5776163
ATGCCGT214900.031.66681564
TGCCGTC226050.030.12112865
CGTATGC265200.025.7737861
CTCGTAT267200.025.6088159
TCGTATG267950.025.55800460
TCTCGTA276100.024.6231358
GTATGCC288950.023.76115862
ATCTCGT301500.022.62090357
GTCTTCT309950.022.38022469
CCGAGAT333150.021.63185717
CGAGATC338700.021.21287718
ACCGATC339150.020.35982953
CCGATCT338350.020.3537154
GACCGAT349650.019.83189652
AGACCGA392000.017.8476751
GATCTCG387600.017.76591156
Produced by FastQC (version 0.11.3)