FastQCFastQC Report
Sun 14 Aug 2016
V429_DNase.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameV429_DNase.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11165630
Sequences flagged as poor quality0
Sequence length71
%GC34

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCTTG21450.050.30145336
TCTTCCG22250.049.9231232
TTGCAAC38100.049.5076540
GAAAAAC317350.049.0957742
GGCGTTT33800.048.61091627
CTTCCGC24000.048.5878833
TGGCGTT33800.048.4155126
GCGTTTT37650.048.13458628
TGAAAAC258850.048.0062940
TCTGCCG93800.047.7355624
CGCGTAA1100.047.4345664
TTCCGCT21750.047.04162634
CTGCCGT88050.046.5510725
CTTTTGC413150.046.34691233
TTGCTTG426550.046.31609336
TCCGCTT21050.046.2978535
TCGCATG16650.046.16681722
CGCGAAA3250.046.15747564
GCGACGA2900.046.11665
CGGTCTT28250.046.0988929
Produced by FastQC (version 0.11.3)